DMEM-F-12 (Dulbecco’s Modified Eagle Medium Nutrient-Mixture F-12) provides superior quality, consistency, and great control in mammalian cell culture.
This formulation combines DMEM’s high concentrations of glucose, amino acids, and vitamins as well as much more variation of salts with DMEM-F-12 ’s wide variety of components.
This media is better than DMEM and advanced IMDM on MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. It contains no proteins, lipids, or growth factors. Therefore, it requires supplementation, commonly with 10% Fetal Bovine Serum (FBS).
In general, DMEM- F-12 is more enriched but contains the following major changes compared to DMEM:
- 2.5 mM L-glutamine,
- 0.5 mM sodium pyruvate,
- 30mM NaHCO3 (2.438g/L)
High glucose, - Non-Essential Amino Acids,
- Sodium pyruvate,
- Phenol Red
- L-glutamine
In some formulations, the concentration of sodium bicarbonate used is lower than DMEM, therefore requires a 5–10% CO2 environment to maintain physiological pH.
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- DMEM F-12, Cell Culture Media
Advanced Dulbecco’s Modified Eagle Medium/F-12 (A-DMEM F-12)
- Advanced Dulbecco's Modified Eagle Medium/F-12 (A-DMEM F-12) needs 2-5% FBS and provides more consistency.
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- DMEM F-12, Cell Culture Media
Dulbecco’s Modified Eagle’s Medium/F-12 (DMEM F-12)
- Dulbecco’s Modified Eagle’s Medium/F-12 (DMEM F-12) is commonly used worldwide. Moreover, DMEM F-12 contains more nutrition and offers more buffering capacity. Therefore, this medium provides more growth and maintenance ability.
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- DMEM F-12, Cell Culture Media
Dulbecco′s Modified Eagle′s Medium/F-12 MAX (DMEM F12-MAX)
- Dulbecco′s Modified Eagle′s Medium/F-12 MAX (DMEM F12-MAX) contains L-Alanyl-L-Glutamine, therefore supplying a longer shelf life. DMEM F12-MAX significantly improves cell viability and growth. We manufacture several types of DMEM F12-MAX based on your requirements.
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