DMEM-F-12

(Dulbecco’s Modified Eagle Medium Nutrient-Mixture F-12) provides superior quality, consistency, and great control in mammalian cell culture.

This formulation combines DMEM’s high concentrations of glucose, amino acids, and vitamins as well as much more variation of salts with DMEM-F-12 ’s wide variety of components.

This media is better than DMEM and advanced IMDM on MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. It contains no proteins, lipids, or growth factors. Therefore, it requires supplementation, commonly with 10% Fetal Bovine Serum (FBS).

In general, DMEM- F-12 is more enriched but contains the following major changes compared to DMEM:

  • 2.5 mM L-glutamine,
  • 0.5 mM sodium pyruvate,
  • 30mM NaHCO3 (2.438g/L)
    High glucose,
  • Non-Essential Amino Acids,
  • Sodium pyruvate,
  • Phenol Red
  • L-glutamine

In some formulations, the concentration of sodium bicarbonate used is lower than DMEM, therefore requires a 5–10% CO2 environment to maintain physiological pH.

  • PurMa™ Chemically Defined DMEM/F12 (CD-DMEM F-12) Quick View
    • PurMa™ Chemically Defined DMEM/F12 (CD-DMEM F-12) Quick View
    • Cell Culture Media, DMEM F-12

    • PurMa™ Chemically Defined DMEM/F12 (CD-DMEM F-12)

    • PurMa™ Chemically Defined DMEM/F12 is a serum-free, chemically defined DMEM/F12 medium containing L-alanyl-L-glutamine and a proprietary mix of synthetic growth factors. This chemically defined DMEM/F12 provides a fully controlled, animal-component–free environment, enabling reproducible mammalian cell culture, easier regulatory compliance, and a smooth, step wise transition from FIBS-containing media.
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  • PurMa™ Dulbecco’s Modified Eagle’s Medium/F-12 MAX (DMEM F12-MAX) Quick View
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      PurMa™ Dulbecco’s Modified Eagle’s Medium/F-12 MAX (DMEM F12-MAX) Quick View
    • Cell Culture Media, DMEM F-12

    • PurMa™ Dulbecco’s Modified Eagle’s Medium/F-12 MAX (DMEM F12-MAX)

    • Price range: $31.10 through $590.46
    • PurMa™ DMEM-MAX with L-alanyl-L-glutamine  is a high‑performance Dulbecco’s Modified Eagle’s Medium/F‑12 supplemented with 4 mM L‑alanyl‑L‑glutamine, offering higher pH buffering capacity, longer shelf life, reduced ammonia buildup, and improved viability and growth of mammalian and primary cell.more over, DMEM-MAX with L-alanyl-L-glutamine is suitable for a wide range of cell lines.
    • Select options This product has multiple variants. The options may be chosen on the product page