Advanced Glasgow’s Modified Eagle’s Medium
Advanced Glasgow’s Modified Eagle’s Medium is free of glutamine contains L-Alanyl-L-Glutamine as well as ingredients to allow for serum reduction. Additionally, Advanced Glasgow’s medium needs 2-5% FBS and provides more consistency.
Description
Advanced Glasgow’s Modified Eagle’s Medium
Background
PurMaTM Advanced Glasgow’s Modified Eagle’s Medium is free of glutamine amino acids. Moreover, it contains components that allow for a 60–90% reduction in FBS supplementation. More importantly, using synthetic components such as recombinant insulin, albumin, and transferrin increase consistency. This results in less variation amongst lot-to-lot serum changes. In A-GMEM, L-Alanyl-L-Glutamine is used as the source of glutamine. Additionally, reducing FBS requirements lowers the cost of cell culture experiments.
Contents
Advanced Glasgow’s Contains:
- includes all twenty essential and non-essential amino acids with higher concentrations than regular and MAX formulations.
- A-GMEM incorporates elements that significantly reduce the need for a serum for cell culture.
Crucial Ingredients:
- PurMaTM Human Transferrin (Holo): prevents Improper iron storage and delivery in cell culture systems which is a major contributor to oxidative stress and protein damage.
- PurMaTM Insulin Recombinant
- Trace of other elements which mimic the elements in fetal bovine serum.
Consequently, Advanced Glasgow’s Modified Eagle’s Medium is a significant step toward a chemically defined form of this media. Unquestionably, Chemically Defined Glasgow’s medium (CD-Glasgow’s meium) is the outcome of decades of cutting-edge research and will soon be offered globally to laboratories that are looking for consistency and repeatability in their data.
Formulation
Complete formulation is available here: Advanced Glasgow’s Formulation
References
- The stimulation of Na+, K+-ATPase activity in the medulla of the rat kidney by [arginine] vasopressin and its analogues. Pippard C, Baylis PH.Clin Sci (Lond). 1984 May;66(5):561-7. doi: 10.1042/cs0660561.PMID: 10.1023/a:1017989305873. PMID: 15348233.
- Induction of dopamine-releasing cells from primate embryonic stem cells enclosed in agarose microcapsules. Ando T, Yamazoe H, Moriyasu K, Ueda Y, Iwata H.Tissue Eng. 2007 Oct;13(10):2539-47. doi: 10.1089/ten.2007.0045.PMID:
- Uptake of glutamate, not glutamine synthetase, regulates adaptation of mammalian cells to glutamine-free medium. McDermott RH, Butler M.J Cell Sci. 1993 Jan;104 ( Pt 1):51-8. doi: 10.1242/jcs.104.1.51.PMID:
| Parameter | Specification |
|---|---|
| Appearance | Red, clear liquid |
| pH | 7.2 ± 0.1 |
| Osmolality | 275-360 mOsm/L |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Suitability | Suitable for mammalian cell culture |
| Additive | Sodium pyruvate |
| Indicator | Phenol red |
| Mycoplasma Detection | Negative |
| Sterility Tested | Sterile filtered using 0.22 µm filter |
| Form | Liquid |
| Shipping Condition | Room temperature |
Additional information
| Condition | A-GMEM Standard Formulation, A-GMEM w/o Phenol Red, A-GMEM w/o Sodium Bicarbonate, A-GMEM w/o Sodium Pyruvate, A-GMEM w/o Glucose, A-GMEM w/o Phenol Red, w/o Sodium Bicarbonate, A-GMEM w/o Sodium Bicarbonate, w/o Sodium Pyruvate, A-GMEM w/o Phenol Red, w/o Sodium Bicarbonate, w/o Sodium Pyruvate, A-GMEM Low Glucose (1.5 g/L), A-GMEM Low Sodium Bicarbonate (0.35 g/L), A-GMEM Low Sodium Bicarbonate (1.1 g/L), A-GMEM 15 mM HEPES (3.6 g/L), A-GMEM 25 mM HEPES (5.9 g/L), A-GMEM 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.1 g/L), A-GMEM 25 mM HEPES (5.9 g/L), Low Sodium Bicarbonate (1.1 g/L) |
|---|---|
| Format | Liquid, Powder |
| Size | 1 X 10L ( Powder), 1 X 50 L (Powder), 10 X 1EZ Individually Packed (Powder), 50 X 1EZ Individually Packed (Powder), 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml |
Quality Control
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