Chemically Defined McCoy 5A medium
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SKU: Select options below to see it Categories: Cell Culture Media, McCoy’s 5A Modified Media (M5AMM) Tags: Advanced, Cell Culture, Human Transferrin, L-Alanyl-L-Glutamine, M5AMM, McCoy's 5A, Recombinant Insulin, Serum Reduction

Chemically Defined McCoy’s 5A Modified Media (CD-M5AMM)

(1 customer review)

Price on request

Chemically Defined McCoy’s 5A Modified Media (CD-M5AMM)  does not need any serum, fully synthetic and if free of any trace of animal products. Additionally, the cell growth with CD- McCoy’s 5A  is equivalent to Medium+ 10% FBS.

This product is priced per order. Choose the options you need, then request a quote and we will reply by email.

SKU Select options below to see it

For research use only. Not for human, veterinary or diagnostic use.

Description

Background

PurMa™ Chemically Defined McCoy’s 5A Modified Media (CD-M5AMM) is a proprietary formulation used to grow cells in vitro without any undefined or animal-derived components. Moreover, (CD-M5AMM)contains known quantities of pure chemicals, including amino acids, vitamins, salts, sugars, and more importantly a proprietary combination of synthetic growth factors.

It is absolutely crucial to mention that the PurMa™ Growth Supplement for each conventional media such as DMEM, IMDM, RPMI-1640… (base media ) are slightly different in combination, amount of several growth factors, ions, chemicals,  pH with particular adjustments needs to be done, based on the environments the base media provides.

This provides a consistent and controlled environment for cell growth and research. Noticeably, (CD-M5AMM) is essential for applications that require reproducibility and regulatory compliance as well as for studying specific cellular processes, without extraneous variables. Furthermore, (CD-M5AMM) is designed for custom formulations tailored for various cells.

Chemically Defined McCoy Graph

Important Notes:

It is crucially important to have a gradual transition from media containing FBS to chemically defined cell culture media. Follow these steps:

  • Starting Point: 10% FBS+ (control) (you need two controls, one to be left in only 10% FBS and continue monitoring for comparing with 0% FBS + PurMa™ Chemically Defined Medium. The second one is chemically defined medium + 10% FBS, which is going through the reduction of FBS process
  • 48 hours later: 8% FBS in + PurMa™ Chemically Defined Medium
  • 48 hours later: 6% FBS in + PurMa™ Chemically Defined Medium
  • 48 hours later: 4% FBS + PurMa™ Chemically Defined Medium
  • 48 hours later: 2% FBS + PurMa™ Chemically Defined Medium
  • 48 hours later: 1% FBS + PurMa™ Chemically Defined Medium
  • 24 hours later: 0% FBS and only PurMa™ Chemically Defined Medium

Importantly, for each change, spin down and fully discard the media and replace it with the next lower percentage of FBS.

Noticeably, you don’t have to start with a huge number of cells (~500,000 cells/ml). Additionally, we strongly recommend you start with more than one plate as some of the plates might get contaminated

So, once the cells are kept in chemically defined Media, you should always keep them in the same chemically defined medium from the same vendor and the same formulation, even for freezing (see below).

Usually, after you get your cells adapted to PurMa™ Chemically Defined Medium you need to make several plates and freeze down several vials. The mentioned cells are adapted to chemically defined medium and when you thaw them in the first week, in addition to PurMa™ Chemically Defined Medium, you need to add 10% PurMa™ CD Booster (Cat# P3C10144).

Formulation

The (CD-M5AMM) includes a base formulation including L-Alanine -L glutamine :Base Formulation of CD McCoy’s 5A P3C310724

Additionally, (CD-M5AMM) contains a proprietary synthetic cocktail of specific growth factors, hormones, and other nutrients which makes it independent of any serum and any trace of animal products.

References

  1. Altered polyamine metabolism in Chinese hamster cells growing in a defined medium. Sertich GJ, et al. J Cell Physiol. 1986 Apr;127(1):114-20. doi: 10.1002/jcp.1041270115.PMID: 3958058
  2. Investigations on in vitro survival and virulence of T. pallidum under aerobiosis. Rathlev T. Br J Vener Dis. 1975 Oct;51(5):296-300. doi: 10.1136/sti.51.5.296. PMID: 172190
Parameter Specification
Appearance Red, clear liquid
pH  7.2 ± 0.1
Osmolality  293-355 mOsm/L
Endotoxin  NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive  Sodium pyruvate
Indicator  Phenol red
Mycoplasma Detection Negative
Sterility Tested  Sterile filtered using 0.0.1 µm filter. 3 Times
Form Liquid
Shipping Condition  Room temperature, Over night

Learn more: What Is McCoy’s 5A?

