McCoy’s 5A Modified Media MAX (M5AMM-MAX) contains L-Alanyl-L-Glutamine, therefore supplying a longer shelf life. McCoy’s 5A MAX significantly improves cell viability and growth. We manufacture several types of McCoy’s 5A MAX based on your requirements.
McCoy’s 5A Modified Media MAX (M5AMM-MAX)
$34.10 – $705.50
Description
McCoy’s 5A Modified Media-MAX (M5AMM-MAX)
Background
McCoy’s 5A Modified Media MAX (M5AMM-MAX) is a commonly used media for Mammalian cell culture.
Advantages
The advantages of McCoy’s 5A MAX over McCoy’s 5A are:
- Higher PH Capacity
- Moreover, it contains 4 mM L-Alanyl-L-Glutamine (0.869 g/L) instead of L-Glutamine and therefore:
- Possess longer shelf life.
- More importantly, minimizes toxic ammonia build-up.
- Significantly improves cell viability and growth.
- Remains stable across a wide range of temperatures.
- Improves the culture of primary cells.
Mechanism
Although L-glutamine is a vital amino acid, it degrades, resulting in the generation of toxic ammonia and pyrrolidine carboxylic acid. One way to minimize L-glutamine degradation in media is to gradually add these amino acids to your cell culture. However, keeping track of time to keep the amount of L-glutamine at the same level is tedious, if not impossible.
Alternatively, L-alanyl-L-glutamine can be used because it is much more stable in aqueous solutions. More importantly, it does not easily degrade and gradually releases aminopeptidases. As a result, L-glutamine and L-alanine are then used by the cells for protein production in the TCA cycle.
In addition to our Max formulation products, we manufacture PurMaTM GluaSup (Cat# P3S210 559) is a combination of L-alanyl-L-glutamine which prevents degradation of L-glutamine.
Contents
McCoy’s 5A Modified Media MAX (M5AMM-MAX) contains:
- 5 mM sodium pyruvate
- High glucose (4.5g/L)
- Non-Essential Amino Acids
- 3.7 g/L Sodium Bicarbonate; PurMa Biologics Manufactures several types of McCoy’s 5A based on the amount of Sodium Bicarbonate and HEPES (PH capacity)
- Phenol Red
McCoy’s 5A MAX contains no proteins, lipids, or growth factors. Therefore, it requires supplementation, commonly with 10% Fetal bovine Serum (FBS).
Formulation
The complete formulation can be found here: McCoy’s 5A MAX Formulation
References
- Mycoplasmal infection of insect cell cultures. Steiner T, McGarrity G. In Vitro. 1983 Sep;19(9):672-82. doi: 10.1007/BF02628958.PMID: 6413389
- Investigations on in vitro survival and virulence of T. pallidum under aerobiosis. Rathlev T. Br J Vener Dis. 1975 Oct;51(5):296-300. doi: 10.1136/sti.51.5.296. PMID: 172190
- Altered polyamine metabolism in Chinese hamster cells growing in a defined medium. Sertich GJ, et al. J Cell Physiol. 1986 Apr;127(1):114-20. doi: 10.1002/jcp.1041270115.PMID: 3958058
Parameter | Specification |
---|---|
Appearance | Red, clear liquid |
pH | 7.2 ± 0.1 |
Osmolality | 275-360 mOsm/L |
Endotoxin | NMT< 2EU/mL |
Mycoplasma | Negative |
Suitability | Suitable for mammalian cell culture |
Additive | Sodium pyruvate |
Indicator | Phenol red |
Mycoplasma Detection | Negative |
Sterility Tested | Sterile filtered using 0.22 µm filter |
Form | Liquid |
Shipping Condition | Room temperature |
Formulation
Quality Control
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filter_opacity_hover="100" filter_blur_hover="0" transform_type="regular" transform_hover_element="self" transform_scale_x="1" transform_scale_y="1" transform_translate_x="0" transform_translate_y="0" transform_rotate="0" transform_skew_x="0" transform_skew_y="0" transform_scale_x_hover="1" transform_scale_y_hover="1" transform_translate_x_hover="0" transform_translate_y_hover="0" transform_rotate_hover="0" transform_skew_x_hover="0" transform_skew_y_hover="0" transform_origin="" transition_duration="300" transition_easing="ease" transition_custom_easing="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset="" last="true" border_position="all" first="true"][fusion_text