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Water Bath Treatment (500X)
At 37°C, water creates humidity which makes an excellent environment for growing all types of pathogens. Basically, growing various pathogens in a water bath container in the absence of a prevention agent is inevitable. PurMaTM Water Bath Treatment (500X) prevents growing all the known pathogens in water bath incubators.
Description
Background
At 37°C, water creates humidity which makes an excellent environment for growing all types of pathogens. Basically, growing various pathogens in a water bath container in the absence of a preventing agent is inevitable. PurMaTM Water Bath Treatment (500X) prevents growing all the known pathogens in water bath incubators.
Special Features
- Firstly, dilute PurMaTM Water Bath Treatment 500 times.
- Secondly, PurMa™ Water Bath contains fast-acting biocidal agents with a moderately long duration of action.
- PurMaTM Water Bath Treatment certainly does not cause damage to the stainless steel or copper tray.
- Moreover, it is non-toxic, non-volatile.
- It actively eliminates bacteria, mold, yeast and most viruses, fungi, and protozoa.
Important Notes
- If the below instruction is followed, PurMa Biologics guarantees NO pathogen growth in your water bath for the duration of 8 weeks.
- If you, however, go above 500 times dilution, the treatment will be too weak to be effective.
- PurMa™ General Water Bath treatment is not intended for a CO2 incubator.
Instruction to Use
- Primarily, Spray water bath with 70% alcohol before using this product.
- Dilute the PurMaTM Water Bath Treatment 500 times with either molecular biology or cell culture suited water.
- Importantly, avoid leaving the water bath lid open overnight, add water if needed.
References:
- Effects of CO2 water-bath treatment on blood flow and angiogenesis in ischemic hind limb of diabetic rat. Xu et al. Can J Physiol Pharmacol. 2018 Oct;96(10):1017-1021.. Epub 2018 May 30.
- Water-bath method for sonographic evaluation of superficial structures of the extremities in children. Krishnamurthy et al. Pediatr Radiol. 2013 Mar;43 Suppl 1:S41-7. Epub 2013 Mar 12.
| Parameter | Specification |
| Appearance | Red liquid |
| pH | 7.4 ± 0.1 |
| Osmolality | 275-360 mOsm/L |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Suitability | Suitable for mammalian cell culture |
| Indicator | NA |
| Sterility Tested | Sterile filtered using 0.22 µm filter |
| Form | Liquid |
| Shipping Condition | Dry Ice |
Additional information
| Size | 1 x 100 ml, 1 x 500 ml |
|---|
Documents
Certificate of Analysis (COA): Available upon request for your specific order — email info@purmabiologics.com and we will send the current lot documentation.
Safety Data Sheet (SDS): Available upon request — email info@purmabiologics.com.
What our customers say about PurMa Biologics
Feedback from labs we supply, across our full catalogue.
PurMa products are of excellent quality and highly reliable.
I've ordered cell culture media multiple times. Great prices and excellent customer service. The ordering process was smooth, and everything arrived as expected. I'll definitely be purchasing from them again.
We buy all of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
We have been working with PurMa biologics for the past several years, we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum. We appreciate the high quality and reasonable price, and great customer service.
We purchase everything we need related to cell and tissue culture from PurMa, reagents like: DMEM, LL15, EMEM, FBS, trypsin, antibiotics mix100X and... Always great quality, fast processing and shipping. We are totally satisfied.
Very fast, efficient, and economic service. Great for new PIs like me.
Frequently asked questions
What is the difference between the water grades?
Cell culture suited water is purified and tested for endotoxin and cell culture performance. Molecular biology grade water is targeted at nuclease removal. DEPC treated water is specifically treated to inactivate RNases. Choosing on price rather than grade is a common cause of unexplained assay failure.
What is the difference between DEPC treated and non-DEPC water?
DEPC treatment inactivates RNases and is used for RNA work. Non-DEPC nuclease free water reaches the same nuclease free standard without DEPC, which matters because residual DEPC can carboxymethylate RNA and interfere with some downstream enzymatic steps.
Is Water Bath Treatment (500X) endotoxin tested?
Yes. Each lot is tested and the result is reported on the Certificate of Analysis. Endotoxin matters even in water, because it survives autoclaving and carries straight through into your culture.
How should it be stored?
Store sealed at room temperature unless the label states otherwise, and avoid repeated opening. Once opened, the practical risk is airborne contamination rather than chemical change, so decant rather than pipetting repeatedly from the stock bottle.
Can I autoclave it myself instead?
Autoclaving sterilises but does not remove endotoxin, nucleases or ionic contaminants, and it will not turn general purpose water into cell culture grade. For culture and molecular work the starting purity is what matters.
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