- ✓Proudly made in the USA, manufactured in house
- ✓Certificate of Analysis available for every lot
- ✓Backed by decades of cell culture and protein biochemistry expertise
- ✓Bulk pricing, purchase orders, and custom formulations welcome
PurMa™ Dulbecco’s Modified Eagle’s Medium/F-12 (DMEM F-12)
A high-performance DMEM/F-12 formulation engineered for consistent mammalian cell culture.
- Enriched with 2.5 mM L-glutamine, 0.5 mM sodium pyruvate, 30 mM NaHCO3, high glucose, non-essential amino acids, and phenol red
- Ideal for MDCK, glial cells, fibroblasts, and human endothelial cultures
Description
PurMa™ Dulbecco’s Modified Eagle’s Medium/F-12 (DMEM F-12)
Background
PurMa™ Dulbecco’s Modified Eagle’s Medium/F-12 (DMEM F-12) is an enriched DMEM/F‑12 formulation developed for reliable, reproducible mammalian cell culture and optimized for cell types that benefit from additional nutrients and buffering. This advanced DMEM F‑12 supports MDCK, glial cells, fibroblasts, human endothelial cells and rat fibroblasts, providing consistent performance in research and production workflows.
Special Features
- L‑Glutamine: 2.5 mM (stabilized where applicable)
- Sodium pyruvate: 0.5 mM
- Sodium bicarbonate (NaHCO3): 30 mM (2.438 g/L) for robust CO2 buffering
- High glucose level for energy‑intensive cell types
- Non‑essential amino acids to support protein synthesis and growth
- Phenol red pH indicator for culture monitoring
Why choose PurMa Advanced DMEM F‑12 (DMEM F‑12 formulation advantages)
- Consistency: Manufactured under stringent QC for lot‑to‑lot uniformity.
- Moreover, enriched composition of advanced DMEM F‑12: Compared with standard DMEM, DMEM F‑12 provides additional pyruvate, amino acids and optimized buffering—see “DMEM f12 vs DMEM” for differences in nutrient profile and application.
- Versatility: Well‑suited for a broad range of adherent and suspension mammalian cell lines that demand enriched media.
- Ready for supplementation: Compatible with serum, defined supplements, growth factors, and antibiotics per your protocol.
Applications
- Advanced Dulbecco’s Modified Eagle’s Medium/F-12 (DMEM F-12) is suitable for MDCK cell culture
- Glial and neural cell maintenance
- Primary and immortalized fibroblast culture
- Human endothelial cell expansion
- Routine cell maintenance, proliferation assays, and transfection workflows
Handling & Storage
- Store Advanced Dulbecco’s Modified Eagle’s Medium/F-12 (DMEM F-12) unopened at 2–8 °C protected from light.
- Warm to 37 °C before use and handle aseptically.
- Use prepared Advanced DMEM F‑12 according to your lab SOPs; supplement with serum or additives as required.
Quality & Support Dulbecco’s Modified Eagle’s Medium/F-12 (DMEM F-12) is produced in a controlled manufacturing environment with full documentation and Certificate of Analysis available for each lot. Contact technical support for media optimization and supplementation guidance.
Formulation
For complete formulation click here which is identical to thermofisher and gibco advanced dmemf12: DMEM F-12 Formulation
References:
- Clonal growth of mammalian cells in a chemically defined, synthetic medium . DULBECCO et al. 1959 Jul;8(3):396-7. doi: 10.1016 /0042-68 22(59)90043-1. PMID: 13669362.
- Plaque production by the polyoma virus. DULBECCO et al. 1959 Jul;8(3):396-7. doi: 10.1016/0042-6822(59)90043-1. PMID: 1366936.
| Parameter | Specification |
|---|---|
| Appearance | Red, clear liquid |
| pH | 7.2 ± 0.1 |
| Osmolality | 275-360 mOsm/L |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Suitability | Suitable for mammalian cell culture |
| Additive | Sodium pyruvate |
| Indicator | Phenol red |
| Mycoplasma Detection | Negative |
| Sterility Tested | Sterile filtered using 0.22 µm filter |
| Form | Liquid |
| Shipping Condition | Room temperature |
Learn more: What Is Ham’s F-12K?
