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- ✓Bulk pricing, purchase orders, and custom formulations welcome
Chemically Defined Iscove’s Modified Dulbecco’s Medium (CD-IMDM)
PurMa Biologics is the first company has generated Chemically Defined IMDM which does not need FBS and does not contain any animal products. This provides consistency and reproducibility of your data.
Description
Background
PurMaTM Chemically Defined Iscove’s Modified Dulbecco’s Medium (CD-IMDM) is a proprietary formulation used to grow cells in vitro without any undefined or animal-derived components. Moreover, CD-IMDM contains known quantities of pure chemicals, including amino acids, vitamins, salts, sugars, and more importantly a proprietary combination of synthetic growth factors.
This provides a consistent and controlled environment for cell growth and research. Noticeably, CD-IMDM is essential for applications that require reproducibility and regulatory compliance as well as for studying specific cellular processes, without extraneous variables. Furthermore, CD-IMDM is designed for custom formulations tailored for various cell types
Important Notes:
It is crucially important to have a gradual transition from media containing FBS to chemically defined cell culture media. Follow these steps:
- Starting Point: 10% FBS+ (control) (you need two controls, one to be left in only 10% FBS and continue monitoring for comparing with 0% FBS + PurMaTM Chemically Defined Medium. The second one is chemically defined medium + 10% FBS, which is going through the reduction of FBS process
- 48 hours later: 8% FBS in + PurMaTM Chemically Defined Medium
- 48 hours later: 6% FBS in + PurMaTM Chemically Defined Medium
- 48 hours later: 4% FBS + PurMaTM Chemically Defined Medium
- 48 hours later: 2% FBS + PurMaTM Chemically Defined Medium
- 48 hours later: 1% FBS + PurMaTM Chemically Defined Medium
- 24 hours later: 0% FBS and only PurMaTM Chemically Defined Medium
Importantly, for each change, spin down and fully discard the media and replace it with the next lower percentage of FBS.
Noticeably, you don’t have to start with a huge number of cells (~500,000 cells/ml). Additionally, we strongly recommend you start with more than one plate as some of the plates might get contaminated.
So, once the cells are kept in chemically defined Media, you should always keep them in the same chemically defined medium from the same vendor and the same formulation, even for freezing (see below).
Usually, after you get your cells adapted to PurMaTM Chemically Defined Medium you need to make several plates and freeze down several vials. The mentioned cells are adapted to chemically defined medium and when you thaw them in the first week, in addition to PurMaTM Chemically Defined Medium, you need to add 10% PurMaTM CD Booster (Cat# P3C10144).
Formulation
The CD-IMDM includes a base formulation including L-Alanine -L glutamine :Base Formulation of CD IMDM P3C310107
Additionally, CD-IMDM contains a proprietary synthetic cocktail of specific growth factors, hormones, and other nutrients which makes it independent of any serum and any trace of animal products.
References
- Growth of NS0 cells in protein-free, chemically defined medium.
- Complete replacement of serum by albumin, transferrin, and soybean lipid in cultures of lipopolysaccharide-reactive B lymphocytes. J Exp Med. 1978 Mar 1; 147(3): 923–933. doi: 10.1084/jem.147.3.923 PMCID: PMC2184195
- Effects of glutamine supply on growth and metabolism of mammalian cells in chemostat culture. Vriezen N, et al. Biotechnol Bioeng. 1997 May 5;54(3):272-86. doi: 10.1002/(SICI)1097-0290(19970505)54:3<272::AID-BIT8>3.0.CO;2-C. PMID: 18634093.
| Parameter | Specification |
|---|---|
| Appearance | Red, clear liquid |
| pH | 7.2 ± 0.1 |
| Osmolality | 299-344 mOsm/L |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Suitability | Suitable for mammalian cell culture |
| Additive | Sodium pyruvate |
| Indicator | Phenol red |
| Mycoplasma Detection | Negative |
| Sterility Tested | Sterile filtered using 0.1 µm filter, 3 times |
| Form | Liquid |
| Shipping Condition | Room temperature. Overnight |
Learn more: What Is IMDM?
