Advanced MEM-Alpha with Nucleosides in Hanks’ Buffer

Advanced MEM-Alpha with Nucleosides in Hanks’ Buffer


Advanced MEM-Alpha with Nucleosides in Hanks’ Buffer contains L-Alanyl-L-Glutamine as well as ingredients to allow for serum reduction.  Additionally, A-MEM-α-N needs 2-5% FBS and provides more consistency.


Advanced MEM-Alpha with Nucleosides in Hanks’ Buffer (A-MEM-α-N-HBSS)


PurMaTM Advanced MEM-Alpha with Nucleosides in Hanks’ Buffer (A-MEM-α-N-HBSS) contains Nucleosides as well as components that allow for a 60–90% reduction in FBS supplementation. More importantly, using synthetic components such as recombinant insulin, albumin, and transferrin increase consistency. This results in less variation amongst lot-to-lot serum changes. In A-MEM-α-N, L-Alanyl-L-Glutamine is used as the source of glutamine. Additionally, reducing FBS requirements lowers the cost of cell culture experiments.


Advanced MEM-α-N in HBSS Contains:

  • includes all twenty essential and non-essential amino acids with higher concentrations than regular and MAX formulations.
  • A-MEM-α-N incorporates elements that significantly reduce the need for a serum for cell culture.

Crucial Ingredients:

  • PurMaTM Human Transferrin (Holo): prevents Improper iron storage and delivery in cell culture systems which is a major contributor to oxidative stress and protein damage.
  • PurMaTM Insulin Recombinant
  • Trace of other elements which mimic the elements in fetal bovine serum.

PurMaTM Advanced MEM-α with nucleoside (A-MEM-α-N) is modified from Advanced MEM-α with the following ingredients:

  • Ribonucleosides
  • Deoxyribonucleosides

Consequently, Advanced MEM-α with Nucleosides in Hanks’ Buffer (A-MEM-α-N-HBSS) is a significant step toward a chemically defined form of this media. Unquestionably, Chemically Defined Advanced MEM-α-N (CD- A-MEM-α-N) is the outcome of decades of cutting-edge research and will soon be offered globally to laboratories that are looking for consistency and repeatability in their data.


Complete formulation is available here: Adnaced MEM Alpha with Nucleoside in HBSS buffer Formulation


  1. A previously functional tetracycline-regulated transactivator fails to target gene expression to the bone. Schmidt E, Eriksson M. BMC Res Notes. 2011 Aug 11;4:282. doi: 10.1186/1756-0500-4-282. PMID: 21835026
  2. The growth of L-cells and Vero cells on an autoclavable MEM-peptone medium. L Keay Biotechnology and bioengineering, 19(3), 399-411 (1977-03-01).
  3. Minimum essential medium alpha (MEM) enhances assisted reproductive technology results. I. Mouse embryo study. Wun WS, et al. Assist Reprod Genet. 1994 Jul;11(6):303-7. doi: 10.1007/BF02215717. PMID: 7734915.
Parameter Specification
Appearance Red, clear liquid
pH 7.2 ± 0.1
Osmolality 275-360 mOsm/L
Endotoxin NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive Sodium pyruvate
Indicator Phenol red
Mycoplasma Detection Negative
Sterility Tested Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature


Advanced-MEM-α-N with Hanks’ Buffer (HBSS) Formulation


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