Dulbecco’s Phosphate Buffered Saline (DPBS)

Description

Background

Dulbecco’s Phosphate Buffered Saline (DPBS) is a balanced salt solution. It is particularly used to maintain viability of cells for a short time instead of promoting their growth. During interval period we maintain osmotic balance and physiological pH to protect cellular integrity. So, DPBS serves as an isotonic saline solution or buffer for washing cells and tissues like in chromatographic procedures where incubation is not necessary.

General Feature

• PurMa Biologics manufactures this buffer in two pH: 7.4 ± 0.01 and 7.2 ± 0.01 at 25°C.
• We rigorously sterilize it by 0.22µm membrane.
• Additionally, it is a cost-effective balanced salt solution used for a variety of biochemical applications.
• PurMa Biologics packs all our buffer under N_2, as a result, the pH won’t change over time before opening the bottle.

Specific Feature

• Cell Culture Suited
• We deplete  Mg²⁺ and Ca²⁺ ions
• Our manufacturing process removes RNase/DNase
• Free of Proteases.
• PurMa Biologics  rigorously test for contaminating,  nonspecific endonuclease, exonuclease, as well as  RNase activity.

For complete formulation, Click here:DPBS Formulation

PurMa Biologics Manufactures Several Grades of Buffers

Regular Grade Buffer

PurMa Biologics manufactures Regular grade of buffer by using:  PurMa™ Cell Culture Suited Water (Cat# P3W110101)

We sterilize the buffers by autoclaving the solution. Finally we pack the buffer in our Class 100 Aseptic Facility.

Cell Culture Suited Grade Buffer

PurMa Biologics manufactures this grade of buffer by using :PurMa™ Cell Culture Suited Water (Cat# P3W110101)

After adjusting the pH, we finally, sterilize the buffer by  3 times passing through 0.2 µm filters. Additionally,  We pack the buffer under N2 gas  in our Class 100 Aseptic Facility.

RNase and DNase Free Grade Buffer

PurMa Biologics manufactures this grade of buffer by using :PurMa™ Ultra-Pure Cell Culture Suited Water (Cat# P3W110102)

We sterilize the buffers by autoclaving the solution.  Additionally, we pass the buffer  three times through 0.1 µm filters,  and finally  pack it under N2 gas in our Class 100 Aseptic Facility.

Cell Culture Suited / Endotoxin Depleted Grade Buffer

PurMa Biologics manufactures this grade of buffer by using: PurMa™ Ultra-Pure Cell Culture Suited Water (Cat# P3W110102)

After depleting  the endotoxins (see below for more details), we sterilize the buffer by 3 times passing through  0.1 µm filters and pack under N2 gas in our Class 100 Aseptic Facility

The Endotoxin Removing Procedure from Buffer

PurMa Biologics has the most sophisticated endotoxin removal procedure. To remove this pathogen, we pass the buffer  through the PurMa™ Endotoxin Elimination Column (Cat# P6H1216251). Furthermore, in our proprietary column,  we covalently conjugate Sepharose 6B  to an endotoxin-specific absorbent. We finally  eliminate the endotoxin residual by passing the unbound fraction three times through 0.1µm filters.

 References:

1.  Plaque formation and isolation of pure lines with poliomyelitis viruses. Dulbecco et al.J Exp Med.; 1954 Jan 31 99(2): 167–182. doi: 10.1084/jem.99.2.167. PMID: 13130792.
2. Deep freezing of sheep embryos. Willadsen Set al. J Reprod Fertil. 1976 Jan;46(1):151-4. doi: 10.1530/jrf.0.0460151. PMID: 1271336.

 

Learn more: PBS vs DPBS