Additional information

Condition

CD- M5AMM- Standard Formulation, CD- M5AMM Without o L-Alanyl-L-Glutamine, CD- M5AMM -Without Phenol Red, CD- M5AMM -Without Sodium Bicarbonate, CD- M5AMM – Without Glucose, CD- M5AMM – Without Peptone, CD- M5AMM – w/o o L-Alanyl-L-Glutamine, w/o Phenol Red, CD- M5AMM – Without Phenol Red, Without Sodium Bicarbonate, CD- M5AMM – w/ o L-Alanyl-L-Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, CD- M5AMM – Low Glucose (1.5 g/L), CD- M5AMM – Low Sodium Bicarbonate (0.35 g/L), CD- M5AMM – Low Sodium Bicarbonate (0.85 g/L), CD- M5AMM -15 mM HEPES (3.6 g/L), CD- M5AMM – 25 mM HEPES (5.9 g/L)
Size

1X 100 ml, 1X 1000 ml, 1X 500 ml

Documents

Certificate of Analysis (COA): Available upon request for your specific order — email info@purmabiologics.com and we will send the current lot documentation.

Safety Data Sheet (SDS): Available upon request — email info@purmabiologics.com.

What our customers say about PurMa Biologics

Feedback from labs we supply, across our full catalogue.

★★★★★
We always buy our agarose from PurMa and have never been disappointed.
Zhenghui Liu Applied StemCell
★★★★★
I recently had the pleasure of interacting with Dr. Seif Azadi from Purma Biologics, and I cannot thank him enough for his expertise and prompt assistance. His deep knowledge and dedication were instrumental in meeting our laboratory’s needs with remarkable efficiency. We ordered FBS, DMEM, and Trypsin from Purma Biologics, and I’m thrilled to report that all the products have performed exceptionally well. They consistently deliver reproducible results, significantly enhancing the reliability of our experiments. Thank you, Team Purma Biologics, for your good products and support!
Ravi Gor University of Oklahoma
★★★★★
It’s been a pleasure working with PURMA Biologics. Their service is efficient, delivery is fast, and the quality of their products has met our expectations consistently. Very smooth collaboration overall.
Maya Hart Tak Biomed
★★★★★
PurMa products are of excellent quality and highly reliable.
East Tennessee State University Verified customer
★★★★★
I've ordered cell culture media multiple times. Great prices and excellent customer service. The ordering process was smooth, and everything arrived as expected. I'll definitely be purchasing from them again.
Ghazal Rastegar UT Dallas
★★★★★
We buy all of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
Gregory Mullen OU Health Sciences Center

1 review for Chemically Defined McCoy’s 5A Modified Media (CD-M5AMM)

  1. Verified Customer, University of Oklahoma –

  2. Verified Customer, University of Oklahoma –

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Frequently asked questions

How should I store Chemically Defined McCoy’s 5A Modified Media (CD-M5AMM)?

Store at 2 to 8 degrees C, protected from light, and do not freeze unless the label states otherwise. Freezing can precipitate salts and degrade light sensitive components such as riboflavin and folic acid. Always follow the storage line on the Certificate of Analysis for the specific lot.

What is the shelf life once opened?

Unopened and correctly stored, the typical shelf life is 12 months from manufacture. Once opened, use within about four weeks and always handle in a laminar flow hood, since the practical limit after opening is contamination risk rather than chemical stability.

Is it sterility and endotoxin tested?

Yes. Every lot is sterile filtered and tested for sterility and endotoxin, and a Certificate of Analysis is issued for each lot. The COA is available on request for any catalogue number.

Do I need to add L-glutamine?

It depends on the formulation you order. Standard formulations may contain free L-glutamine, which degrades in solution over time, while MAX formulations use a stable L-alanyl-L-glutamine dipeptide that does not require fresh supplementation. Check the option you have selected before adding more.

Can I order this in bulk or as a custom formulation?

Yes. Bulk volumes and custom formulations are made to order, including adjusted glucose, bicarbonate, buffering and cation content. Use the request bulk pricing button on this page and include the specification you need.

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