PurMa™ Trypsin-EDTA contains ultra-pure trypsin. Furthermore, trypsin comes from one of the members of the serine protease family which goes through a set of intracellular signaling results in the activation of this enzyme in pancreas. Cell culture laboratories widely use trypsin to dissociate the adherent cells for routine cell culture. Trypsin performs the best at 37oC and at the PH 7.3-8.5.
Trypsin cleaves its substrates: IGDKLIPA . PurMa biologics purifies Trypsin using HPLC , 99% form bovine pancreas. Moreover, optical rotatory dispersion of trypsin in the presence of calcium shows a major conformational transition between 40 and 45° which results in deactivation of trypsin. Therefore, all the buffers needed to be Ca+2 & Mg+2 free for the proper function of this enzyme.
Trypsin treatment abolishes the ability of mycoplasmas to attach to the cells. Mycoplasma slowly contaminates and deteriorating cell culture, especially due to the invisibility of the contamination. PurMa Biologics manufactures all the reagents you need to fight mycoplasma in your cell culture. The presence of a trace of SDS and other detergents leads to a decrease in the trypsin activity.
sulfation of Tyr154 modulates the activity of this enzyme . To overcome the mentioned issue, we use our most sophisticated water quality to ensure the absence of Mg+2 and Ca+2 as well as other heavy metals, please see click here: PurMa™ Ultra-Pure Cell Culture Suited Water (Cat# P3W110102)
Additionally, we include 2mM EDTA as it Chelates metals such as calcium, magnesium, lead, mercury, copper, iron…
we manufactures 0.05%, 0.25%,0.5%, 2.5% and 5% Trypsin-EDTA as well as without EDTA in phenol red or without phenol red. Moreover, PurMa™ Trypsin is made in Hanks’ Buffer with 2mM EDTA (pH 7.8).
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- Sale!
- Trypsin With EDTA, Trypsin EDTA and EDTA-Free
Trypsin-EDTA 0.05%
- $16.19 – $764.07
- PurMa™ Trypsin-EDTA 0.05% contains ultra-pure trypsin. Furthermore, trypsin is one of the members of the serine protease family. Cell culture laboratories widely use trypsin to dissociate the adherent mammalian cells for routine cell culture. The Trypsin family is conservative. Trypsin performs the best at 37oC and at the PH 7.3-8.5.
- Select options
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- Sale!
- Trypsin With EDTA, Trypsin EDTA and EDTA-Free
Trypsin-EDTA 0.25%
- $14.88 – $818.07
- PurMa™ Trypsin-EDTA 0.25% contains ultra-pure trypsin. Furthermore, trypsin is one of the members of the serine protease family. Cell culture laboratories widely use trypsin to dissociate the adherent mammalian cells for routine cell culture. Trypsin performs the best at 37oC and at the PH 7.3-8.5.
- Select options
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- Sale!
- Trypsin With EDTA, Trypsin EDTA and EDTA-Free
Trypsin-EDTA 0.5%
- $16.19 – $801.71
- PurMa™ Trypsin-EDTA 0.5% contains ultra-pure trypsin. Furthermore, trypsin is one of the members of the serine protease family. Cell culture laboratories widely use trypsin to dissociate the adherent mammalian cells for routine cell culture. The Trypsin family is conservative. Trypsin performs the best at 37oC and at the PH 7.3-8.5.
- Select options
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- Sale!
- Trypsin With EDTA, Trypsin EDTA and EDTA-Free
Trypsin-EDTA 2.5%
- $38.15 – $818.07
- PurMa™ Trypsin-EDTA 2.5% contains ultra-pure trypsin. Furthermore, trypsin is one of the members of the serine protease family . Cell culture laboratories widely use trypsin to dissociate the adherent mammalian cells for routine cell culture. The Trypsin family is conservative. Trypsin performs the best at 37oC and at the PH 7.3-8.5.
- Select options
-
- Sale!
- Trypsin With EDTA, Trypsin EDTA and EDTA-Free
Trypsin-EDTA 5%
- $62.90 – $1615.03
- PurMa™ Trypsin-EDTA 5% contains ultra-pure trypsin. Furthermore, trypsin is one of the members of the serine protease family . Cell culture laboratories widely use trypsin to dissociate the adherent mammalian cells for routine cell culture. The Trypsin family is conservative. Trypsin performs the best at 37oC and at the PH 7.3-8.5.
- Select options