columns="" column_min_width="" column_spacing="" rule_style="" rule_size="" rule_color="" hue="" saturation="" lightness="" alpha="" content_alignment_medium="" content_alignment_small="" content_alignment="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" sticky_display="normal,sticky" class="" id="" margin_top="" margin_right="" margin_bottom="" margin_left="" fusion_font_family_text_font="" fusion_font_variant_text_font="" font_size="" line_height="" letter_spacing="" text_transform="" text_color="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset=""]Minimum Essential Media (MEM) in Earle’s Buffer
Background
Minimum Essential Media (MEM) in Earle’s Buffer is a modification of Basal Medium Eagle (BME) which was originally developed by Harry Eagle. It also includes higher concentrations of amino acids for cultured mammalian cells. Moreover, MEM is available with Earle’s salts for use in a CO2 incubator, or with Hanks' salts for use without CO2.PurMa™ MEM media includes Earle's Buffer
As the composition of Earle's buffer, it contains:- Calcium chloride
- Magnesium sulfate
- Potassium chloride
- Sodium bicarbonate
- Sodium chloride
- Sodium Phosphate, monobasic
PurMa Biologics manufactures 118 types of MEM, it also includes:
- Low level of glucose (1.00 g/ liter)
- Sodium bicarbonate (2.2 g/L)
- 2 mM L-glutamine (292 mg/L)’
Why PurMa Biologics? Why we stand behind our cell culture media:
Our products are the outcome of 30 years of experience in cutting edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. Moreover, We utilize %99 pure amino acids. This will significantly increase the consistency among your experiments, and the result of your lab. Considering the highest quality, our prices certainly are extremely reasonable.Application:
MEM has been used for cell lines such as HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts as well as primary rat astrocytes. Additionally, MEM in Earle's buffer uses a sodium bicarbonate buffer system (2.2 g/L) that requires a 5–10% CO2 environment to maintain physiological pH.Formulation
For detailed formulation visit "the formulation Tab" as well as by clicking here: MEM Formulation in Earl's BufferReferences
- Culture of granulosa cells in collagen gels: the influence of cell shape on steroidogenesis. Carnegie et al. Biol Reprod. 1988 May;38(4):881-90. doi: 10.1095/biolreprod38.4.881. PMID: 3401543
- Myelin formation in rat dorsal root ganglion cultured in a serum-free medium. Ninomiya et al. J Anat. 1987 Jun;152:209-13. PMID: 365437
Parameter Specification Appearance Red, clear liquid pH 7.2 ± 0.1 Osmolality 275-360 mOsm/L Endotoxin NMT< 2EU/mL Mycoplasma Negative Suitability Suitable for mammalian cell culture Additive N/A Indicator Phenol red Mycoplasma Detection Negative Sterility Tested Sterile filtered using 0.22 µm filter Form Liquid Shipping Condition Room temperature Eagle’s Minimal Essential Medium (EMEM)
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box_shadow_style="" z_index_subgroup="regular" z_index="" z_index_hover="" overflow="" background_type="single" gradient_start_color="" gradient_end_color="" gradient_start_position="0" gradient_end_position="100" gradient_type="linear" radial_direction="center center" linear_angle="180" background_color="" background_image="" background_image_id="" lazy_load="none" skip_lazy_load="" background_position="left