McCoy’s 5A Modified Media MAX (M5AMM-MAX)

McCoy’s 5A Modified Media MAX (M5AMM-MAX) contains L-Alanyl-L-Glutamine, therefore supplying a longer shelf life.   McCoy’s 5A MAX significantly improves cell viability and growth. We manufacture several types of McCoy’s 5A MAX based on your requirements.

Description

McCoy’s 5A Modified Media-MAX (M5AMM-MAX)

Background

McCoy’s 5A Modified Media MAX (M5AMM-MAX) is a commonly used media for Mammalian cell culture.

Advantages

The advantages of McCoy’s 5A MAX over McCoy’s 5A  are:

  • Higher PH Capacity
  • Moreover, it contains 4 mM L-Alanyl-L-Glutamine (0.869 g/L) instead of L-Glutamine and therefore:
  • Possess longer shelf life.
  • More importantly, minimizes toxic ammonia build-up.
  • Significantly improves cell viability and growth.
  • Remains stable across a wide range of temperatures.
  • Improves the culture of primary cells.

Mechanism

Although L-glutamine is a vital amino acid, it degrades, resulting in the generation of toxic ammonia and pyrrolidine carboxylic acid. One way to minimize L-glutamine degradation in media is to gradually add these amino acids to your cell culture. However, keeping track of time to keep the amount of L-glutamine at the same level is tedious, if not impossible.

Alternatively, L-alanyl-L-glutamine can be used because it is much more stable in aqueous solutions. More importantly, it does not easily degrade and gradually releases aminopeptidases. As a result, L-glutamine and L-alanine are then used by the cells for protein production in the TCA cycle.

In addition to our Max formulation products, we manufacture PurMaTM GluaSup (Cat# P3S210 559) is a combination of L-alanyl-L-glutamine which prevents degradation of L-glutamine.

Contents

McCoy’s 5A Modified Media MAX (M5AMM-MAX) contains:

  • 5 mM sodium pyruvate
  • High glucose (4.5g/L)
  • Non-Essential Amino Acids
  • 3.7 g/L Sodium Bicarbonate; PurMa Biologics Manufactures several types of McCoy’s 5A  based on the amount of Sodium Bicarbonate and HEPES (PH capacity)
  • Phenol Red

McCoy’s 5A MAX contains no proteins, lipids, or growth factors. Therefore, it requires supplementation, commonly with 10% Fetal bovine Serum (FBS).

Formulation

The complete formulation can be found here: McCoy’s 5A MAX Formulation

References

  1. Mycoplasmal infection of insect cell cultures. Steiner T, McGarrity G. In Vitro. 1983 Sep;19(9):672-82. doi: 10.1007/BF02628958.PMID: 6413389
  2. Investigations on in vitro survival and virulence of T. pallidum under aerobiosis. Rathlev T. Br J Vener Dis. 1975 Oct;51(5):296-300. doi: 10.1136/sti.51.5.296. PMID: 172190
  3. Altered polyamine metabolism in Chinese hamster cells growing in a defined medium. Sertich GJ, et al. J Cell Physiol. 1986 Apr;127(1):114-20. doi: 10.1002/jcp.1041270115.PMID: 3958058
Parameter Specification
Appearance Red, clear liquid
pH  7.2 ± 0.1
Osmolality  275-360 mOsm/L
Endotoxin  NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive  Sodium pyruvate
Indicator  Phenol red
Mycoplasma Detection Negative
Sterility Tested  Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

 

Additional information

Condition

M5AMM-MAX- Standard Formulation, M5AMM-MAX -Without Phenol Red, M5AMM-MAX -Without Sodium Bicarbonate, M5AMM-MAX – Without Glucose, M5AMM-MAX – Without Peptone, M5AMM-MAX – Without Phenol Red, Without Sodium Bicarbonate, M5AMM-MAX – Low Glucose (1.5 g/L), M5AMM-MAX – Low Sodium Bicarbonate (0.35 g/L), M5AMM-MAX – Low Sodium Bicarbonate (0.85 g/L), M5AMM-MAX -15 mM HEPES (3.6 g/L), M5AMM-MAX – 25 mM HEPES (5.9 g/L), M5AMM-MAX – 15 mM HEPES (3.6 g/L) , Low Sodium Bicarbonate (0.35 g/L), M5AMM-MAX – 15 mM HEPES (3.6 g/L) , Low Sodium Bicarbonate (0.85g/L), M5AMM-MAX – 25 mM HEPES (3.6 g/L) , Low Sodium Bicarbonate (0.35 g/L), M5AMM-MAX – 25 mM HEPES (5.9 g/L) , Low Sodium Bicarbonate (0.85 g/L)

Format

Liquid, Powder

Size

10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed

Quality Control

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Formulation

McCoy’s 5A MAX Formulation

SDS

SDS

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