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Dulbecco′s Modified Eagle′s Medium/F-12 MAX (DMEM F12-MAX)

Background:

Dulbecco′s Modified Eagle′s Medium/F-12 MAX (DMEM F12-MAX) is a commonly used media for Mammalian cell culture.

Advantages:

DMEM F-12 MAX over DMEM:

• Higher PH Capacity
• Moreover, it contains 4 mM L-Alanyl-L-Glutamine (0.869 g/L) instead of L-Glutamine and therefore:
• Possess longer shelf life.
• More importantly, minimizes toxic ammonia build-up.
• Significantly improves cell viability and growth.
• Remains stable across a wide range of temperatures.
• Improves the culture of primary cells.

Mechanism:

Although L-glutamine is a vital amino acid, it degrades, resulting in the generation of toxic ammonia and pyrrolidine carboxylic acid. One way to minimize L-glutamine degradation in media is to gradually add these amino acids to your cell culture. However, keeping track of time to keep the amount of L-glutamine at the same level is tedious, if not impossible. Alternatively, L-alanyl-L-glutamine can be used because it is much more stable in aqueous solutions. More importantly, it does not easily degrade and gradually releases aminopeptidases. As a result, L-glutamine and L-alanine are then used by the cells for protein production in the TCA cycle. In addition to our Max formulation products, we manufacture PurMaTM GluaSup (Cat# P3S210 559) is a combination of L-alanyl-L-glutamine which prevents degradation of L-glutamine.

Contents:

• Dulbecco′s Modified Eagle′s Medium/F-12 MAX (DMEM F12-MAX) contains:
• 5 mM sodium pyruvate
• High glucose (4.5g/L)
• Non-Essential Amino Acids
• 3.7 g/L Sodium Bicarbonate; PurMa Biologics Manufactures several types of DMEM F12-MAX based on the amount of Sodium Bicarbonate and HEPES (PH capacity)
• Phenol Red

DMEM F12-MAX contains no proteins, lipids, or growth factors. Therefore, it requires supplementation, commonly with 10% Fetal bovine Serum (FBS).

Formulation:

Complete formulation is here: DMEM F-12 MAX Formulation

References:

1.  Clonal growth of mammalian cells in a chemically defined, synthetic medium . DULBECCO R, FREEMAN G. 1959 Jul;8(3):396-7. doi: 10.1016/0042-6822(59)90043-1. PMID: 13669362.
2. Plaque production by the polyoma virus. DULBECCO R, FREEMAN G. 1959 Jul;8(3):396-7. doi: 10.1016/0042-6822(59)90043-1. PMID: 13669362.
3. The nucleic acid of polyoma virus. Smith, J.D., Freeman, G., Vogt, M., et al., (1960) The nucleic acid of polyoma virus. Virology 12:18

[/fusion_text][fusion_table fusion_table_type="1" fusion_table_rows="13" fusion_table_columns="2" margin_top="30px" margin_right="" margin_bottom="" margin_left="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" class="" id="" animation_type="" animation_direction="left" animation_color="" hue="" saturation="" lightness="" alpha="" animation_speed="0.3" animation_delay="0" animation_offset=""] [/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed Read more