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William’s E Medium
Williams′ Medium E has been used for primary and also commercial cell lines including: primary liver epithelial cells, HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes ,carcinomas as well as hybridoma cells.
Description
Background
William’s E Medium is a modified version of Williams′ Medium D by Williams and Gunn. Although it is like MEM, there is a difference in glucose as well as amino acid contents. This media was originally used to isolate and culture primary liver epithelial cells, distinguishing epithelial-like cells from fibroblast-like cells in culture. It has been shown that this medium is suited for longer term adult primary cell culture.
PurMa™ William’s E Medium is
- Suited for long term culture.
- Enriched in amino acids.
- Double the glucose compared to the original formulation.
- Moreover, contains unique ingredients, including zinc, iron, manganese, non-essential amino acids, the reducing agent glutathione as well as lipid methyl linoleate
Moreover, PurMa™ William’s E Medium also contains:
- Low level of glucose (2.00 g/ liter). PurMa Biologics manufactures the high glucose (4.5 g/L) version of this media as well (Cat #: P3S029880)
- The standard formulation contains 2.2 g/L Sodium bicarbonate, but PurMa Biologics also manufactures the high sodium bicarbonate (3.7 g/L) Williams′ Medium E (Cat #: P3S032880)
- 2 mM L-glutamine (292 mg/L)
PurMa Biologics manufactures 112 types of William’s E Medium which is certainly the most comprehensive catalog of media.
Why PurMa Biologics? Why we stand behind our cell culture media:
Our products are the outcome of 30 years of experience in cutting edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. We utilize 99% pure amino acids. This will significantly increase the consistency among your experiments, and the result of your lab. Considering the highest quality, our prices are extremely reasonable.
Application of William’s E Medium
This media has elements supporting long term culturing of primary cells. It utilizes a bicarbonate buffering system. This media has been used for primary as well as commercial cell lines including: primary liver epithelial cells, HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, carcinomas, as well as hybridoma cells.
Formulation:
The complete formulation is available in formulation tab as well here:William’s E Medium Formulation
References
- Isolation and long-term cell culture of epithelial-like cells from rat liver. Williams GM, Weisburger et al. Exp Cell Res. 1971 Nov;69(1):106-12. doi: 10.1016/0014-4827(71)90316-8.
- Growth and continuous passage of COMMA-D mouse mammary epithelial cells in hormonally defined serum-free medium. Riss et al. Cancer Res. 1987 Jul 15;47(14):3776-82. PMID: 3297308.
| Parameter | Specification |
|---|---|
| Appearance | Red, clear liquid |
| pH | 7.2 ± 0.1 |
| Osmolality | 275-360 mOsm/L |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Suitability | Suitable for mammalian cell culture |
| Additive | Sodium pyruvate |
| Indicator | Phenol red |
| Mycoplasma Detection | Negative |
| Sterility Tested | Sterile filtered using 0.22 µm filter |
| Form | Liquid |
| Shipping Condition | Room temperature |
Learn more: What Is William’s E Medium?
Additional information
| Condition | William's E Standard Formulation, William's E w/o Glutamine, William's E w/o Phenol Red, William's E w/o Sodium Bicarbonate, William's E w/o Sodium Pyruvate, William's E w/o Glucose, William's E w/o Glutamine, w/o Phenol Red, William's E w/o Phenol Red, w/o Sodium Bicarbonate, William's E w/o Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, William's E High Glucose (4.5 g/L), William's E Low Sodium Bicarbonate (1.1 g/L), William's E 15 mM HEPES (3.6 g/L), William's E 25 mM HEPES (5.9 g/L), William's E 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.1 g/L), William's E 25 mM HEPES (5.9 g/L), Low Sodium Bicarbonate (1.1 g/L) |
|---|---|
| Format | Liquid, Powder |
| Size | 10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L |
Documents
Certificate of Analysis (COA): Available upon request for your specific order — email info@purmabiologics.com and we will send the current lot documentation.
Safety Data Sheet (SDS): Available upon request — email info@purmabiologics.com.
What our customers say about PurMa Biologics
Feedback from labs we supply, across our full catalogue.
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It’s been a pleasure working with PURMA Biologics. Their service is efficient, delivery is fast, and the quality of their products has met our expectations consistently. Very smooth collaboration overall.
PurMa products are of excellent quality and highly reliable.
I've ordered cell culture media multiple times. Great prices and excellent customer service. The ordering process was smooth, and everything arrived as expected. I'll definitely be purchasing from them again.
We buy all of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
Frequently asked questions
What sizes does William's E Medium come in?
William's E Medium is supplied in 500 mL and 1 L bottles, in single bottles or cases of 6 or 10, plus 10 L and 50 L bulk volumes. Both liquid and powder formats are available.
Can I get William's E Medium without glutamine or phenol red?
Yes. Alongside the standard formulation we make it without L-glutamine, without phenol red, without sodium bicarbonate, without sodium pyruvate and without glucose. It is available as liquid or as powder.
Is William's E Medium suitable for primary hepatocyte culture?
Yes. William's E was developed for the long term culture of adult liver epithelial cells and remains the standard basal medium for primary hepatocytes, as well as other specialised cultures that need a nutrient rich base.
Is William's E Medium sterile, and do you provide a Certificate of Analysis?
Yes. Every lot is manufactured and quality controlled in house in Oklahoma City, and a Certificate of Analysis is available for every lot. Storage conditions and the expiry date for your specific lot are printed on the bottle and stated on that lot's COA.
Can you make a custom William's E Medium formulation or supply it in bulk?
Yes. Because PurMa manufactures in house rather than reselling, any formulation on this page can be adjusted to your specification and filled in custom volumes. Bulk pricing and purchase orders are welcome, and academic and institutional POs are standard. Use the quote button on this page and the product name is carried across for you.
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