Advanced Iscove’s Modified Dulbecco’s Medium (A-IMDM)
Advanced Iscove’s Modified Dulbecco’s Medium (A-IMDM) contains L-Alanyl-L-Glutamine as well as ingredients to allow for serum reduction. Additionally, A-IMDM needs 2-5% FBS and provides more consistency.
Description
Advanced Iscove’s Modified Dulbecco’s Medium (A-IMDM)
Background
PurMaTM Advanced Iscove’s Modified Dulbecco’s Medium (A-IMDM) contains components that allow for a 60–90% reduction in FBS supplementation. More importantly, using synthetic components such as recombinant insulin, albumin, and transferrin increase consistency. This results in less variation amongst lot-to-lot serum changes. In A-IMDM, L-Alanyl-L-Glutamine is used as the source of glutamine. Additionally, reducing FBS requirements lowers the cost of cell culture experiments.
Contents
Advanced IMDM Contains:
- includes all twenty essential and non-essential amino acids with higher concentrations than regular and MAX formulations.
- A-IMDM incorporates elements that significantly reduce the need for a serum for cell culture.
Crucial Ingredients:
- PurMaTM Human Transferrin (Holo): prevents Improper iron storage and delivery in cell culture systems which is a major contributor to oxidative stress and protein damage.
- PurMaTM Insulin Recombinant
- Trace of other elements which mimic the elements in fetal bovine serum.
Consequently, Advanced Iscove’s Modified Dulbecco’s Medium (A-IMDM) is a significant step toward a chemically defined form of this media. Unquestionably, Chemically Defined Advanced-IMDM (CD-A-IMDM) is the outcome of decades of cutting-edge research and will soon be offered globally to laboratories that are looking for consistency and repeatability in their data.
Formulation
Complete formulation of Advanced IMDM: Advanced IMDM Formulation
References
- Plaque production by the polyoma virus. DULBECCO R, FREEMAN G. 1959 Jul;8(3):396-7. doi: 10.1016/0042-6822(59)90043-1. PMID: 13669362.
- Complete replacement of serum by albumin, transferrin, and soybean lipid in cultures of lipopolysaccharide-reactive B lymphocytes. J Exp Med. 1978 Mar 1; 147(3): 923–933. doi: 10.1084/jem.147.3.923 PMCID: PMC2184195
- Effects of glutamine supply on growth and metabolism of mammalian cells in chemostat culture. Vriezen N, et al. Biotechnol Bioeng. 1997 May 5;54(3):272-86. doi: 10.1002/(SICI)1097-0290(19970505)54:3<272::AID-BIT8>3.0.CO;2-C. PMID: 18634093.
| Parameter | Specification |
|---|---|
| Appearance | Red, clear liquid |
| pH | 7.2 ± 0.1 |
| Osmolality | 275-360 mOsm/L |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Suitability | Suitable for mammalian cell culture |
| Additive | Sodium pyruvate |
| Indicator | Phenol red |
| Mycoplasma Detection | Negative |
| Sterility Tested | Sterile filtered using 0.22 µm filter |
| Form | Liquid |
| Shipping Condition | Room temperature |
Additional information
| Condition | A-IMDM Standard Formulation, A-IMDM w/o L-Alanyl-L-Glutamine, A-IMDM w/o Phenol Red, A-IMDM w/o Sodium Bicarbonate, A-IMDM w/o Sodium Pyruvate, A-IMDM w/o Glucose, A-IMDM w/o HEPES, A-IMDM w/o L-Alanyl-L-Glutamine, w/o Phenol Red, A-IMDM w/o Phenol Red, w/o Sodium Bicarbonate, A-IMDM w/o Sodium Bicarbonate, w/o Sodium Pyruvate, A-IMDM w/o L-Alanyl-L-Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, A-IMDM w/o Phenol Red, w/o Sodium Bicarbonate, w/o Sodium Pyruvate, A-IMDM w/o L-Alanyl-L-Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, w/o Sodium Pyruvate, A-IMDM Low Glucose (1.5 g/L), A-IMDM Low Sodium Bicarbonate (1.5 g/L), A-IMDM 15 mM HEPES (3.6 g/L), A-IMDM 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.5 g/L), A-IMDM w/o Sodium Pyruvate, w/o Phenol Red, A-IMDM w/o Sodium Pyruvate, w/o HEPES, A-IMDM w/o phenol Red, w/o Sodium Pyruvate, w/o HEPES, A-IMDM w/o Phenol Red, 15 mM HEPES (3.6 g/L), A-IMDM w/o Sodium Pyruvate, with 15 mM HEPES (3.6 g/L), A-IMDM w/o Phenol Red, Low Sodium Bicarbonate (1.5 g/L), A-IMDM w/o Sodium Pyruvate, Low Sodium Bicarbonate (1.5 g/L), A-IMDM w/o Phenol Red, w/o Sodium Pyruvate, Low Sodium Bicarbonate (1.5 g/L), A-IMDM w/o Sodium Pyruvate, w/o Phenol Red, with 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.5 g/L), A-IMDM w/o Phenol Red, w/o Sodium Pyruvate, with 15 mM HEPES (3.6 g/L), A-IMDM w/o Phenol Red, with 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.5 g/L), A-IMDM w/o Sodium Pyruvate, 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.5g/L) |
|---|---|
| Format | Liquid, Powder |
| Size | 1 X 10L ( Powder), 1 X 50 L (Powder), 10 X 1EZ Individually Packed (Powder), 50 X 1EZ Individually Packed (Powder), 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml |
Quality Control
[vc-quality-control-tab]Formulation
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