PurMaTM McCoy’s 5A Medium was originally used to perform analysis on Walker Carcinosarcoma 256 Cells, and subsequently modified to create a new medium known as McCoy’s 5A Medium. PurMaTM McCoy’s 5A Medium contains several folds of amino acids and vitamins compared to the original formulation.  There are several versions of McCoy’s 5A available in market. McCoy’s 5A produced by PurMa Biologics covers the main needs for most of the cell’s lines, delivers consistency, excellent quality, and the and the full control that needed for mammalian cell culture. PurMaTM McCoy’s 5A contains no proteins, lipids, or growth factors. Therefore, it requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). This Advanced MCCOY’S 5A contains:

  • High glucose (3g/L)
  • Non-Essential Amino Acids
  • Phenol Red
  • L-glutamine

Why PurMa Biologics? Why we stand behind our cell culture media:

  1. Our products are the outcome of 30 years of experience in cutting edge cell and tissue culture science.
  2. We routinely manufacture over 1500 media which is the most comprehensive collection of cell and tissue culture media.
  3. We utilize %99 pure amino acids. This will significantly increase the consistency among your experiments, and the result of your lab compared to other laboratories working on the same issues.
  4. Considering the highest quality, our prices are extremely reasonable.

Application of PurMaTM McCoy’s

This media has elements that prevent clumping the cells which is a necessity for performing analysis on these cells. Ham’s F-12K has been formulated for use in a 5% carbon dioxide atmosphere and is not recommended for higher and lower percentages of CO2. It utilizes a bicarbonate buffering system. Scientists in PurMa Biologics have successfully evaluated PurMaTM McCoy’s medium for the culture of vast varieties of primary Skin cells, insect cells,  as well as bovine preantral follicles, post implantation hamster embryos. McCoy’s 5A Medium is widely used for commercial cell line as CHO, HEK 293, HeLa and  Jurkat cells. PurMaTM McCoy’s Medium contains no proteins, lipids, or growth factors. Therefore, Ham’s F-12 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS).

Formulation: for complete formulation click here: Regular McCoy Formulation

  1. The survival of human skin stored by refrigeration at 4 degrees C in McCoys 5A medium: does oxygenation of the medium improve storage time? DeBono R, Rao GS, Berry RB.Plast Reconstr Surg. 1998 Jul;102(1):78-83. doi: 10.1097/00006534-199807000-00012.PMID: 9655410
  2. Mycoplasmal infection of insect cell cultures. Steiner T, McGarrity G.In Vitro. 1983 Sep;19(9):672-82. doi: 10.1007/BF02628958.PMID: 6413389
  3. Fresh and vitrified bovine preantral follicles have different nutritional requirements during in vitro culture. Castro SV, Carvalho AA, Silva CM, Santos FW, Campello CC, Figueiredo JR, Rodrigues AP.Cell Tissue Bank. 2014 Dec;15(4):591-601. doi: 10.1007/s10561-014-9432-2. Epub 2014 Mar 8.PMID: 24610241
  4. In vitro culture of postimplantation hamster embryos. Ebron-McCoy MT, Beyer PE, Oglesby LA, Kavlock RJ.Reprod Toxicol. 1988;2(1):31-6. doi: 10.1016/s0890-6238(88)80006-6.PMID: 2980399
  5. The turkey myogenic satellite cell: optimization of in vitro proliferation and differentiation. McFarland DC, Doumit ME, Minshall RD.Tissue Cell. 1988;20(6):899-908. doi: 10.1016/0040-8166(88)90031-6.PMID: 3245037
  6. Investigations on in vitro survival and virulence of T. pallidum under aerobiosis. Rathlev T. Br J Vener Dis. 1975 Oct;51(5):296-300. doi: 10.1136/sti.51.5.296. PMID: 172190
  7. Altered polyamine metabolism in Chinese hamster cells growing in a defined medium. Sertich GJ, Glass JR, Fuller DJ, Gerner EW. J Cell Physiol. 1986 Apr;127(1):114-20. doi: 10.1002/jcp.1041270115. PMID: 3958058
Parameter Specification
Appearance Red, clear liquid
pH  7.2 ± 0.1
Osmolality  275-360 mOsm/L
Endotoxin  NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive  Sodium pyruvate
Indicator  Phenol red
Mycoplasma Detection Negative
Sterility Tested  Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature