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- ✓Backed by decades of cell culture and protein biochemistry expertise
- ✓Bulk pricing, purchase orders, and custom formulations welcome
McCoy’s 5A Modified Media (M5AMM)
McCoy’s 5A Modified Media (M5AMM) is widely used cor tumor cells. Additionally, modified version of this media contains several folds of amino acids and vitamins compared to the original formulation.
Description
Background
McCoy’s 5A Modified Medium (M5AMM) was originally used for Walker Carcinosarcoma 256 Cells. It was subsequently modified to create a new medium known as McCoy’s 5A. Additionally, McCoy’s 5A Modified Media (M5AMM) contains several folds of amino acids and vitamins compared to the original formulation. Our McCoy’s 5A covers the main needs for your cell lines, delivers consistency, excellent quality, and the full control you need for mammalian cell culture.
MCCOY’S 5A Contains:
- High glucose (3g/L)
- Non-Essential Amino Acids
- Phenol Red
- L-glutamine
Why PurMa Biologics? Why we stand behind our cell culture media:
Our products are the outcome of 30 years of experience in cutting edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. We utilize 99% pure amino acids. This will significantly increase the consistency among your experiments, and the result of your lab. Considering the highest quality, our prices are extremely reasonable.
Application
McCoy’s 5A Modified Media (M5AMM) is widely used for commercial cell lines such as CHO, HEK 293, HeLa, as well as Jurkat cells. Moreover, PurMaTM McCoy’s Medium contains no proteins, lipids, or growth factors. Therefore, McCoy’s 5A Modified Media (M5AMM) requires supplementation, commonly with 10% Fetal Bovine Serum (FBS).
Formulation
complete formulation is available in formulation tab as well as here:McCoy’s 5A Formulation
References
- Investigations on in vitro survival and virulence of T. pallidum under aerobiosis. Rathlev T. Br J Vener Dis. 1975 Oct;51(5):296-300. doi: 10.1136/sti.51.5.296. PMID: 172190
- Mycoplasmal infection of insect cell cultures. Steiner et al.In Vitro. 1983 Sep;19(9):672-82. doi: 10.1007/BF02628958.PMID: 6413389.
- In vitro culture of postimplantation hamster embryos. Ebron-et al. Reprod Toxicol. 1988;2(1):31-6. doi: 10.1016/s0890-6238(88)80006-6.PMID: 2980399
| Parameter | Specification |
|---|---|
| Appearance | Red, clear liquid |
| pH | 7.2 ± 0.1 |
| Osmolality | 275-360 mOsm/L |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Suitability | Suitable for mammalian cell culture |
| Additive | Sodium pyruvate |
| Indicator | Phenol red |
| Mycoplasma Detection | Negative |
| Sterility Tested | Sterile filtered using 0.22 µm filter |
| Form | Liquid |
| Shipping Condition | Room temperature |
Learn more: What Is McCoy’s 5A?
Additional information
| Condition | M5AMM- Standard Formulation, M5AMM Without Glutamine, M5AMM -Without Phenol Red, M5AMM -Without Sodium Bicarbonate, M5AMM – Without Glucose, M5AMM – Without Peptone, M5AMM – w/o Glutamine, w/o Phenol Red, M5AMM – Without Phenol Red, Without Sodium Bicarbonate, M5AMM – w/o Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, M5AMM – Low Glucose (1.5 g/L), M5AMM – Low Sodium Bicarbonate (0.35 g/L), M5AMM – Low Sodium Bicarbonate (0.85 g/L), M5AMM -15 mM HEPES (3.6 g/L), M5AMM – 25 mM HEPES (5.9 g/L |
|---|---|
| Format | Liquid, Powder |
| Size | 10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L |
Documents
Certificate of Analysis (COA): Available upon request for your specific order — email info@purmabiologics.com and we will send the current lot documentation.
Safety Data Sheet (SDS): Available upon request — email info@purmabiologics.com.
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