- ✓Proudly made in the USA, manufactured in house
- ✓Certificate of Analysis available for every lot
- ✓Backed by decades of cell culture and protein biochemistry expertise
- ✓Bulk pricing, purchase orders, and custom formulations welcome
Ultra-Pure Cell Culture Suited Water
PurMa™ Ultra-Pure Cell Culture Suited Water our most specialized water and undoubtedly holds the highest standards for a cell culture water.
Description
Background
PurMa™ Ultra-Pure Cell Culture Suited Water is our most specialized water and undoubtedly holds the highest standards for cell culture water.
This water does not contain
- Trace of organic and non-organic compounds.
- Dissolved and particulate matter of any size.
- Volatile and non-volatile
- Reactive, and inert
- Hydrophilic and hydrophobic
- Dissolved gases.
Special Features
- PurMa Biologics purifies Ultra-Pure Cell Culture Suited Water by Deionization (DI) and additionally followed by Reverse Osmosis process (18.2 ΩM/cm2).
- Moreover, we deplete traces of heavy metals including Ca+2 and Mg +2 using ion exchange chromatography resins.
- Additionally, it goes through Double-Distillation.
- Sterilized by 0.1 µm filters.
- we pack all types of our water under N2.
Important Note: Do NOT expose this water to open air as it absorbs traces of various ions including bicarbonate.
References
- Water on hydrophobic surfaces: Mechanistic modeling of hydrophobic interaction chromatography. Wang et al. J. J Chromatogr A. 2016 Sep 23;1465:71-8. . Epub 2016 Jul 30.
- A Review on Reverse Osmosis and Nanofiltration Membranes for Water Purification. Yang et al. Polymers (Basel). 2019 Jul 29;11(8):1252.
| General specifications | |
| Parameter | Specification |
| Grade | Ultra-Pure Cell Culture Suited |
| Appearance | Clear |
| pH | 5-7 |
| Osmolality | 275-360 mOsm/L |
| Filtration | Sterile filtered using 0.1 µm filter |
| Indicator | NA |
| Conductivity (Measured) | 0.056 µs/cm2 |
| Resistancy (Calculated) | 18.2 Ωm |
| Shipping Condition | Ambient |
| Refractive index | n20/D 1.34 (lit.) |
| Molecular Biology Parameters | |
| DNases & RNases | NOT Detected |
| proteases | NOT Detected |
| phosphatases | NOT Detected |
| Pathogens Evaluation | |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Mold & Fungi/Yeast | Negative |
| Bacteria | Negative |
| Sodium pyruvate | NA |
Additional information
| Size | 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 X 1 Gallon, 6 X 1 Gallon |
|---|
Documents
Certificate of Analysis (COA): Available upon request for your specific order — email info@purmabiologics.com and we will send the current lot documentation.
Safety Data Sheet (SDS): Available upon request — email info@purmabiologics.com.
What our customers say about PurMa Biologics
Feedback from labs we supply, across our full catalogue.
We have been working with PurMa biologics for the past several years, we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum. We appreciate the high quality and reasonable price, and great customer service.
We purchase everything we need related to cell and tissue culture from PurMa, reagents like: DMEM, LL15, EMEM, FBS, trypsin, antibiotics mix100X and... Always great quality, fast processing and shipping. We are totally satisfied.
Very fast, efficient, and economic service. Great for new PIs like me.
it is reliable vendor!
We always buy our agarose from PurMa and have never been disappointed.
I recently had the pleasure of interacting with Dr. Seif Azadi from Purma Biologics, and I cannot thank him enough for his expertise and prompt assistance. His deep knowledge and dedication were instrumental in meeting our laboratory’s needs with remarkable efficiency. We ordered FBS, DMEM, and Trypsin from Purma Biologics, and I’m thrilled to report that all the products have performed exceptionally well. They consistently deliver reproducible results, significantly enhancing the reliability of our experiments. Thank you, Team Purma Biologics, for your good products and support!
Frequently asked questions
What is the difference between the water grades?
Cell culture suited water is purified and tested for endotoxin and cell culture performance. Molecular biology grade water is targeted at nuclease removal. DEPC treated water is specifically treated to inactivate RNases. Choosing on price rather than grade is a common cause of unexplained assay failure.
What is the difference between DEPC treated and non-DEPC water?
DEPC treatment inactivates RNases and is used for RNA work. Non-DEPC nuclease free water reaches the same nuclease free standard without DEPC, which matters because residual DEPC can carboxymethylate RNA and interfere with some downstream enzymatic steps.
Is Ultra-Pure Cell Culture Suited Water endotoxin tested?
Yes. Each lot is tested and the result is reported on the Certificate of Analysis. Endotoxin matters even in water, because it survives autoclaving and carries straight through into your culture.
How should it be stored?
Store sealed at room temperature unless the label states otherwise, and avoid repeated opening. Once opened, the practical risk is airborne contamination rather than chemical change, so decant rather than pipetting repeatedly from the stock bottle.
Can I autoclave it myself instead?
Autoclaving sterilises but does not remove endotoxin, nucleases or ionic contaminants, and it will not turn general purpose water into cell culture grade. For culture and molecular work the starting purity is what matters.
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