Advanced Iscove’s Modified Dulbecco’s Medium (A-IMDM)

Advanced Iscove’s Modified Dulbecco’s Medium (A-IMDM) contains L-Alanyl-L-Glutamine as well as ingredients to allow for serum reduction.  Additionally, A-IMDM needs 2-5% FBS and provides more consistency.

Description

Advanced Iscove’s Modified Dulbecco’s Medium (A-IMDM)

Background

PurMaTM Advanced Iscove’s Modified Dulbecco’s Medium (A-IMDM) contains components that allow for a 60–90% reduction in FBS supplementation. More importantly, using synthetic components such as recombinant insulin, albumin, and transferrin increase consistency. This results in less variation amongst lot-to-lot serum changes. In A-IMDM, L-Alanyl-L-Glutamine is used as the source of glutamine. Additionally, reducing FBS requirements lowers the cost of cell culture experiments.

Contents

Advanced IMDM Contains:

  • includes all twenty essential and non-essential amino acids with higher concentrations than regular and MAX formulations.
  • A-IMDM incorporates elements that significantly reduce the need for a serum for cell culture.

Crucial Ingredients:

  • PurMaTM Human Transferrin (Holo): prevents Improper iron storage and delivery in cell culture systems which is a major contributor to oxidative stress and protein damage.
  • PurMaTM Insulin Recombinant
  • Trace of other elements which mimic the elements in fetal bovine serum.

Consequently, Advanced Iscove’s Modified Dulbecco’s Medium (A-IMDM) is a significant step toward a chemically defined form of this media. Unquestionably, Chemically Defined Advanced-IMDM (CD-A-IMDM) is the outcome of decades of cutting-edge research and will soon be offered globally to laboratories that are looking for consistency and repeatability in their data.

Formulation

Complete formulation of Advanced IMDM: Advanced IMDM Formulation

References

 

Parameter Specification
Appearance Red, clear liquid
pH  7.2 ± 0.1
Osmolality  275-360 mOsm/L
Endotoxin  NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive  Sodium pyruvate
Indicator  Phenol red
Mycoplasma Detection Negative
Sterility Tested  Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

 

Additional information

Condition

A-IMDM Standard Formulation, A-IMDM w/o L-Alanyl-L-Glutamine, A-IMDM w/o Phenol Red, A-IMDM w/o Sodium Bicarbonate, A-IMDM w/o Sodium Pyruvate, A-IMDM w/o Glucose, A-IMDM w/o HEPES, A-IMDM w/o L-Alanyl-L-Glutamine, w/o Phenol Red, A-IMDM w/o Phenol Red, w/o Sodium Bicarbonate, A-IMDM w/o Sodium Bicarbonate, w/o Sodium Pyruvate, A-IMDM w/o L-Alanyl-L-Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, A-IMDM w/o Phenol Red, w/o Sodium Bicarbonate, w/o Sodium Pyruvate, A-IMDM w/o L-Alanyl-L-Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, w/o Sodium Pyruvate, A-IMDM Low Glucose (1.5 g/L), A-IMDM Low Sodium Bicarbonate (1.5 g/L), A-IMDM 15 mM HEPES (3.6 g/L), A-IMDM 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.5 g/L), A-IMDM w/o Sodium Pyruvate, w/o Phenol Red, A-IMDM w/o Sodium Pyruvate, w/o HEPES, A-IMDM w/o phenol Red, w/o Sodium Pyruvate, w/o HEPES, A-IMDM w/o Phenol Red, 15 mM HEPES (3.6 g/L), A-IMDM w/o Sodium Pyruvate, with 15 mM HEPES (3.6 g/L), A-IMDM w/o Phenol Red, Low Sodium Bicarbonate (1.5 g/L), A-IMDM w/o Sodium Pyruvate, Low Sodium Bicarbonate (1.5 g/L), A-IMDM w/o Phenol Red, w/o Sodium Pyruvate, Low Sodium Bicarbonate (1.5 g/L), A-IMDM w/o Sodium Pyruvate, w/o Phenol Red, with 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.5 g/L), A-IMDM w/o Phenol Red, w/o Sodium Pyruvate, with 15 mM HEPES (3.6 g/L), A-IMDM w/o Phenol Red, with 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.5 g/L), A-IMDM w/o Sodium Pyruvate, 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.5g/L)

Format

Liquid, Powder

Size

10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed

Quality Control

[vc-quality-control-tab]

Formulation

Advanced IMDM

SDS

SDS

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