PurMa™ Dulbecco’s Modified Eagle’s Medium/F-12 (DMEM F-12)
A high-performance DMEM/F-12 formulation engineered for consistent mammalian cell culture.
- Enriched with 2.5 mM L-glutamine, 0.5 mM sodium pyruvate, 30 mM NaHCO3, high glucose, non-essential amino acids, and phenol red
- Ideal for MDCK, glial cells, fibroblasts, and human endothelial cultures
- ✓Proudly made in the USA, manufactured in house
- ✓Certificate of Analysis available for every lot
- ✓Backed by decades of cell culture and protein biochemistry expertise
- ✓Bulk pricing, purchase orders, and custom formulations welcome
Description
PurMa™ Dulbecco’s Modified Eagle’s Medium/F-12 (DMEM F-12)
Background
PurMa™ Dulbecco’s Modified Eagle’s Medium/F-12 (DMEM F-12) is an enriched DMEM/F‑12 formulation developed for reliable, reproducible mammalian cell culture and optimized for cell types that benefit from additional nutrients and buffering. This advanced DMEM F‑12 supports MDCK, glial cells, fibroblasts, human endothelial cells and rat fibroblasts, providing consistent performance in research and production workflows.
Special Features
- L‑Glutamine: 2.5 mM (stabilized where applicable)
- Sodium pyruvate: 0.5 mM
- Sodium bicarbonate (NaHCO3): 30 mM (2.438 g/L) for robust CO2 buffering
- High glucose level for energy‑intensive cell types
- Non‑essential amino acids to support protein synthesis and growth
- Phenol red pH indicator for culture monitoring
Why choose PurMa Advanced DMEM F‑12 (DMEM F‑12 formulation advantages)
- Consistency: Manufactured under stringent QC for lot‑to‑lot uniformity.
- Moreover, enriched composition of advanced DMEM F‑12: Compared with standard DMEM, DMEM F‑12 provides additional pyruvate, amino acids and optimized buffering—see “DMEM f12 vs DMEM” for differences in nutrient profile and application.
- Versatility: Well‑suited for a broad range of adherent and suspension mammalian cell lines that demand enriched media.
- Ready for supplementation: Compatible with serum, defined supplements, growth factors, and antibiotics per your protocol.
Applications
- Advanced Dulbecco’s Modified Eagle’s Medium/F-12 (DMEM F-12) is suitable for MDCK cell culture
- Glial and neural cell maintenance
- Primary and immortalized fibroblast culture
- Human endothelial cell expansion
- Routine cell maintenance, proliferation assays, and transfection workflows
Handling & Storage
- Store Advanced Dulbecco’s Modified Eagle’s Medium/F-12 (DMEM F-12) unopened at 2–8 °C protected from light.
- Warm to 37 °C before use and handle aseptically.
- Use prepared Advanced DMEM F‑12 according to your lab SOPs; supplement with serum or additives as required.
Quality & Support Dulbecco’s Modified Eagle’s Medium/F-12 (DMEM F-12) is produced in a controlled manufacturing environment with full documentation and Certificate of Analysis available for each lot. Contact technical support for media optimization and supplementation guidance.
Formulation
For complete formulation click here which is identical to thermofisher and gibco advanced dmemf12: DMEM F-12 Formulation
References:
- Clonal growth of mammalian cells in a chemically defined, synthetic medium . DULBECCO et al. 1959 Jul;8(3):396-7. doi: 10.1016 /0042-68 22(59)90043-1. PMID: 13669362.
- Plaque production by the polyoma virus. DULBECCO et al. 1959 Jul;8(3):396-7. doi: 10.1016/0042-6822(59)90043-1. PMID: 1366936.
| Parameter | Specification |
|---|---|
| Appearance | Red, clear liquid |
| pH | 7.2 ± 0.1 |
| Osmolality | 275-360 mOsm/L |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Suitability | Suitable for mammalian cell culture |
| Additive | Sodium pyruvate |
| Indicator | Phenol red |
| Mycoplasma Detection | Negative |
| Sterility Tested | Sterile filtered using 0.22 µm filter |
| Form | Liquid |
| Shipping Condition | Room temperature |
Learn more: What Is Ham’s F-12K?
Additional information
| Condition | DMEM F-12 Standard Formulation, DMEM F-12 Standard Formulation, ATCC Formulation, DMEM F-12 w/o Glutamine, DMEM F-12 w/o Phenol Red, DMEM F-12 w/o Sodium Bicarbonate, DMEM F-12 w/o Sodium Pyruvate, DMEM F-12 w/o Glucose, DMEM F-12 w/o Lipoic Acid, DMEM F-12 w/o Glutamine, w/o Phenol Red, DMEM F-12 w/o Phenol Red, w/o Sodium Bicarbonate, DMEM F-12 w/o Sodium Bicarbonate, w/o Sodium Pyruvate, DMEM F-12 w/o Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, DMEM F-12 w/o Phenol Red, w/o Sodium Bicarbonate, w/o Sodium Pyruvate, DMEM F-12 Low Glucose (1.5 g/L), DMEM F-12 Low Sodium Bicarbonate (1.2 g/L), DMEM F-12 15 mM HEPES (3.6 g/L), DMEM F-12 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.2 g/L), DMEM F-12 w/o Sodium Pyruvate, w/o phenol Red, DMEM F-12 w/o Phenol Red, with 15 mM HEPES (3.6 g/L), DMEM F-12 w/o Phenol Red, Low Sodium Bicarbonate (1.2 g/L), DMEM F-12 w/o Sodium Pyruvate, Low Sodium Bicarbonate (1.2 g/L), DMEM F-12 High Glucose (4.5 g/L) |
|---|---|
| Format | Liquid, Powder |
| Size | 10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L |
Documents
Certificate of Analysis (COA): Available upon request for your specific order — email info@purmabiologics.com and we will send the current lot documentation.
Safety Data Sheet (SDS): Available upon request — email info@purmabiologics.com.
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