Dulbecco's Phosphate Buffered Saline (DPBS) is a balanced salt solution. It is particularly used to maintain viability of cells for a short time instead of promoting their growth. It maintain osmotic balance and physiological pH during interval period to protect cellular integrity.
Earle's Balanced Salt Solution (EBSS) is an isotonic buffer solution. It is however, composed of inorganic salts and carbohydrates as an energy source designed for the short-term maintenance of mammalian cells in a CO2 environment.
Hanks’ Balanced Salt Solution (HBSS) contains inorganic salts and glucose. Moreover, HBSS for a short time provides an ideal environment to preserve the structural and physiological integrity of cells in vitro. Additionally, HBSS maintains a balance between intra and extracellular osmolarity.
HEPES Stock Buffer Solution adjusts the pH in cell cultures as well as biochemical analysis. HEPES Maintains psychological pH despite changes in carbon dioxide concentration, therefore HEPES it acts better than bicarbonate in cell culture.