Advanced Glasgow’s Modified Eagle’s Medium is free of glutamine contains L-Alanyl-L-Glutamine as well as ingredients to allow for serum reduction. Additionally, Advanced Glasgow’s medium needs 2-5% FBS and provides more consistency.
Advanced Glasgow’s Modified Eagle’s Medium
$49.10 – $907.05
Description
Advanced Glasgow’s Modified Eagle’s Medium
Background
PurMaTM Advanced Glasgow’s Modified Eagle’s Medium is free of glutamine amino acids. Moreover, it contains components that allow for a 60–90% reduction in FBS supplementation. More importantly, using synthetic components such as recombinant insulin, albumin, and transferrin increase consistency. This results in less variation amongst lot-to-lot serum changes. In A-GMEM, L-Alanyl-L-Glutamine is used as the source of glutamine. Additionally, reducing FBS requirements lowers the cost of cell culture experiments.
Contents
Advanced Glasgow’s Contains:
- includes all twenty essential and non-essential amino acids with higher concentrations than regular and MAX formulations.
- A-GMEM incorporates elements that significantly reduce the need for a serum for cell culture.
Crucial Ingredients:
- PurMaTM Human Transferrin (Holo): prevents Improper iron storage and delivery in cell culture systems which is a major contributor to oxidative stress and protein damage.
- PurMaTM Insulin Recombinant
- Trace of other elements which mimic the elements in fetal bovine serum.
Consequently, Advanced Glasgow’s Modified Eagle’s Medium is a significant step toward a chemically defined form of this media. Unquestionably, Chemically Defined Glasgow’s medium (CD-Glasgow’s meium) is the outcome of decades of cutting-edge research and will soon be offered globally to laboratories that are looking for consistency and repeatability in their data.
Formulation
Complete formulation is available here: Advanced Glasgow’s Formulation
References
- The stimulation of Na+, K+-ATPase activity in the medulla of the rat kidney by [arginine] vasopressin and its analogues. Pippard C, Baylis PH.Clin Sci (Lond). 1984 May;66(5):561-7. doi: 10.1042/cs0660561.PMID: 10.1023/a:1017989305873. PMID: 15348233.
- Induction of dopamine-releasing cells from primate embryonic stem cells enclosed in agarose microcapsules. Ando T, Yamazoe H, Moriyasu K, Ueda Y, Iwata H.Tissue Eng. 2007 Oct;13(10):2539-47. doi: 10.1089/ten.2007.0045.PMID:
- Uptake of glutamate, not glutamine synthetase, regulates adaptation of mammalian cells to glutamine-free medium. McDermott RH, Butler M.J Cell Sci. 1993 Jan;104 ( Pt 1):51-8. doi: 10.1242/jcs.104.1.51.PMID:
Parameter | Specification |
---|---|
Appearance | Red, clear liquid |
pH | 7.2 ± 0.1 |
Osmolality | 275-360 mOsm/L |
Endotoxin | NMT< 2EU/mL |
Mycoplasma | Negative |
Suitability | Suitable for mammalian cell culture |
Additive | Sodium pyruvate |
Indicator | Phenol red |
Mycoplasma Detection | Negative |
Sterility Tested | Sterile filtered using 0.22 µm filter |
Form | Liquid |
Shipping Condition | Room temperature |
Quality Control
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Iscove’s Modified Dulbecco’s Medium MAX (IMDM-MAX)
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filter_sepia_hover="0" filter_opacity_hover="100" filter_blur_hover="0"][fusion_builder_row][fusion_builder_column type="1_1" layout="1_1" align_self="auto" content_layout="column" align_content="flex-start" valign_content="flex-start" content_wrap="wrap" spacing="" center_content="no" column_tag="div" link="" target="_self" link_description="" min_height="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" sticky_display="normal,sticky" class="" id="" type_medium="" type_small="" order_medium="0" order_small="0" dimension_spacing_medium="" dimension_spacing_small="" dimension_spacing="" dimension_margin_medium="" dimension_margin_small="" margin_top="" margin_bottom="" padding_medium="" padding_small="" padding_top="" padding_right="" padding_bottom="" padding_left="" hover_type="none" border_sizes="" border_color="" border_style="solid" border_radius="" box_shadow="no" dimension_box_shadow="" box_shadow_blur="0" box_shadow_spread="0" box_shadow_color="" box_shadow_style="" z_index_subgroup="regular" z_index="" z_index_hover="" overflow="" background_type="single" gradient_start_color="" gradient_end_color="" gradient_start_position="0" gradient_end_position="100" gradient_type="linear" radial_direction="center