- ✓Proudly made in the USA, manufactured in house
- ✓Certificate of Analysis available for every lot
- ✓Backed by decades of cell culture and protein biochemistry expertise
- ✓Bulk pricing, purchase orders, and custom formulations welcome
HEPES Stock Buffer Solution
A HEPES stock buffer that holds physiological pH in cell culture and biochemical work.
- Maintains stable pH despite changes in CO2, outperforming bicarbonate
- For cell culture and biochemical analysis
Description
Background
HEPES Stock Buffer Solution is one of the commonly used buffers in cell cultures as well as biochemical analysis. HEPES maintains physiological pH despite changes in carbon dioxide concentration, therefore it acts better than bicarbonate in cell culture applications. However, HEPES shows an unwanted photochemical process in the presence of riboflavin when exposed to ambient light to produce hydrogen peroxide. We therefore recommend keeping the mentioned solutions in the dark.
Features
- Cell Culture Suited
- Provides a buffer solution to maintain the physiological pH despite changes in carbon dioxide concentration.
- PurMa Biologics manufactures this buffer using Mg2+ and Ca 2+free water
- Free of RNAse /DNase
- Free of Proteases. It is rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity.
- We rigorously test for contamination of endotoxin and mycoplasma.
- PurMa Biologics manufactures this buffer in several PH formats and NaOH adjusts the pH.
- We Sterilize this buffer using 0.2µm filters.
- As it is packed under N2, the pH won’t change over time before opening the bottle.
PurMa Biologics Manufactures Several Grades of Buffers
Regular Grade Buffer
PurMa Biologics manufactures Regular grade of buffer by using: PurMa™ Cell Culture Suited Water (Cat# P3W110101)
We sterilize the buffers by autoclaving the solution. Finally we pack the buffer in our Class 100 Aseptic Facility.
Cell Culture Suited Grade Buffer
PurMa Biologics manufactures this grade of buffer by using :PurMa™ Cell Culture Suited Water (Cat# P3W110101)
After adjusting the pH, we finally, sterilize the buffer by 3 times passing through 0.2 µm filters. Additionally, We pack the buffer under N2 gas in our Class 100 Aseptic Facility.
RNase and DNase Free Grade Buffer
PurMa Biologics manufactures this grade of buffer by using :PurMa™ Ultra-Pure Cell Culture Suited Water (Cat# P3W110102)
We sterilize the buffers by autoclaving the solution. Additionally, we pass the buffer three times through 0.1 µm filters, and finally pack it under N2 gas in our Class 100 Aseptic Facility.
Cell Culture Suited / Endotoxin Depleted Grade Buffer
PurMa Biologics manufactures this grade of buffer by using: PurMa™ Ultra-Pure Cell Culture Suited Water (Cat# P3W110102)
After depleting the endotoxins (see below for more details), we sterilize the buffer by 3 times passing through 0.1 µm filters and pack under N2 gas in our Class 100 Aseptic Facility
The Endotoxin Removing Procedure from Buffer
PurMa Biologics has the most sophisticated endotoxin removal procedure. To remove this pathogen, we pass the buffer through the PurMa™ Endotoxin Elimination Column (Cat# P6H1216251). Furthermore, in our proprietary column, we covalently conjugate Sepharose 6B to an endotoxin-specific absorbent. We finally eliminate the endotoxin residual by passing the unbound fraction three times through 0.1µm filters.
References
- Toxicity of light-exposed Hepes media. Lepe-Zuniga JL, Zigler JS Jr, Gery I. J Immunol Methods. 1987 Oct 23;103(1):145. doi: 10.1016/0022-1759(87)90253-5. PMID: 3655381.
- Analysis of the cytotoxic effects of light-exposed HEPES-containing culture medium. Zigler, JS Jr , Lepe-Zuniga JL, Vistica B, Gery I. In Vitro Cell Dev Biol. 1985 May;21(5):282-7. doi: 10.1007/BF02620943. PMID: 4019356.
| Parameter | Specification |
|---|---|
| Appearance | Red, clear liquid |
| pH | 6.5 ± 0.1; 7.0 ± 0.1; 7.5 ± 0.1; 7.6 ± 0.1; 8.0 ± 0.1; 8.5 ± 0.1; 9.0 ± 0.1 |
| Osmolality | 275-360 mOsm/L |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Suitability | Suitable for mammalian cell culture |
| Additive | N/A |
| Indicator | N/A |
| Mycoplasma Detection | Negative |
| Sterility Tested | Sterile filtered using 0.22 µm filter |
| Form | Liquid |
| Shipping Condition | Room temperature |
Learn more: What Is HEPES Buffer?
Additional information
| Concentration | 0.1 M, 0.2 M, 0.5 M, 1 M, 2 M |
|---|---|
| Grade | Regular, RNase and DNase Free, Cell Culture Suited, Ca and Mg Free, Cell Culture Suited, Ca and Mg Free, Endotoxin Depleted |
| pH | pH 6.5, pH 7.0, pH 7.5, pH 7.6, pH 8.0, pH 8.5, pH 9.0 |
| Size | 1 x 100 ml, 6 x 100 ml, 1 x 500 ml, 6 x 500 ml |
Documents
Certificate of Analysis (COA): Available upon request for your specific order — email info@purmabiologics.com and we will send the current lot documentation.
Safety Data Sheet (SDS): Available upon request — email info@purmabiologics.com.
Frequently asked questions
What sizes and pack quantities does HEPES buffer come in?
HEPES Stock Buffer Solution is supplied in 1 x 100 mL, 6 x 100 mL, 1 x 500 mL and 6 x 500 mL.
Which molarities and grades of HEPES do you supply?
HEPES is available at 0.1 M, 0.2 M, 0.5 M, 1 M and 2 M, in Regular, RNase and DNase Free, Cell Culture Suited, calcium and magnesium free, and endotoxin depleted grades.
Which pH options are available for HEPES buffer?
HEPES is offered at pH 6.5, 7.0, 7.5, 7.6, 8.0, 8.5 and 9.0, covering the full range it buffers usefully.
Why add HEPES to cell culture medium?
HEPES holds pH steady when cells spend time outside a CO2 incubator, during imaging, sorting or extended handling at the bench. It is normally added at 10 to 25 mM. Note that HEPES can increase light sensitivity in some media, so protect from light where that matters.
Can I order HEPES buffer in bulk or on a purchase order?
Yes. Custom formulations, non standard fill volumes and bulk orders are all available, since everything is made in house. Purchase orders are accepted and bulk pricing is available. Request a quote from this page and we will come back with pricing and lead time.
Related products
-
- Sale!
- Cell Culture Media, Eagle's Minimal Essential Medium (EMEM)
Eagle’s Minimal Essential Medium-MAX (EMEM-MAX)
- Price range: $29.15 through $547.30
- Select options This product has multiple variants. The options may be chosen on the product page
-
- Sale!
- Cell Culture Media, Roswell-Park Memorial Institute 1640
PurMa™ Roswell Park Memorial Institute 1640 (RPMI-1640)
- Price range: $21.70 through $549.37
- Select options This product has multiple variants. The options may be chosen on the product page
-
- Sale!
- Cell Culture Suited Buffers
Earle’s Balanced Salt Solution (EBSS)
- Price range: $21.95 through $397.89
- Select options This product has multiple variants. The options may be chosen on the product page
Reviews
There are no reviews yet.