Additional information
| Condition | CD-IMDM Standard Formulation, CD-IMDM w/o L-Alanyl-L-Glutamine, CD-IMDM w/o Phenol Red, CD-IMDM w/o Sodium Bicarbonate, CD-IMDM w/o Sodium Pyruvate, CD-IMDM w/o Glucose, CD-IMDM w/o HEPES, CD-IMDM w/o L-Alanyl-L-Glutamine, w/o Phenol Red, CD-IMDM w/o Phenol Red, w/o Sodium Bicarbonate, CD-IMDM w/o Sodium Bicarbonate, w/o Sodium Pyruvate, CD-IMDM w/o L-Alanyl-L-Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, CD-IMDM Low Glucose (1.5 g/L), CD-IMDM 15 mM HEPES (3.6 g/L), CD-IMDM 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.5 g/L), CD-IMDM w/o Sodium Pyruvate, w/o Phenol Red, CD-IMDM w/o Sodium Pyruvate, w/o HEPES, CD-IMDM w/o Phenol Red, with 15 mM HEPES (3.6 g/L), CD-IMDM w/o Sodium Pyruvate, with 15 mM HEPES (3.6 g/L), CD-IMDM w/o Phenol Red, Low Sodium Bicarbonate (1.5 g/L), CD-IMDM w/o Sodium Pyruvate, Low Sodium Bicarbonate (1.5 g/L) |
|---|---|
| Size | 1X 100 ml, 1X 1000 ml, 1X 500 ml |
Documents
Certificate of Analysis (COA): Available upon request for your specific order — email info@purmabiologics.com and we will send the current lot documentation.
Safety Data Sheet (SDS): Available upon request — email info@purmabiologics.com.
What our customers say about PurMa Biologics
Feedback from labs we supply, across our full catalogue.
We have been working with PurMa biologics for the past several years, we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum. We appreciate the high quality and reasonable price, and great customer service.
We purchase everything we need related to cell and tissue culture from PurMa, reagents like: DMEM, LL15, EMEM, FBS, trypsin, antibiotics mix100X and... Always great quality, fast processing and shipping. We are totally satisfied.
Very fast, efficient, and economic service. Great for new PIs like me.
it is reliable vendor!
We always buy our agarose from PurMa and have never been disappointed.
I recently had the pleasure of interacting with Dr. Seif Azadi from Purma Biologics, and I cannot thank him enough for his expertise and prompt assistance. His deep knowledge and dedication were instrumental in meeting our laboratory’s needs with remarkable efficiency. We ordered FBS, DMEM, and Trypsin from Purma Biologics, and I’m thrilled to report that all the products have performed exceptionally well. They consistently deliver reproducible results, significantly enhancing the reliability of our experiments. Thank you, Team Purma Biologics, for your good products and support!
Frequently asked questions
How should I store Chemically Defined Iscove’s Modified Dulbecco’s Medium (CD-IMDM)?
Store at 2 to 8 degrees C, protected from light, and do not freeze unless the label states otherwise. Freezing can precipitate salts and degrade light sensitive components such as riboflavin and folic acid. Always follow the storage line on the Certificate of Analysis for the specific lot.
What is the shelf life once opened?
Unopened and correctly stored, the typical shelf life is 12 months from manufacture. Once opened, use within about four weeks and always handle in a laminar flow hood, since the practical limit after opening is contamination risk rather than chemical stability.
Is it sterility and endotoxin tested?
Yes. Every lot is sterile filtered and tested for sterility and endotoxin, and a Certificate of Analysis is issued for each lot. The COA is available on request for any catalogue number.
Do I need to add L-glutamine?
It depends on the formulation you order. Standard formulations may contain free L-glutamine, which degrades in solution over time, while MAX formulations use a stable L-alanyl-L-glutamine dipeptide that does not require fresh supplementation. Check the option you have selected before adding more.
Can I order this in bulk or as a custom formulation?
Yes. Bulk volumes and custom formulations are made to order, including adjusted glucose, bicarbonate, buffering and cation content. Use the request bulk pricing button on this page and include the specification you need.
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