center" linear_angle="180" background_color="" background_image="" background_image_id="" lazy_load="none" skip_lazy_load="" background_position="left top" background_repeat="no-repeat" background_blend_mode="none" render_logics="" sticky="off" sticky_devices="small-visibility,medium-visibility,large-visibility" sticky_offset="" absolute="off" absolute_props="" filter_type="regular" filter_hover_element="self" filter_hue="0" filter_saturation="100" filter_brightness="100" filter_contrast="100" filter_invert="0" filter_sepia="0" filter_opacity="100" filter_blur="0" filter_hue_hover="0" filter_saturation_hover="100" filter_brightness_hover="100" filter_contrast_hover="100" filter_invert_hover="0" filter_sepia_hover="0" filter_opacity_hover="100" filter_blur_hover="0" transform_type="regular" transform_hover_element="self" transform_scale_x="1" transform_scale_y="1" transform_translate_x="0" transform_translate_y="0" transform_rotate="0" transform_skew_x="0" transform_skew_y="0" transform_scale_x_hover="1" transform_scale_y_hover="1" transform_translate_x_hover="0" transform_translate_y_hover="0" transform_rotate_hover="0" transform_skew_x_hover="0" transform_skew_y_hover="0" transform_origin="" transition_duration="300" transition_easing="ease" transition_custom_easing="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset="" last="true" border_position="all" first="true"][fusion_text columns="" column_min_width="" column_spacing="" rule_style="" rule_size="" rule_color="" hue="" saturation="" lightness="" alpha="" content_alignment_medium="" content_alignment_small="" content_alignment="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" sticky_display="normal,sticky" class="" id="" margin_top="" margin_right="" margin_bottom="" margin_left="" fusion_font_family_text_font="" fusion_font_variant_text_font="" font_size="" line_height="" letter_spacing="" text_transform="" text_color="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset=""]Iscove’s Modified Dulbecco’s Medium MAX (IMDM-MAX)
Background
Iscove’s Modified Dulbecco’s Medium MAX (IMDM-MAX) is a commonly used media for Mammalian cell culture.Advantages
The advantages of IMDM-MAX over IMDM are:
- Higher PH Capacity
- Moreover, it contains 4 mM L-Alanyl-L-Glutamine (0.869 g/L) instead of L-Glutamine and therefore:
- Possess longer shelf life.
- More importantly, minimizes toxic ammonia build-up.
- Significantly improves cell viability and growth.
- Remains stable across a wide range of temperatures.
- Improves the culture of primary cells.
Mechanism
Although L-glutamine is a vital amino acid, it degrades, resulting in the generation of toxic ammonia and pyrrolidine carboxylic acid. One way to minimize L-glutamine degradation in media is to gradually add these amino acids to your cell culture. However, keeping track of time to keep the amount of L-glutamine at the same level is tedious, if not impossible. Alternatively, L-alanyl-L-glutamine can be used because it is much more stable in aqueous solutions. More importantly, it does not easily degrade and gradually releases aminopeptidases. As a result, L-glutamine and L-alanine are then used by the cells for protein production in the TCA cycle. In addition to our Max formulation products, we manufacture PurMaTM GluaSup (Cat# P3S210 559) is a combination of L-alanyl-L-glutamine which prevents degradation of L-glutamine.Contents
Iscove’s Modified Dulbecco’s Medium MAX (IMDM-MAX) contains:
- 5 mM sodium pyruvate
- High glucose (4.5g/L)
- Non-Essential Amino Acids
- 3.7 g/L Sodium Bicarbonate; PurMa Biologics Manufactures several types of IMDM-MAX based on the amount of Sodium Bicarbonate and HEPES (PH capacity)
- Phenol Red
Formulation
Complete formulation click here: IMDM-MAX FormulationReferences
- Plaque production by the polyoma virus. DULBECCO R, FREEMAN G. 1959 Jul;8(3):396-7. doi: 10.1016/0042-6822(59)90043-1. PMID: 13669362.
