- ✓Proudly made in the USA, manufactured in house
- ✓Certificate of Analysis available for every lot
- ✓Backed by decades of cell culture and protein biochemistry expertise
- ✓Bulk pricing, purchase orders, and custom formulations welcome
Ultra-Pure Nuclease Free Water (Non-DEPC Treated)
PurMa™ Ultra-Pure Nuclease Free Water (Non-DEPC Treated) is specialized for highly sophisticated molecular biology studies. Moreover, PurMa Biologics manufactures this water by double – Distillation as well as sterilizing by passing through 0.1µm filters.
Description
Background
PurMa™ Ultra-Pure Nuclease Free Water (Non-DEPC Treated) is specialized for highly sophisticated molecular biology studies. Moreover, this water possesses the highest standards for molecular biology studies by removing all contaminants such as:
- Organic and non-organic compounds.
- Dissolved and particulate matters with any sizes.
- Volatile and non-volatile
- Reactive, and inert
- Hydrophilic and hydrophobic
- Dissolved gases.
Special Features
- Purified by Deionization (DI) followed by Reverse Osmosis process (18.2 ΩM/cm2).
- Additionally, the trace of heavy metals including Ca+2 and Mg +2 has been depleted using Ion exchange chromatography resins.
- Moreover, it is Double – Distilled.
- Sterilized by 0.1 µm filters.
- And lastly, Packed under N2
Important Note: Do NOT expose this water to open air for a long time as it absorbs traces of various ions including bicarbonate.
PurMa™ Non DEPC Treated Ultra-Pure Nuclease Free Water is suitable for high accuracy molecular biology studies, as well as analytical analysis applications such as:
- It is definitely the best choice for mRNA & Total RNA Isolation.
- Mass spectrometry certainly performs the best with this quality of water.
- Electrophoresis, cloning, PCR, RNA, and DNA isolation.
- Especially, chromosome analysis or karyotyping as they need high quality water.
- Also, northern and southern blotting.
- Evaluated for proteases.
- Evaluated for phosphatases.
References
- A Review on Reverse Osmosis and Nanofiltration Membranes for Water Purification. Yang et al. Polymers (Basel). 2019 Jul 29;11(8):1252. doi: 10.3390/polym11081252. PMID:
- Removal of emerging organic micropollutants via modified-reverse osmosis/nanofiltration membranes: A review. Khoo et al. 2022 Oct;305:135151. 2022.135151. Epub 2022 May 30.
| General specifications | |
| Parameter | Specification |
| Grade | Ultra-Pure Molecular Biology |
| Appearance | Clear |
| pH | 5-7 |
| Osmolality | 275-360 mOsm/L |
| Filtration | Sterile filtered using 0.1 µm filter |
| Indicator | NA |
| Conductivity (Measured) | 0.056 µs/cm2 |
| Resistancy (Calculated) | 18.2 Ωm |
| Shipping Condition | Ambient |
| Refractive index | n20/D 1.34 (lit.) |
| Molecular Biology Parameters | |
| DNases & RNases | NOT Detected |
| proteases | NOT Detected |
| phosphatases | NOT Detected |
| Pathogens Evaluation | |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Mold & Fungi/Yeast | Negative |
| Bacteria | Negative |
| Sodium pyruvate | NA |
Additional information
| Size | 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 X 1 Gallon, 6 X 1 Gallon |
|---|
Documents
Certificate of Analysis (COA): Available upon request for your specific order — email info@purmabiologics.com and we will send the current lot documentation.
Safety Data Sheet (SDS): Available upon request — email info@purmabiologics.com.
What our customers say about PurMa Biologics
Feedback from labs we supply, across our full catalogue.
We always buy our agarose from PurMa and have never been disappointed.
I recently had the pleasure of interacting with Dr. Seif Azadi from Purma Biologics, and I cannot thank him enough for his expertise and prompt assistance. His deep knowledge and dedication were instrumental in meeting our laboratory’s needs with remarkable efficiency. We ordered FBS, DMEM, and Trypsin from Purma Biologics, and I’m thrilled to report that all the products have performed exceptionally well. They consistently deliver reproducible results, significantly enhancing the reliability of our experiments. Thank you, Team Purma Biologics, for your good products and support!
It’s been a pleasure working with PURMA Biologics. Their service is efficient, delivery is fast, and the quality of their products has met our expectations consistently. Very smooth collaboration overall.
PurMa products are of excellent quality and highly reliable.
I've ordered cell culture media multiple times. Great prices and excellent customer service. The ordering process was smooth, and everything arrived as expected. I'll definitely be purchasing from them again.
We buy all of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
Frequently asked questions
What is the difference between the water grades?
Cell culture suited water is purified and tested for endotoxin and cell culture performance. Molecular biology grade water is targeted at nuclease removal. DEPC treated water is specifically treated to inactivate RNases. Choosing on price rather than grade is a common cause of unexplained assay failure.
What is the difference between DEPC treated and non-DEPC water?
DEPC treatment inactivates RNases and is used for RNA work. Non-DEPC nuclease free water reaches the same nuclease free standard without DEPC, which matters because residual DEPC can carboxymethylate RNA and interfere with some downstream enzymatic steps.
Is Ultra-Pure Nuclease Free Water (Non-DEPC Treated) endotoxin tested?
Yes. Each lot is tested and the result is reported on the Certificate of Analysis. Endotoxin matters even in water, because it survives autoclaving and carries straight through into your culture.
How should it be stored?
Store sealed at room temperature unless the label states otherwise, and avoid repeated opening. Once opened, the practical risk is airborne contamination rather than chemical change, so decant rather than pipetting repeatedly from the stock bottle.
Can I autoclave it myself instead?
Autoclaving sterilises but does not remove endotoxin, nucleases or ionic contaminants, and it will not turn general purpose water into cell culture grade. For culture and molecular work the starting purity is what matters.
Related products
-
- Sale!
- Trypsin EDTA and EDTA-Free, Trypsin With EDTA
Trypsin-EDTA 0.25%
- Price range: $13.99 through $412.88
- Select options This product has multiple variants. The options may be chosen on the product page
-
- Sale!
- Cell Culture Suited Water and Water Treatments
General Molecular Biology Grade Water
- Price range: $21.29 through $598.23
- Select options This product has multiple variants. The options may be chosen on the product page
-
- Sale!
- Cell Culture Suited Water and Water Treatments
Ultra-Pure DEPC Treated Nuclease Free Water
- Price range: $32.99 through $900.56
- Select options This product has multiple variants. The options may be chosen on the product page
Reviews