Additional information
| Condition | DMEM F-12 Standard Formulation, DMEM F-12 Standard Formulation, ATCC Formulation, DMEM F-12 w/o Glutamine, DMEM F-12 w/o Phenol Red, DMEM F-12 w/o Sodium Bicarbonate, DMEM F-12 w/o Sodium Pyruvate, DMEM F-12 w/o Glucose, DMEM F-12 w/o Lipoic Acid, DMEM F-12 w/o Glutamine, w/o Phenol Red, DMEM F-12 w/o Phenol Red, w/o Sodium Bicarbonate, DMEM F-12 w/o Sodium Bicarbonate, w/o Sodium Pyruvate, DMEM F-12 w/o Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, DMEM F-12 w/o Phenol Red, w/o Sodium Bicarbonate, w/o Sodium Pyruvate, DMEM F-12 Low Glucose (1.5 g/L), DMEM F-12 Low Sodium Bicarbonate (1.2 g/L), DMEM F-12 15 mM HEPES (3.6 g/L), DMEM F-12 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.2 g/L), DMEM F-12 w/o Sodium Pyruvate, w/o phenol Red, DMEM F-12 w/o Phenol Red, with 15 mM HEPES (3.6 g/L), DMEM F-12 w/o Phenol Red, Low Sodium Bicarbonate (1.2 g/L), DMEM F-12 w/o Sodium Pyruvate, Low Sodium Bicarbonate (1.2 g/L), DMEM F-12 High Glucose (4.5 g/L) |
|---|---|
| Format | Liquid, Powder |
| Size | 10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L |
Documents
Certificate of Analysis (COA): Available upon request for your specific order — email info@purmabiologics.com and we will send the current lot documentation.
Safety Data Sheet (SDS): Available upon request — email info@purmabiologics.com.
What our customers say about PurMa Biologics
Feedback from labs we supply, across our full catalogue.
We purchase everything we need related to cell and tissue culture from PurMa, reagents like: DMEM, LL15, EMEM, FBS, trypsin, antibiotics mix100X and... Always great quality, fast processing and shipping. We are totally satisfied.
Very fast, efficient, and economic service. Great for new PIs like me.
it is reliable vendor!
We always buy our agarose from PurMa and have never been disappointed.
I recently had the pleasure of interacting with Dr. Seif Azadi from Purma Biologics, and I cannot thank him enough for his expertise and prompt assistance. His deep knowledge and dedication were instrumental in meeting our laboratory’s needs with remarkable efficiency. We ordered FBS, DMEM, and Trypsin from Purma Biologics, and I’m thrilled to report that all the products have performed exceptionally well. They consistently deliver reproducible results, significantly enhancing the reliability of our experiments. Thank you, Team Purma Biologics, for your good products and support!
It’s been a pleasure working with PURMA Biologics. Their service is efficient, delivery is fast, and the quality of their products has met our expectations consistently. Very smooth collaboration overall.
Frequently asked questions
How should I store PurMa™ Dulbecco’s Modified Eagle’s Medium/F-12 (DMEM F-12)?
Store at 2 to 8 degrees C, protected from light, and do not freeze unless the label states otherwise. Freezing can precipitate salts and degrade light sensitive components such as riboflavin and folic acid. Always follow the storage line on the Certificate of Analysis for the specific lot.
What is the shelf life once opened?
Unopened and correctly stored, the typical shelf life is 12 months from manufacture. Once opened, use within about four weeks and always handle in a laminar flow hood, since the practical limit after opening is contamination risk rather than chemical stability.
Is it sterility and endotoxin tested?
Yes. Every lot is sterile filtered and tested for sterility and endotoxin, and a Certificate of Analysis is issued for each lot. The COA is available on request for any catalogue number.
Do I need to add L-glutamine?
It depends on the formulation you order. Standard formulations may contain free L-glutamine, which degrades in solution over time, while MAX formulations use a stable L-alanyl-L-glutamine dipeptide that does not require fresh supplementation. Check the option you have selected before adding more.
Can I order this in bulk or as a custom formulation?
Yes. Bulk volumes and custom formulations are made to order, including adjusted glucose, bicarbonate, buffering and cation content. Use the request bulk pricing button on this page and include the specification you need.
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