Chemically Defined Iscove’s Modified Dulbecco’s Medium (CD-IMDM)

Description

Background

PurMa^TM Chemically Defined Iscove’s Modified Dulbecco’s Medium (CD-IMDM) is a proprietary formulation used to grow cells in vitro without any undefined or animal-derived components. Moreover, CD-IMDM contains known quantities of pure chemicals, including amino acids, vitamins, salts, sugars, and more importantly a proprietary combination of synthetic growth factors.

This provides a consistent and controlled environment for cell growth and research. Noticeably, CD-IMDM is essential for applications that require reproducibility and regulatory compliance as well as for studying specific cellular processes, without extraneous variables. Furthermore, CD-IMDM is designed for custom formulations tailored for various cell types

Important Notes:

It is crucially important to have a gradual transition from media containing FBS to chemically defined cell culture media. Follow these steps:

• Starting Point: 10% FBS+ (control) (you need two controls, one to be left in only 10% FBS and continue monitoring for comparing with 0% FBS + PurMa^TM Chemically Defined Medium. The second one is chemically defined medium + 10% FBS, which is going through the reduction of FBS process
• 48 hours later: 8% FBS in + PurMa^TM Chemically Defined Medium
• 48 hours later: 6% FBS in + PurMa^TM Chemically Defined Medium
• 48 hours later: 4% FBS + PurMa^TM Chemically Defined Medium
• 48 hours later: 2% FBS + PurMa^TM Chemically Defined Medium
• 48 hours later: 1% FBS + PurMa^TM Chemically Defined Medium
• 24 hours later: 0% FBS and only PurMa^TM Chemically Defined Medium

Importantly, for each change, spin down and fully discard the media and replace it with the next lower percentage of FBS.

Noticeably, you don’t have to start with a huge number of cells (~500,000 cells/ml). Additionally, we strongly recommend you start with more than one plate as some of the plates might get contaminated.

So, once the cells are kept in chemically defined Media, you should always keep them in the same chemically defined medium from the same vendor and the same formulation, even for freezing (see below).

Usually, after you get your cells adapted to PurMa^TM Chemically Defined Medium you need to make several plates and freeze down several vials. The mentioned cells are adapted to chemically defined medium and when you thaw them in the first week, in addition to PurMa^TM Chemically Defined Medium, you need to add 10% PurMa^TM CD Booster (Cat# P3C10144).

Formulation

The CD-IMDM includes a base formulation including L-Alanine -L glutamine :Base Formulation of CD IMDM P3C310107

Additionally, CD-IMDM contains a proprietary synthetic cocktail of specific growth factors, hormones, and other nutrients which makes it independent of any serum and any trace of animal products.

References

1. Growth of NS0 cells in protein-free, chemically defined medium. Gorfien S, et al,Biotechnol Prog. 2000 Sep-Oct;16(5):682-7. doi: 10.1021/bp000109a. PMID: 11027156
2. Complete replacement of serum by albumin, transferrin, and soybean lipid in cultures of lipopolysaccharide-reactive B lymphocytes. J Exp Med. 1978 Mar 1; 147(3): 923–933. doi: 10.1084/jem.147.3.923 PMCID: PMC2184195
3. Effects of glutamine supply on growth and metabolism of mammalian cells in chemostat culture. Vriezen N, et al. Biotechnol Bioeng. 1997 May 5;54(3):272-86. doi: 10.1002/(SICI)1097-0290(19970505)54:3<272::AID-BIT8>3.0.CO;2-C. PMID: 18634093.

Learn more: What Is IMDM?