Glasgow’s Modified Eagle’s Medium

  • Advanced Glasgow’s Modified Eagle’s Medium

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    Advanced Glasgow’s Modified Eagle’s Medium

    Background

    PurMaTM Advanced Glasgow’s Modified Eagle’s Medium is free of glutamine amino acids. Moreover, it contains components that allow for a 60–90% reduction in FBS supplementation. More importantly, using synthetic components such as recombinant insulin, albumin, and transferrin increase consistency. This results in less variation amongst lot-to-lot serum changes. In A-GMEM, L-Alanyl-L-Glutamine is used as the source of glutamine. Additionally, reducing FBS requirements lowers the cost of cell culture experiments.

    Contents

    Advanced Glasgow’s Contains:

    • includes all twenty essential and non-essential amino acids with higher concentrations than regular and MAX formulations.
    • A-GMEM incorporates elements that significantly reduce the need for a serum for cell culture.

    Crucial Ingredients:

    • PurMaTM Human Transferrin (Holo): prevents Improper iron storage and delivery in cell culture systems which is a major contributor to oxidative stress and protein damage.
    • PurMaTM Insulin Recombinant
    • Trace of other elements which mimic the elements in fetal bovine serum.
    Consequently, Advanced Glasgow’s Modified Eagle’s Medium  is a significant step toward a chemically defined form of this media. Unquestionably, Chemically Defined  Glasgow’s medium  (CD-Glasgow’s meium) is the outcome of decades of cutting-edge research and will soon be offered globally to laboratories that are looking for consistency and repeatability in their data.

    Formulation

    Complete formulation is available here: Advanced Glasgow’s Formulation 

    References

    [/fusion_text][fusion_table fusion_table_type="2" fusion_table_rows="13" fusion_table_columns="2" margin_top="30px" margin_right="" margin_bottom="" margin_left="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" class="" id="" animation_type="" animation_direction="left" animation_color="" hue="" saturation="" lightness="" alpha="" animation_speed="0.3" animation_delay="0" animation_offset=""]
    Parameter Specification
    Appearance Red, clear liquid
    pH 7.2 ± 0.1
    Osmolality 275-360 mOsm/L
    Endotoxin NMT< 2EU/mL
    Mycoplasma Negative
    Suitability  Suitable for mammalian cell culture
    Additive Sodium pyruvate
    Indicator Phenol red
    Mycoplasma Detection Negative
    Sterility Tested Sterile filtered using 0.22 µm filter
    Form Liquid
    Shipping Condition  Room temperature
    [/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed Read more

  • Glasgow’s Modified Eagle’s Medium

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    Glasgow’s Modified Eagle’s Medium

    Background

    Ian McPherson and Michael Stoker developed Glasgow’s Modified Eagle’s Medium . they used  polyoma virus to transform four fibroblast clones from a culture of baby hamster kidney cells.  as a result to Particular criteria mentioned below Glasgow Minimum Essential Medium (GMEM) is used without serum. Needs to be supplemented with 10% Tryptose phosphate broth. Moreover, it contains twice amount of amino acids and vitamins compared to the original Basal Medium Eagle.

    PurMa™ Glasgow’s Modified Eagle’s Medium also Contains

    • 75 g/L Sodium Bicarbonate
    • High Glucose (4.5g/L)
    PurMa Biologics manufactures 77 types of GMEM

    Why PurMa Biologics? Why we stand behind our cell culture media

    Our products are the outcome of 30 years of experience in cutting edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. We utilize %99 pure amino acids. This will significantly increase the consistency among your experiments, and the result of your lab. Considering the highest quality, our prices are extremely reasonable.

    Application of this media

    It is suitable for growth of primate embryonic stem cells, MDCK, NS0, BHK-21 as well as CHO cells containing the GS expression system. Alternative to 10% Tryptose phosphate broth, we strongly recommend PurMa TM 50X GS Supplement. This is certainly a enriched mixture of amino acids and nucleosides, and Fetal Bovine Serum (FBS) (Cat P3S528332). furthermore, this media has been formulated for use in a 5% carbon dioxide atmosphere. As a result, It utilizes a bicarbonate buffering system.

    Formulation

    The Complete formulation is available in " formulation tab" as well as by clicking here: Glasgow’s (GMEM) Formulation

    References

    [/fusion_text][fusion_table fusion_table_type="2" fusion_table_rows="13" fusion_table_columns="2" margin_top="30px" margin_right="" margin_bottom="" margin_left="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" class="" id="" animation_type="" animation_direction="left" animation_color="" hue="" saturation="" lightness="" alpha="" animation_speed="0.3" animation_delay="0" animation_offset=""]
    Parameter Specification
    Appearance Red, clear liquid
    pH 7.2 ± 0.1
    Osmolality 275-360 mOsm/L
    Endotoxin NMT< 2EU/mL
    Mycoplasma Negative
    Suitability  Suitable for mammalian cell culture
    Additive Sodium pyruvate
    Indicator Phenol red
    Mycoplasma Detection Negative
    Sterility Tested Sterile filtered using 0.22 µm filter
    Form Liquid
    Shipping Condition  Room temperature
    [/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed Read more





Ian McPherson and Michael Stoker developed Glasgow’s Modified Eagle’s Medium. they used polyoma virus to transform four fibroblast clones from a culture of baby hamster kidney cells. As a result of the Particular criteria mentioned below this media is used without serum. Needs to be supplemented with 10% Tryptose phosphate broth.

Moreover, it contains twice the amount of amino acids and vitamins compared to the original Basal Medium Eagle.

Glasgow’s Modified Eagle’s Medium   also Contains

  • 75 g/L Sodium Bicarbonate
  • High Glucose (4.5g/L)

PurMa Biologics manufactures 77 types of GMEM

Our products are the outcome of 30 years of experience in cutting-edge science. PurMa Biologics routinely manufactures over 1500 media. We have the most comprehensive collection of cell and tissue culture media. we use %99 pure amino acids. This will significantly increase the consistency among your experiments, and the result of your lab. Considering the highest quality, our prices are extremely reasonable.

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