- Complete replacement of serum by albumin, transferrin, and soybean lipid in cultures of lipopolysaccharide-reactive B lymphocytes. J Exp Med. 1978 Mar 1; 147(3): 923–933. doi: 10.1084/jem.147.3.923 PMCID: PMC2184195
- Development of an infusible-grade solution for non-cryopreserved hematopoietic cell storage. Burger SR, Hubel A, McCullough J. 1999;1(2):123-33. doi:10.1080/0032472031000141250. PMID: 19746589
Parameter Specification Appearance Red, clear liquid pH 7.2 ± 0.1 Osmolality 275-360 mOsm/L Endotoxin NMT< 2EU/mL Mycoplasma Negative Suitability Suitable for mammalian cell culture Additive Sodium pyruvate Indicator Phenol red Mycoplasma Detection Negative Sterility Tested Sterile filtered using 0.22 µm filter Form Liquid Shipping Condition Room temperature Advanced Leibovitz’s L-15 Medium
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filter_sepia_hover="0" filter_opacity_hover="100" filter_blur_hover="0"][fusion_builder_row][fusion_builder_column type="1_1" layout="1_1" align_self="auto" content_layout="column" align_content="flex-start" valign_content="flex-start" content_wrap="wrap" spacing="" center_content="no" column_tag="div" link="" target="_self" link_description="" min_height="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" sticky_display="normal,sticky" class="" id="" type_medium="" type_small="" order_medium="0" order_small="0" dimension_spacing_medium="" dimension_spacing_small="" dimension_spacing="" dimension_margin_medium="" dimension_margin_small="" margin_top="" margin_bottom="" padding_medium="" padding_small="" padding_top="" padding_right="" padding_bottom="" padding_left="" hover_type="none" border_sizes="" border_color="" border_style="solid" border_radius="" box_shadow="no" dimension_box_shadow="" box_shadow_blur="0" box_shadow_spread="0" box_shadow_color="" box_shadow_style="" z_index_subgroup="regular" z_index="" z_index_hover="" overflow="" background_type="single" gradient_start_color="" gradient_end_color="" gradient_start_position="0" gradient_end_position="100" gradient_type="linear" radial_direction="center center" linear_angle="180" background_color="" background_image="" background_image_id="" lazy_load="none" skip_lazy_load="" background_position="left top" background_repeat="no-repeat" background_blend_mode="none" render_logics="" sticky="off" sticky_devices="small-visibility,medium-visibility,large-visibility" sticky_offset="" absolute="off" absolute_props="" filter_type="regular" filter_hover_element="self" filter_hue="0" filter_saturation="100" filter_brightness="100" filter_contrast="100" filter_invert="0" filter_sepia="0" filter_opacity="100" filter_blur="0" filter_hue_hover="0" filter_saturation_hover="100" filter_brightness_hover="100" filter_contrast_hover="100" filter_invert_hover="0" filter_sepia_hover="0" filter_opacity_hover="100" filter_blur_hover="0" transform_type="regular" transform_hover_element="self" transform_scale_x="1" transform_scale_y="1" transform_translate_x="0" transform_translate_y="0" transform_rotate="0" transform_skew_x="0" transform_skew_y="0" transform_scale_x_hover="1" transform_scale_y_hover="1" transform_translate_x_hover="0" transform_translate_y_hover="0" transform_rotate_hover="0" transform_skew_x_hover="0" transform_skew_y_hover="0" transform_origin="" transition_duration="300" transition_easing="ease" transition_custom_easing="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset="" last="true" border_position="all" first="true"][fusion_text columns="" column_min_width="" column_spacing="" rule_style="" rule_size="" rule_color="" hue="" saturation="" lightness="" alpha="" content_alignment_medium="" content_alignment_small="" content_alignment="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" sticky_display="normal,sticky" class="" id="" margin_top="" margin_right="" margin_bottom="" margin_left="" fusion_font_family_text_font="" fusion_font_variant_text_font="" font_size="" line_height="" letter_spacing="" text_transform="" text_color="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset=""]Advanced Leibovitz’s L-15 Medium
Background
PurMaTM Advanced Leibovitz’s L-15 Medium contains components that allow for a 60–90% reduction in FBS supplementation. More importantly, using synthetic components such as recombinant insulin, albumin, and transferrin increases consistency. This results in less variation amongst lot-to-lot serum changes. In A-Leibovitz’s L-15, L-Alanyl-L-Glutamine is used as the source of glutamine. Additionally, reducing FBS requirements lowers the cost of cell culture experiments.Contents
Advanced Leibovitz’s L-15 Contains:
- includes all twenty essential and non-essential amino acids with higher concentrations than regular and MAX formulations.
- incorporates elements that significantly reduce the need for a serum for cell culture.
Crucial Ingredients:
- PurMaTM Human Transferrin (Holo): prevents Improper iron storage and delivery in cell culture systems which is a major contributor to oxidative stress and protein damage.