top" background_repeat="no-repeat" background_blend_mode="none" render_logics="" sticky="off" sticky_devices="small-visibility,medium-visibility,large-visibility" sticky_offset="" absolute="off" absolute_props="" filter_type="regular" filter_hover_element="self" filter_hue="0" filter_saturation="100" filter_brightness="100" filter_contrast="100" filter_invert="0" filter_sepia="0" filter_opacity="100" filter_blur="0" filter_hue_hover="0" filter_saturation_hover="100" filter_brightness_hover="100" filter_contrast_hover="100" filter_invert_hover="0" filter_sepia_hover="0" filter_opacity_hover="100" filter_blur_hover="0" transform_type="regular" transform_hover_element="self" transform_scale_x="1" transform_scale_y="1" transform_translate_x="0" transform_translate_y="0" transform_rotate="0" transform_skew_x="0" transform_skew_y="0" transform_scale_x_hover="1" transform_scale_y_hover="1" transform_translate_x_hover="0" transform_translate_y_hover="0" transform_rotate_hover="0" transform_skew_x_hover="0" transform_skew_y_hover="0" transform_origin="" transition_duration="300" transition_easing="ease" transition_custom_easing="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset="" last="true" border_position="all" first="true"][fusion_text columns="" column_min_width="" column_spacing="" rule_style="" rule_size="" rule_color="" hue="" saturation="" lightness="" alpha="" content_alignment_medium="" content_alignment_small="" content_alignment="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" sticky_display="normal,sticky" class="" id="" margin_top="" margin_right="" margin_bottom="" margin_left="" fusion_font_family_text_font="" fusion_font_variant_text_font="" font_size="" line_height="" letter_spacing="" text_transform="" text_color="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset=""]Eagle’s Minimum Essential Medium (EMEM)
Background
Eagle's Minimal Essential Medium (EMEM) developed by Harry Eagle and is widely used. It is composed of necessary salts for optimal cell growth and maintenance. Additionally, it contains high glucose, essential amino acids, and vitamins. Ever since, several versions of EMEM have been developed by modification of its composition. This media has is suitable for a wide range of mammalian cells, adhesive as well as suspension cells.Ingridients
Moreover, PurMa TM EMEM Medium contains:- Low level of glucose (1.00 g/ liter)
- Sodium bicarbonate (1.5 g/L)
- Additionally, PurMa Biologics also offers EMEM Medium with high sodium bicarbonate (2.438 g/L) (Cat # P3S032110)
- 2 mM L-glutamine (292 mg/L)
- 1mM sodium pyruvate (0.11g/L)
- Additionally, a higher concentration of amino acids, vitamins, inositol and choline are present in this medium.
Why PurMa Biologics? Why we stand behind our cell culture media
Our products are the outcome of 30 years of experience in cutting edge cell and tissue culture science. We routinely manufacture over 1500 media which is the most comprehensive collection of cell and tissue culture media. We utilize %99 pure amino acids. This will significantly increase the consistency among your experiments, and the result of your lab compared to other laboratories working on the same issues. Considering the highest quality, our prices are extremely reasonable.Application of EMEM
Additionally, this media has elements that prevent clumping. This property certainly makes this media suitable for growing tumor cells as well as hybridoma cells lines. The dedicated scientists in PurMa Biologics have decades of experience with this media for culturing primary as well as commercial cell lines including human Osteosarcoma, Hek293 Cell, HeLa, Jurkat, MG63 cells, MCF-7, PC12, PBMC, and hybridoma cells.Formulation
for complete formulation click here: EMEM Formultation References- Effects of assay medium composition on macrophage-mediated tumor cell binding and lysis. Hasday et al. J Immunol Methods. 1988 Nov 10;114(1-2):243-52. doi: 10.1016/0022-1759(88)90180-9. PMID: 3141516.
- Comparative activity in different media of ketoconazole, miconazole and amphotericin B against Candida lusitaniae and sucrose-negative Candida tropicalis. Mignini et al. Chemioterapia. 1988 Feb;7(1):38-41. PMID: 3378274.
- Dextran T-500 improves survival and spreading of chick embryo cells in serum-free medium. Pietrzkowski et al. Folia Histochem Cytobiol. 1988;26(3):123-31. PMID: 2461881.
