MES Stock Buffer Solution

Description

Background

MES Stock Buffer Solution is one of the commonly used buffering agents in cell culture, biology, and biochemistry. It, however, does not form complexes with most of the metals used in environmental and biological studies. It easily dissolves in water and has minimum lipid solubility, making it impermeable to membranes. Moreover, MES buffer induces autophagy in cells. We also produce this widely used buffer in various pH formats.

 

Features

• Cell Culture Suited as well as molecular biology suited.
• Provides a buffer solution to maintain a medium in physiological pH (7.2-7.6).
• PurMa Biologics manufactures this buffer free of  Mg²⁺ and Ca ²⁺
• Free of RNAse /DNase
• Free of Proteases. It is rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity.
• We rigorously test for contamination of endotoxin and mycoplasma.
• PurMa Biologics manufactures this buffer in several pH formats.
• We sterilize it by 0.2µm filter.
• PurMa Biologics packs all the buffers under N₂, so the pH does not change over time before opening the bottle.

Application

It plays a significant part in the preparation of Murashige and Skoog growth medium for the growth of cannabis in cell culture. In molecular biology, MES is commonly used in the conjugation of hybridization probes to beads before PCR amplification.

 

PurMa Biologics Manufactures Several Grades of Buffers

Regular Grade Buffer

PurMa Biologics manufactures Regular grade of buffer by using:  PurMa™ Cell Culture Suited Water (Cat# P3W110101)

We sterilize the buffers by autoclaving the solution. Finally we pack the buffer in our Class 100 Aseptic Facility.

Cell Culture Suited Grade Buffer

PurMa Biologics manufactures this grade of buffer by using :PurMa™ Cell Culture Suited Water (Cat# P3W110101)

After adjusting the pH, we finally, sterilize the buffer by  3 times passing through 0.2 µm filters. Additionally,  We pack the buffer under N2 gas  in our Class 100 Aseptic Facility.

RNase and DNase Free Grade Buffer

PurMa Biologics manufactures this grade of buffer by using :PurMa™ Ultra-Pure Cell Culture Suited Water (Cat# P3W110102)

We sterilize the buffers by autoclaving the solution.  Additionally, we pass the buffer  three times through 0.1 µm filters,  and finally  pack it under N2 gas in our Class 100 Aseptic Facility.

Cell Culture Suited / Endotoxin Depleted Grade Buffer

PurMa Biologics manufactures this grade of buffer by using: PurMa™ Ultra-Pure Cell Culture Suited Water (Cat# P3W110102)

After depleting  the endotoxins (see below for more details), we sterilize the buffer by 3 times passing through  0.1 µm filters and pack under N2 gas in our Class 100 Aseptic Facility

The Endotoxin Removing Procedure from Buffer

PurMa Biologics has the most sophisticated endotoxin removal procedure. To remove this pathogen, we pass the buffer  through the PurMa™ Endotoxin Elimination Column (Cat# P6H1216251). Furthermore, in our proprietary column,  we covalently conjugate Sepharose 6B  to an endotoxin-specific absorbent. We finally  eliminate the endotoxin residual by passing the unbound fraction three times through 0.1µm filters.

References

1. Hydrogen ion buffers for biological research. Good NE, Winget GD, Winter W, Connolly TN, Izawa S, Singh RM. Biochemistry. 1966 Feb;5(2):467-77. doi: 10.1021/bi00866a011. PMID: 5942950.
2. Noncomplexing Tertiary Amines as “Better” Buffers Covering the Range of pH 3-11. Temperature Dependence of Their Acid Dissociation Constants. Kandegedara A, Rorabacher DB. Anal Chem. 1999 Aug 1;71(15):3140-4. doi: 10.1021/ac9902594. PMID: 21662904.