- PurMaTM Insulin Recombinant
- Trace of other elements which mimic the elements in fetal bovine serum.
Formulation
Complete formulation is available here: Advanced Leibovitz's L-15 FormulationReferences
- The effect of a phorbol ester on amphibian myogenesis. Zeng MB, et al. 1990 Mar;23(1):106-15. PMID: 2382522.
- Cold inhibits neurite outgrowth from single retinal ganglion cells isolated from adult goldfish. Ishida AT, Cheng MH.Exp Eye Res. 1991 Feb;52(2):175-91. doi: 10.1016/0014-4835(91)90257-f.PMID: 2013300
Parameter Specification Appearance Red, clear liquid pH 7.2 ± 0.1 Osmolality 275-360 mOsm/L Endotoxin NMT< 2EU/mL Mycoplasma Negative Suitability Suitable for mammalian cell culture Additive Sodium pyruvate Indicator Phenol red Mycoplasma Detection Negative Sterility Tested Sterile filtered using 0.22 µm filter Form Liquid Shipping Condition Room temperature Basal Medium Eagle-MAX (BME-MAX) in Hanks’ Buffer
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hide_on_mobile="small-visibility,medium-visibility,large-visibility" sticky_display="normal,sticky" class="" id="" margin_top="" margin_right="" margin_bottom="" margin_left="" fusion_font_family_text_font="" fusion_font_variant_text_font="" font_size="" line_height="" letter_spacing="" text_transform="" text_color="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset=""]Basal Medium Eagle-MAX (BME-MAX) in Hanks’ Buffer
Background
Basal Medium Eagle-MAX (BME-MAX) in Hanks’ Buffer is a commonly used media for Mammalian cell culture.Advantages
The advantages of BME-MAX over BME are:
- Higher PH Capacity
- Moreover, it contains 4 mM L-Alanyl-L-Glutamine (0.869 g/L) instead of L-Glutamine and therefore:
- Possess longer shelf life.
- More importantly, minimizes toxic ammonia build-up.
- Significantly improves cell viability and growth.
- Remains stable across a wide range of temperatures.
- Improves the culture of primary cells.
Mechanism
Although L-glutamine is a vital amino acid, it degrades, generating toxic ammonia and pyrrolidine carboxylic acid. One way to minimize L-glutamine degradation in media is to gradually add these amino acids to your cell culture. However, keeping track of time to keep the amount of L-glutamine at the same level is tedious, if not impossible. Alternatively, L-alanyl-L-glutamine can be used because it is much more stable in aqueous solutions. More importantly, it does not easily degrade and gradually releases aminopeptidases. As a result, L-glutamine and L-alanine are then used by the cells for protein production in the TCA cycle. In addition to our Max formulation products, we manufacture PurMaTM GluaSup (Cat# P3S210 559) is a combination of L-alanyl-L-glutamine that prevents the degradation of L-glutamine.Contents
Basal Medium Eagle-MAX (BME-MAX) in Hanks’ Buffer contains:
- 5 mM sodium pyruvate
- High glucose (4.5g/L)
- Non-Essential Amino Acids
- 3.7 g/L Sodium Bicarbonate; PurMa Biologics Manufactures several types of BME-MAX in HBBS based on the amount of Sodium Bicarbonate and HEPES (PH capacity)
- Phenol Red
Formulation
The complete formulation can be found here: BME-MAX with Hanks' Buffer (HBSS) FormulationReferences
- Improved chemically defined basal medium (CMRL-1969) for primary monkey kidney and human diploid cells. Healy GM, et al. Appl Microbiol. 1971 Jan;21(1):1-5. doi: 10.1128/am.21.1.1-5.1971. PMID:
- Nutritional requirements for the production of herpes simplex virus. I. Influence of glucose and glutamine of herpes simplex virus production by HeLa cells. LEWIS VJ Jr, SCOTT LV. J Bacteriol. 1962 Mar;83(3):475-82. doi: 10.1128/jb.83.3.475-482.1962. PMID: 14464909
- An improved culture system for secondary palatal elevation. Lewis CA, Thibault L, Pratt RM, Brinkley LL. In Vitro. 1980 Jun;16(6):453-60. doi: 10.1007/BF02626457. PMID: 7390537
Parameter Specification Appearance Red, clear liquid pH 7.2 ± 0.1 Osmolality 275-360 mOsm/L Endotoxin NMT< 2EU/mL Mycoplasma Negative Suitability Suitable for mammalian cell culture Additive N/A Indicator Phenol red Mycoplasma Detection Negative Sterility Tested Sterile filtered using 0.22 µm filter Form Liquid Shipping Condition Room temperature Kaighn’s Modification of Ham’s F-12-K Medium