Parameter Specification Appearance Red, clear liquid pH 7.2 ± 0.1 Osmolality 275-360 mOsm/L Endotoxin NMT< 2EU/mL Mycoplasma Negative Suitability Suitable for mammalian cell culture Additive Sodium pyruvate Indicator Phenol red Mycoplasma Detection Negative Sterility Tested Sterile filtered using 0.22 µm filter Form Liquid Shipping Condition Room temperature Leibovitz’s L-15 Medium – MAX
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box_shadow_style="" z_index_subgroup="regular" z_index="" z_index_hover="" overflow="" background_type="single" gradient_start_color="" gradient_end_color="" gradient_start_position="0" gradient_end_position="100" gradient_type="linear" radial_direction="center center" linear_angle="180" background_color="" background_image="" background_image_id="" lazy_load="none" skip_lazy_load="" background_position="left top" background_repeat="no-repeat" background_blend_mode="none" render_logics="" sticky="off" sticky_devices="small-visibility,medium-visibility,large-visibility" sticky_offset="" absolute="off" absolute_props="" filter_type="regular" filter_hover_element="self" filter_hue="0" filter_saturation="100" filter_brightness="100" filter_contrast="100" filter_invert="0" filter_sepia="0" filter_opacity="100" filter_blur="0" filter_hue_hover="0" filter_saturation_hover="100" filter_brightness_hover="100" filter_contrast_hover="100" filter_invert_hover="0" filter_sepia_hover="0" filter_opacity_hover="100" filter_blur_hover="0" transform_type="regular" transform_hover_element="self" transform_scale_x="1" transform_scale_y="1" transform_translate_x="0" transform_translate_y="0" transform_rotate="0" transform_skew_x="0" transform_skew_y="0" transform_scale_x_hover="1" transform_scale_y_hover="1" transform_translate_x_hover="0" transform_translate_y_hover="0" transform_rotate_hover="0" transform_skew_x_hover="0" transform_skew_y_hover="0" transform_origin="" transition_duration="300" transition_easing="ease" transition_custom_easing="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset="" last="true" border_position="all" first="true"][fusion_text columns="" column_min_width="" column_spacing="" rule_style="" rule_size="" rule_color="" hue="" saturation="" lightness="" alpha="" content_alignment_medium="" content_alignment_small="" content_alignment="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" sticky_display="normal,sticky" class="" id="" margin_top="" margin_right="" margin_bottom="" margin_left="" fusion_font_family_text_font="" fusion_font_variant_text_font="" font_size="" line_height="" letter_spacing="" text_transform="" text_color="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset=""]Leibovitz’s L-15 Medium - MAX
Background
Leibovitz’s L-15 Medium - MAX is one of the commonly used Media for Mammalian cell culture.Advantages
The advantages of Leibovitz’s L-15 MAX over Leibovitz’s L-15 are:
- Higher PH Capacity
- Moreover, it contains 4 mM L-Alanyl-L-Glutamine (0.869 g/L) instead of L-Glutamine and therefore:
- Possess longer shelf life.
- More importantly, minimizes toxic ammonia build-up.
- Significantly improves cell viability and growth.
- Remains stable across a wide range of temperatures.
- Improves the culture of primary cells.
