Description

PurMaTM Advanced McCoy’s 5A contains components that allow for a 60–90% reduction in FBS supplementation. Using synthetic components such as insulin recombinant full chain, recombinant albumin, and transferrin increase consistency in results with less variation amongst lot-to-lot serum changes.  In Advanced McCoy’s 5A, L‑alanine‑L‑glutamine is used as the source of glutamine.  Additionally, reducing FBS requirements lowers the cost of cell culture experiments. Advanced McCoy’s 5A  contains:

  • All the twenty essential and non-essential amino acids with higher concentrations than regular and MAX formulations.
  • It includes the elements which significantly reduce the need for a serum for cell culture including:

Advanced McCoy’s 5A  is a substantial upgrade to the standard and MAX for McCoy’s 5A. That is because media this media contains ingredients to allow for serum reduction, specifically: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, lipid rich PurMaTM Albumin, trace elements such as sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride.

  1. PurMaTM Albumin:  Chromatographically purified with IgG content <=0.1.0%.
  1. PurMaTM Human Transferrin (Holo): prevents Improperly iron storage and delivery in cell culture systems which is a major contributor to oxidative stress and protein damage.
  2. PurMaTM Insulin Recombinant
  3. Trace of other elements which mimic the elements in fetal bovine serum.

In another word, Advanced form of McCoy’s 5A is a significant step toward chemically defined form of this media where all elements are synthetic and the need for FBS is eliminated.

Chemically Defined McCoy’s 5A (CD- McCoy’s 5A) is the next generation of PurMa Biologics’ dedicated team of top scientists which is the outcome of decades of cutting-edge research. CD- McCoy’s 5A soon will be offered globally to laboratories who are looking for consistency and repeatability among their data.

Formulation:  Complete formulation is available here: Advanced McCoy Formulation

References:

  1. The survival of human skin stored by refrigeration at 4 degrees C in McCoy’s 5A medium: does oxygenation of the medium improve storage time? DeBono R, Rao GS, Berry RB.Plast Reconstr Surg. 1998 Jul;102(1):78-83. doi: 10.1097/00006534-199807000-00012.PMID: 9655410
  2. Mycoplasmal infection of insect cell cultures. Steiner T, McGarrity G.In Vitro. 1983 Sep;19(9):672-82. doi: 10.1007/BF02628958.PMID: 6413389
  3. Fresh and vitrified bovine preantral follicles have different nutritional requirements during in vitro culture. Castro SV, Carvalho AA, Silva CM, Santos FW, Campello CC, Figueiredo JR, Rodrigues AP.Cell Tissue Bank. 2014 Dec;15(4):591-601. doi: 10.1007/s10561-014-9432-2. Epub 2014 Mar 8.PMID: 24610241
  4. In vitro culture of postimplantation hamster embryos. Ebron-McCoy MT, Beyer PE, Oglesby LA, Kavlock RJ.Reprod Toxicol. 1988;2(1):31-6. doi: 10.1016/s0890-6238(88)80006-6.PMID: 2980399
  5. The turkey myogenic satellite cell: optimization of in vitro proliferation and differentiation. McFarland DC, Doumit ME, Minshall RD.Tissue Cell. 1988;20(6):899-908. doi: 10.1016/0040-8166(88)90031-6.PMID: 3245037
  6. Investigations on in vitro survival and virulence of T. pallidum under aerobiosis. Rathlev T. Br J Vener Dis. 1975 Oct;51(5):296-300. doi: 10.1136/sti.51.5.296. PMID: 172190
  7. Altered polyamine metabolism in Chinese hamster cells growing in a defined medium. Sertich GJ, Glass JR, Fuller DJ, Gerner EW.J Cell Physiol. 1986 Apr;127(1):114-20. doi: 10.1002/jcp.1041270115.PMID: 3958058
Parameter Specification
Appearance Red, clear liquid
pH  7.2 ± 0.1
Osmolality  275-360 mOsm/L
Endotoxin  NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive  Sodium pyruvate
Indicator  Phenol red
Mycoplasma Detection Negative
Sterility Tested  Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature