EDTA Buffer Solution

Description

Background

PurMa Biologics manufactures various grades of PurMa EDTA Buffer Solution NaEDTA•2HO using different grades of water ( see below). Moreover, EDTA solution is suitable for biochemistry or molecular biology applications requiring a chelator of divalent metal ions. Additionally, we adjust the pH to 8.0 with sodium hydroxide.

Features

• Cell Culture Suited.
• provides a  cost-effective and easy to use buffer.
• Suitable for a variety of biochemical applications.
• EDTA  chelates  heavy metals such as Mg²⁺ and Ca ²⁺.
• PurMa Biologics manufactures EDTA in Mg²⁺ and Ca ²⁺-free water.
• Free of RNAse /DNase
• Free of Proteases. It is rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity.
• It is rigorously tested for contamination of endotoxin and mycoplasma.
• Is manufactured in two pH formats: pH: 8.00 ± 0.01 and 8.5 ± 0.01 at 25°C.
• Sterile filtered by 0.22µm membrane (in case of endotoxin depleted HBSS by 0.1µm)
• It is packed under N_2; therefore, the pH won’t change over time before opening the bottle.

Formulation

For complete formulation, click here:EDTA Formulation

PurMa Biologics Manufactures Several Grades of Buffers

Regular Grade Buffer

PurMa Biologics manufactures Regular grade of buffer by using:  PurMa™ Cell Culture Suited Water (Cat# P3W110101)

We sterilize the buffers by autoclaving the solution. Finally we pack the buffer in our Class 100 Aseptic Facility.

Cell Culture Suited Grade Buffer

PurMa Biologics manufactures this grade of buffer by using :PurMa™ Cell Culture Suited Water (Cat# P3W110101)

After adjusting the pH, we finally, sterilize the buffer by  3 times passing through 0.2 µm filters. Additionally,  We pack the buffer under N2 gas  in our Class 100 Aseptic Facility.

RNase and DNase Free Grade Buffer

PurMa Biologics manufactures this grade of buffer by using :PurMa™ Ultra-Pure Cell Culture Suited Water (Cat# P3W110102)

We sterilize the buffers by autoclaving the solution.  Additionally, we pass the buffer  three times through 0.1 µm filters,  and finally  pack it under N2 gas in our Class 100 Aseptic Facility.

Cell Culture Suited / Endotoxin Depleted Grade Buffer

PurMa Biologics manufactures this grade of buffer by using: PurMa™ Ultra-Pure Cell Culture Suited Water (Cat# P3W110102)

After depleting  the endotoxins (see below for more details), we sterilize the buffer by 3 times passing through  0.1 µm filters and pack under N2 gas in our Class 100 Aseptic Facility

The Endotoxin Removing Procedure from Buffer

PurMa Biologics has the most sophisticated endotoxin removal procedure. To remove this pathogen, we pass the buffer  through the PurMa™ Endotoxin Elimination Column (Cat# P6H1216251). Furthermore, in our proprietary column,  we covalently conjugate Sepharose 6B  to an endotoxin-specific absorbent. We finally  eliminate the endotoxin residual by passing the unbound fraction three times through 0.1µm filters.

References

1. Purification of two endonucleases from potato tubers. BJOERK W. Biochim Biophys Acta. 1965 Apr 19;95:652-62. PMID: 14324816.
2. Chelating agents. Bergan T, Klaveness J, Aasen AJ. Chemotherapy. 2001 Jan-Feb;47(1):10-4. doi: 10.1159/000048495. PMID: 11125227.