Mechanism
Although L-glutamine is a vital amino acid, it degrades, generating toxic ammonia and pyrrolidine carboxylic acid. Therefore, one way to minimize L-glutamine degradation in media is to gradually add these amino acids to your cell culture. Unfortunately, maintaining a consistent level of L-glutamine by tracking time can be a tedious and potentially impossible task. Alternatively, L-alanyl-L-glutamine can be used since it is much more stable in aqueous solutions. More importantly, it does not easily degrade and gradually releases aminopeptidases. As a result, L-glutamine and L-alanine are used by the cells for protein production in the TCA cycle. In addition to our Max formulation products, we manufacture PurMaTM GluaSup (Cat# P3S210 559) is a combination of L-alanyl-L-glutamine that prevents the degradation of L-glutamine.Contents
Leibovitz’s L-15 Medium - MAX contains:
- 5 mM sodium pyruvate
- High glucose (4.5g/L)
- Non-Essential Amino Acids
- 3.7 g/L Sodium Bicarbonate; PurMa Biologics Manufactures several types of Leibovitz’s L-15 MAX based on the amount of Sodium Bicarbonate and HEPES (PH capacity)
- Phenol Red
Formulation
The complete formulation is here: Leibovitz's L-15 MAX FormulationReferences
- The effects of insulin, transferrin and androgens on rat prostate explants in serum-free organ culture. Nguyen-Le XK, Brière N, Corcos J. 1997;6(3):339-49. doi: 10.1002/biof.5520060304. PMID: 9288404.
- Expression of differentiation molecules is preserved in human fetal kidneys during culture. Brière N, Droz D.Acta Histochem. 1990;89(2):157-66. doi: 10.1016/s0065-1281(11)80351-x.PMID: 2093265
Parameter Specification Appearance Red, clear liquid pH 7.2 ± 0.1 Osmolality 275-360 mOsm/L Endotoxin NMT< 2EU/mL Mycoplasma Negative Suitability Suitable for mammalian cell culture Additive Sodium pyruvate Indicator Phenol red Mycoplasma Detection Negative Sterility Tested Sterile filtered using 0.22 µm filter Form Liquid Shipping Condition Room temperature Minimum Essential Media (MEM) In Hanks’ Buffer
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Background
Minimum Essential Media (MEM) In Hanks’ Buffer is a modification of Basal Medium Eagle . It was originally developed by Harry Eagle for specific requirements of certain subtypes. Additionally, it includes higher concentrations of amino acids which is more suitable for cultured mammalian cells. Moreover, PurMa Biologics manufactures MEM with Earle’s buffer for use in a CO2 incubator, or with Hanks' salts for use without CO2.PurMa™ MEM media in Hank’s buffer includes Hank's buffer
Hanks buffer, it contains:- Potassium Chloride
- Potassium Phosphate, monobasic
- Sodium Bicarbonate
- Sodium Chloride
- Sodium Phosphate, dibasic
Additionally, MEM in Hanks’ Buffer contains:
- Low level of glucose (1.00 g/ liter)
- Sodium bicarbonate (0.35 g/L). If , however, higher than 5% CO2 is used, a higher % of sodium bicarbonate is needed.
- 2 mM L-glutamine (292 mg/L)
Why PurMa Biologics? Why we stand behind our cell culture media
Our products are the outcome of 30 years of experience in cutting edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. Moreover, We utilize %99 pure amino acids. This will significantly increase the consistency among your experiments. Considering the highest quality, our prices certainly are extremely reasonable.Applications
MEM has been used for the cultivation of a wide variety of cells grown in monolayers attached or in suspension cell lines such as HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts as well as primary rat astrocytes. MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% fetal bovine serum (FBS).Formulation
Complete formulation click here as well as in Formulation Tab: MEM (HBSS) Formulation in Hanks' BufferReferences
- Culture of granulosa cells in collagen gels: the influence of cell shape on steroidogenesis. Carnegie et al. Biol Reprod. 1988 May;38(4):881-90. doi: 10.1095/biolreprod38.4.881. PMID: 3401543
- Myelin formation in rat dorsal root ganglion cultured in a serum-free medium. Ninomiya et al. J Anat. 1987 Jun;152:209-13. PMID: 3654371
Parameter Specification Appearance Red, clear liquid pH 7.2 ± 0.1 Osmolality 275-360 mOsm/L Endotoxin NMT< 2EU/mL Mycoplasma Negative Suitability Suitable for mammalian cell culture Additive N/A Indicator Phenol red Mycoplasma Detection Negative Sterility Tested Sterile filtered using 0.22 µm filter Form Liquid Shipping Condition Room temperature Title
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