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filter_opacity_hover="100" filter_blur_hover="0" transform_type="regular" transform_hover_element="self" transform_scale_x="1" transform_scale_y="1" transform_translate_x="0" transform_translate_y="0" transform_rotate="0" transform_skew_x="0" transform_skew_y="0" transform_scale_x_hover="1" transform_scale_y_hover="1" transform_translate_x_hover="0" transform_translate_y_hover="0" transform_rotate_hover="0" transform_skew_x_hover="0" transform_skew_y_hover="0" transform_origin="" transition_duration="300" transition_easing="ease" transition_custom_easing="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset="" last="true" border_position="all" first="true"][fusion_text columns="" column_min_width="" column_spacing="" rule_style="" rule_size="" rule_color="" hue="" saturation="" lightness="" alpha="" content_alignment_medium="" content_alignment_small="" content_alignment="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" sticky_display="normal,sticky" class="" id="" margin_top="" margin_right="" margin_bottom="" margin_left="" fusion_font_family_text_font="" fusion_font_variant_text_font="" font_size="" line_height="" letter_spacing="" text_transform="" text_color="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset=""]Kaighn's Modification of Ham's F-12-K Medium
Background
Kaighn's Modification of Ham's F-12-K Medium is a modified version of Ham’s F-12 medium. originally, Ham’s F-12K (Kaighn’s) was used for primary cells such as human oocytes and hepatocytes, as well murine and chicken liver cells in a lower serum environment. but, however, recently this media is used for a much larger variety of cells. Moreover, Ham’s F-12K (Kaighn’s) Medium contains a variety of components not found in older media, such as putrescine thymidine, hypoxanthine, zinc, and higher levels of all amino acids and sodium pyruvate.Ingredients:
F-12K Medium also contains:
– Low level of glucose (1.26 g/ liter) – Sodium bicarbonate (2.5 g/L) (if higher than 5% CO2 is used, a higher % of sodium bicarbonate is needed). – Additionally, PurMa Biologics also offers F-12K Medium with different level of sodium bicarbonate : 1.5 g/L (Cat # P3S057108) as well as, 3.7g/L (Cat # P3S032108) – 2 mM L-glutamine (292 mg/L)’ – A higher concentration of amino acids, vitamins, inositol and choline are present in this medium.Why PurMa Biologics? Why we stand behind our Cell Culture Media:
Our products are the outcome of 30 years of experience in cutting edge cell and tissue culture science. Also, we utilize %99 pure amino acids. This will significantly increase the consistency among your experiments, and the result of your lab compared to other laboratories working on the same issues. We routinely manufacture over 1500 media, which is the most comprehensive collection of cell and tissue culture media. Considering the highest quality, our prices are extremely reasonable.Application of Ham’s F-12K:
This media has elements that prevent clumping, therefore, it is ideal for culturing primary as well as commercial cell lines, for instance normal and neoplastic leukocytes, HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, carcinomas, bone marrow, and hybridoma cells.Formulation:
For complete formulation click here: Ham's F-12K FormulationReferences:
- Growth and continuous passage of COMMA-D mouse mammary epithelial cells in hormonally defined serum-free medium. Riss et al. Cancer Res. 1987 Jul 15;47(14):3776-82. PMID: 3297308.
- Human recombinant insulin-like growth factor I. I. Development of a serum-free medium for clonal density assay of growth factors using BALB/c 3T3 mouse embryo fibroblasts. Riss et al. In Vitro Cell Dev Biol. 1988 Nov;24(11):1099-106. doi: 10.1007/BF02620811.
- Hormones and factors that stimulate growth of a rat islet tumor cell line in serum-free medium. Fong et al. Diabetes. 1981 Dec;30(12):1022-8. doi: 10.2337/diab.30.12.1022. PMID: 6273246
Parameter Specification Appearance Red, clear liquid pH 7.2 ± 0.1 Osmolality 275-360 mOsm/L Endotoxin NMT< 2EU/mL Mycoplasma Negative Suitability Suitable for mammalian cell culture Additive Sodium pyruvate Indicator Phenol red Mycoplasma Detection Negative Sterility Tested Sterile filtered using 0.22 µm filter Form Liquid Shipping Condition Room temperature Title
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