Chemically Defined McCoy’s 5A Modified Media (CD-M5AMM)
Chemically Defined McCoy’s 5A Modified Media (CD-M5AMM) does not need any serum, fully synthetic and if free of any trace of animal products. Additionally, the cell growth with CD- McCoy’s 5A is equivalent to Medium+ 10% FBS.
- ✓Proudly made in the USA, manufactured in house
- ✓Certificate of Analysis available for every lot
- ✓Backed by decades of cell culture and protein biochemistry expertise
- ✓Bulk pricing, purchase orders, and custom formulations welcome
Description
Background
PurMa™ Chemically Defined McCoy’s 5A Modified Media (CD-M5AMM) is a proprietary formulation used to grow cells in vitro without any undefined or animal-derived components. Moreover, (CD-M5AMM)contains known quantities of pure chemicals, including amino acids, vitamins, salts, sugars, and more importantly a proprietary combination of synthetic growth factors.
It is absolutely crucial to mention that the PurMa™ Growth Supplement for each conventional media such as DMEM, IMDM, RPMI-1640… (base media ) are slightly different in combination, amount of several growth factors, ions, chemicals, pH with particular adjustments needs to be done, based on the environments the base media provides.
This provides a consistent and controlled environment for cell growth and research. Noticeably, (CD-M5AMM) is essential for applications that require reproducibility and regulatory compliance as well as for studying specific cellular processes, without extraneous variables. Furthermore, (CD-M5AMM) is designed for custom formulations tailored for various cells.
Important Notes:
It is crucially important to have a gradual transition from media containing FBS to chemically defined cell culture media. Follow these steps:
- Starting Point: 10% FBS+ (control) (you need two controls, one to be left in only 10% FBS and continue monitoring for comparing with 0% FBS + PurMa™ Chemically Defined Medium. The second one is chemically defined medium + 10% FBS, which is going through the reduction of FBS process
- 48 hours later: 8% FBS in + PurMa™ Chemically Defined Medium
- 48 hours later: 6% FBS in + PurMa™ Chemically Defined Medium
- 48 hours later: 4% FBS + PurMa™ Chemically Defined Medium
- 48 hours later: 2% FBS + PurMa™ Chemically Defined Medium
- 48 hours later: 1% FBS + PurMa™ Chemically Defined Medium
- 24 hours later: 0% FBS and only PurMa™ Chemically Defined Medium
Importantly, for each change, spin down and fully discard the media and replace it with the next lower percentage of FBS.
Noticeably, you don’t have to start with a huge number of cells (~500,000 cells/ml). Additionally, we strongly recommend you start with more than one plate as some of the plates might get contaminated
So, once the cells are kept in chemically defined Media, you should always keep them in the same chemically defined medium from the same vendor and the same formulation, even for freezing (see below).
Usually, after you get your cells adapted to PurMa™ Chemically Defined Medium you need to make several plates and freeze down several vials. The mentioned cells are adapted to chemically defined medium and when you thaw them in the first week, in addition to PurMa™ Chemically Defined Medium, you need to add 10% PurMa™ CD Booster (Cat# P3C10144).
Formulation
The (CD-M5AMM) includes a base formulation including L-Alanine -L glutamine :Base Formulation of CD McCoy’s 5A P3C310724
Additionally, (CD-M5AMM) contains a proprietary synthetic cocktail of specific growth factors, hormones, and other nutrients which makes it independent of any serum and any trace of animal products.
References
- Altered polyamine metabolism in Chinese hamster cells growing in a defined medium. Sertich GJ, et al. J Cell Physiol. 1986 Apr;127(1):114-20. doi: 10.1002/jcp.1041270115.PMID: 3958058
- Investigations on in vitro survival and virulence of T. pallidum under aerobiosis. Rathlev T. Br J Vener Dis. 1975 Oct;51(5):296-300. doi: 10.1136/sti.51.5.296. PMID: 172190
| Parameter | Specification |
|---|---|
| Appearance | Red, clear liquid |
| pH | 7.2 ± 0.1 |
| Osmolality | 293-355 mOsm/L |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Suitability | Suitable for mammalian cell culture |
| Additive | Sodium pyruvate |
| Indicator | Phenol red |
| Mycoplasma Detection | Negative |
| Sterility Tested | Sterile filtered using 0.0.1 µm filter. 3 Times |
| Form | Liquid |
| Shipping Condition | Room temperature, Over night |
Learn more: What Is McCoy’s 5A?
Additional information
| Condition | CD- M5AMM- Standard Formulation, CD- M5AMM Without o L-Alanyl-L-Glutamine, CD- M5AMM -Without Phenol Red, CD- M5AMM -Without Sodium Bicarbonate, CD- M5AMM – Without Glucose, CD- M5AMM – Without Peptone, CD- M5AMM – w/o o L-Alanyl-L-Glutamine, w/o Phenol Red, CD- M5AMM – Without Phenol Red, Without Sodium Bicarbonate, CD- M5AMM – w/ o L-Alanyl-L-Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, CD- M5AMM – Low Glucose (1.5 g/L), CD- M5AMM – Low Sodium Bicarbonate (0.35 g/L), CD- M5AMM – Low Sodium Bicarbonate (0.85 g/L), CD- M5AMM -15 mM HEPES (3.6 g/L), CD- M5AMM – 25 mM HEPES (5.9 g/L) |
|---|---|
| Size | 1X 100 ml, 1X 1000 ml, 1X 500 ml |
Documents
Certificate of Analysis (COA): Available upon request for your specific order — email info@purmabiologics.com and we will send the current lot documentation.
Safety Data Sheet (SDS): Available upon request — email info@purmabiologics.com.
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