Sale!
Trypsin - EDTA
  • ✓Proudly made in the USA, manufactured in house
  • ✓Certificate of Analysis available for every lot
  • ✓Backed by decades of cell culture and protein biochemistry expertise
  • ✓Bulk pricing, purchase orders, and custom formulations welcome
Request a quote or purchase order
Or call (866) 720-6116 · Mon to Fri, 10am to 5pm CT
SKU: N/A Categories: Trypsin EDTA and EDTA-Free, Trypsin With EDTA Tags: EDTA, Trypsin, Trypsin with EDTA 5%

Trypsin-EDTA 5%

Ultra-pure Trypsin-EDTA at 5% for routine dissociation of adherent mammalian cells.

  • Ultra-pure trypsin, a serine protease, for detaching adherent cells
  • Performs best at 37°C and pH 7.3 to 8.5
SKU: N/A Categories: Trypsin EDTA and EDTA-Free, Trypsin With EDTA Tags: EDTA, Trypsin, Trypsin with EDTA 5%

Description

Background

PurMa™ Trypsin-EDTA 5% contains ultra-pure trypsin. Furthermore, trypsin is one of the members of the serine protease family which goes through a set of intracellular signaling that results in the activation of this enzyme in the pancreas. Cell culture laboratories widely use trypsin to dissociate the adherent cells for routine cell culture. The Trypsin family is conservative. Trypsin performs the best at 37°C and at the PH 7.3-8.5.

Trypsin Activity

Trypsin cleaves its substrates: IGDKLIPA unless these amino acids are followed by proline. PurMa™ Trypsin is HPLC purified 99% purity for bovine pancreas. Moreover, optical rotatory dispersion of trypsin in the presence of calcium shows a major conformational transition between 40 and 45° which results in deactivation of trypsin. Therefore, all the buffers needed to be Ca+2 & Mg+2 free for the proper function of this enzyme.

PurMa™ Trypsin Deactivates Mycoplasma in your Cell Culture

Trypsin treatment abolishes the ability of mycoplasmas to attach to the cells. Mycoplasma is one of the common and slowly deteriorating contaminations in cell culture, especially due to the invisibility of the contamination. PurMa Biologics manufactures all the reagents you need to fight mycoplasma in your cell culture. The presence of a trace of SDS and other detergents leads to a decrease in the trypsin activity.

Preparation

The activity of this enzyme is further modulated by the sulfation of Tyr154. To overcome the mentioned issue, we use our most sophisticated water quality to ensure the absence of Mg+2 and Ca+2 as well as other heavy metals, please click here: PurMa™ Ultra-Pure Cell Culture Suited Water (Cat# P3W110102)

Additionally, we include 2mM EDTA as it chelates metals such as calcium, magnesium, lead, mercury, copper, iron…

Features and Benefits

  • PurMa™ Trypsin does not contain calcium or magnesium ions or any other heavy metals.
  • PurMa™ Trypsin does not contain serine protease inhibitors, including DFP, TLCK, APMSF, AEBSEF.
  • PurMa™ Trypsin does not contain Phytates or salts of phytic acid, such as myo-inositol which is commonly present in cell culture media and inhibits trypsin activity.
  • PurMa™ Trypsin has been rigorously evaluated for yeast, mold, mycoplasma, and endotoxin contamination.

Different Concentrations of Trypsin

PurMa Biologics manufactures 0.05%, 0.25%,0.5%, 2.5% and 5% Trypsin with EDTA as well as without EDTA in phenol red or without phenol red. Moreover, PurMa™ Trypsin is made in Hanks’ Buffer with 2mM EDTA (pH 7.8).

Formulation

The complete formulation can be found here: Trypsin with EDTA 5% Formulation

References

  1. Fate of trypsin and chymotrypsin in the human small intestine. Goldberg et al. 1969 Jun;10(6):477-83. doi: 10.1136/gut.10.6.477. PMID: 5786145.
  2. Adhesion of mycoplasmas to eukaryotic cells. Razin et al. Ciba Found Symp. 1981;80:98-118. doi: 10.1002/9780470720639.ch8. PMID: 6790254.
  3. Effects of hyaluronidase, trypsin, and EDTA on surface composition and topography during detachment of cells in culture. Vogel KG. Exp Cell Res. 1978 May;113(2):345-57. doi: 10.1016/0014-4827(78)90375-0. PMID: 122554.
Parameter Specification
Appearance Red, clear liquid
pH  7.8 ± 0.1
Osmolality  275-360 mOsm/L
Endotoxin  NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive  EDTA
Indicator  Phenol red
Mycoplasma Detection Negative
Sterility Tested  Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

 

Learn more: What Is Trypsin?

Additional information

Condition

Trypsin 5%, Standard Formulation, Trypsin 5%, Without Sodium Bicarbonate, Trypsin 5%, Without Calcium Chloride, Trypsin 5%, Without Glucose
Size

1 x 100 ml, 6 x 100 ml, 1 x 500 ml, 6 x 500 ml
Phenol Red

With Phenol Red, Without Phenol Red

Documents

Certificate of Analysis (COA): Available upon request for your specific order — email info@purmabiologics.com and we will send the current lot documentation.

Safety Data Sheet (SDS): Available upon request — email info@purmabiologics.com.

Reviews

There are no reviews yet.

Be the first to review “Trypsin-EDTA 5%”

Your email address will not be published. Required fields are marked *

Frequently asked questions

What sizes does Trypsin-EDTA 5% come in?

Trypsin-EDTA 5% is supplied in 1 x 100 mL, 6 x 100 mL, 1 x 500 mL and 6 x 500 mL, with or without phenol red.

Is 5% trypsin the right concentration for routine passaging?

For routine passaging of most adherent lines, 0.05% or 0.25% Trypsin-EDTA is the usual choice. 5% is a concentrated formulation intended for dilution or for particularly stubborn tissue and primary preparations. If you are passaging a standard cell line, the 0.05% or 0.25% products are likely the better fit.

Can I get it without calcium chloride or sodium bicarbonate?

Yes. Alongside the standard formulation, Trypsin 5% is available without sodium bicarbonate, without calcium chloride and without glucose.

Is it sterile, and do you provide a Certificate of Analysis?

Yes. Every lot is manufactured and quality controlled in house in Oklahoma City, and a Certificate of Analysis is available for every lot. Storage conditions and the expiry date for your specific lot are printed on the bottle and stated on that lot's COA.

Can you supply trypsin in bulk or on a purchase order?

Yes. Custom formulations, non standard fill volumes and bulk orders are all available, since everything is made in house. Purchase orders are accepted and bulk pricing is available. Request a quote from this page and we will come back with pricing and lead time.

Related products