TRIS Buffer Solution

Description

Background

PurMa™ TRIS Buffer Solution is a frequently used buffer in molecular biology, cell culture and protein biochemistry. In fact, it provides a buffering capacity  in solutions. Moreover, PurMa™ TRIS Buffer Solution has an important impact on nucleic acid applications. It contains a primary amine and thus undergoes the reactions associated with typical amines, e.g., condensations with aldehydes. Additionally, the pH of tris buffer has an important impact on its function.

Features

• It has an important role in molecular biology as well as Cell Culture.
• Free of  Mg²⁺ and Ca ²⁺ 
• Free of RNAse /DNase
• Free of Proteases. It is rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity.
• It is rigorously tested for contamination of endotoxin and mycoplasma.
• It is manufactured in several pH formats.
• Sterile filtered by 0.1µm.
• It is packed under N₂, so the pH won’t change over time before opening the bottle.

PurMa Biologics Manufactures Several Grades of Buffers

Regular Grade Buffer

PurMa Biologics manufactures Regular grade of buffer by using:  PurMa™ Cell Culture Suited Water (Cat# P3W110101)

We sterilize the buffers by autoclaving the solution. Finally we pack the buffer in our Class 100 Aseptic Facility.

Cell Culture Suited Grade Buffer

PurMa Biologics manufactures this grade of buffer by using :PurMa™ Cell Culture Suited Water (Cat# P3W110101)

After adjusting the pH, we finally, sterilize the buffer by  3 times passing through 0.2 µm filters. Additionally,  We pack the buffer under N2 gas  in our Class 100 Aseptic Facility.

RNase and DNase Free Grade Buffer

PurMa Biologics manufactures this grade of buffer by using : PurMa™ Ultra-Pure Cell Culture Suited Water (Cat# P3W110102)

We sterilize the buffers by autoclaving the solution.  Additionally, we pass the buffer  three times through 0.1 µm filters,  and finally  pack it under N2 gas in our Class 100 Aseptic Facility.

Cell Culture Suited / Endotoxin Depleted Grade Buffer

PurMa Biologics manufactures this grade of buffer by using: PurMa™ Ultra-Pure Cell Culture Suited Water (Cat# P3W110102)

After depleting  the endotoxins (see below for more details), we sterilize the buffer by 3 times passing through  0.1 µm filters and pack under N2 gas in our Class 100 Aseptic Facility

The Endotoxin Removing Procedure from Buffer

PurMa Biologics has the most sophisticated endotoxin removal procedure. To remove this pathogen, we pass the buffer  through the PurMa™ Endotoxin Elimination Column (Cat# P6H1216251). Furthermore, in our proprietary column,  we covalently conjugate Sepharose 6B  to an endotoxin-specific absorbent. We finally  eliminate the endotoxin residual by passing the unbound fraction three times through 0.1µm filters.

References

1. The structure of crystalline tris: a plastic crystal precursor, buffer, and acetylcholine attenuator. Rudman R, Eilerman D, La Placa SJ. Science. 1978 May 5;200(4341):531-3. doi:10.1126/ science. 644311. PMID: 644311.
2. Interactions of tris buffer with nucleotides: the crystal structure of tris(hydroxymethyl) methylammonium adenosine 5′-diphosphate dihydrate. Shakked Z, Viswamitra MA, Kennard O. Biochemistry. 1980 Jun 10;19(12):2567-71. doi: 10.1021/bi00553a005. PMID: 7397089.