Advanced McCoy’s 5A Modified Media (A-M5AMM)

Advanced McCoy’s 5A Modified Media (A-M5AMM) contains L-Alanyl-L-Glutamine as well as ingredients to allow for serum reduction.  Additionally, Advanced McCoy’s 5A needs 2-5% FBS and provides more consistency.

Description

Advanced McCoy’s 5A Modified Media (A-M5AMM)

Background

PurMa Advanced McCoy’s 5A Modified Media (A-M5AMM) contains components that allow for a 60–90% reduction in FBS supplementation. More importantly, using synthetic components such as recombinant insulin, albumin, and transferrin increase consistency. This results in less variation amongst lot-to-lot serum changes. In Advanced McCoy’s 5A, L-Alanyl-L-Glutamine is used as the source of glutamine. Additionally, reducing FBS requirements lowers the cost of cell culture experiments.

Contents

Advanced McCoy’s 5A Contains:

  • includes all twenty essential and non-essential amino acids with higher concentrations than regular and MAX formulations.
  • Advanced McCoy’s 5A incorporates elements that significantly reduce the need for a serum for cell culture.

Crucial Ingredients:

  • PurMa™ Human Transferrin (Holo): prevents Improper iron storage and delivery in cell culture systems which is a major contributor to oxidative stress and protein damage.
  • PurMaTM Insulin Recombinant
  • Trace of other elements which mimic the elements in fetal bovine serum.

Consequently, Advanced McCoy’s 5A Modified Media (A-M5AMM) is a significant step toward a chemically defined form of this media. Unquestionably, Chemically Defined Advanced McCoy’s 5A (CD-A-M5AMM) is the outcome of decades of cutting-edge research and will soon be offered globally to laboratories that are looking for consistency and repeatability in their data.

Formulation

Complete formulation is available here: Advanced McCoy’s 5A Formulation

References

  1. Mycoplasmal infection of insect cell cultures. Steiner T, McGarrity G. In Vitro. 1983 Sep;19(9):672-82. doi: 10.1007/BF02628958.PMID: 6413389
  2. Investigations on in vitro survival and virulence of T. pallidum under aerobiosis. Rathlev T. Br J Vener Dis. 1975 Oct;51(5):296-300. doi: 10.1136/sti.51.5.296. PMID: 172190
  3. Altered polyamine metabolism in Chinese hamster cells growing in a defined medium. Sertich GJ, et al. J Cell Physiol. 1986 Apr;127(1):114-20. doi: 10.1002/jcp.1041270115.PMID: 3958058
Parameter Specification
Appearance Red, clear liquid
pH  7.2 ± 0.1
Osmolality  275-360 mOsm/L
Endotoxin  NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive  Sodium pyruvate
Indicator  Phenol red
Mycoplasma Detection Negative
Sterility Tested  Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

 

Additional information

Condition

A-M5AMM- Standard Formulation, A-M5AMM -Without L-Alanine-L-Glutamine, A-M5AMM -Without Phenol Red, A-M5AMM -Without Sodium Bicarbonate, A-M5AMM – Without Glucose, A-M5AMM – Without Peptone, A-M5AMM – w/o Glutamine, w/o Phenol Red, A-M5AMM – Without Phenol Red, Without Sodium Bicarbonate, A-M5AMM – w/o Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, A-M5AMM – Low Glucose (1.5 g/L), A-M5AMM – Low Sodium Bicarbonate (0.35 g/L), A-M5AMM – Low Sodium Bicarbonate (0.85 g/L), A-M5AMM -15 mM HEPES (3.6 g/L), A-M5AMM – 25 mM HEPES (5.9 g/L), A-M5AMM – 15 mM HEPES (3.6 g/L) , Low Sodium Bicarbonate (0.35 g/L), A-M5AMM – 15 mM HEPES (3.6 g/L) , Low Sodium Bicarbonate (0.85g/L), A-M5AMM – 25 mM HEPES (3.6 g/L) , Low Sodium Bicarbonate (0.35 g/L), A-M5AMM – 25 mM HEPES (5.9 g/L) , Low Sodium Bicarbonate (0.85 g/L)

Format

Liquid, Powder

Size

10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed

Quality Control

[vc-quality-control-tab]

Formulation

Advanced McCoy’s 5A Formulation

SDS

SDS

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