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Williams′ Medium E
Background
Williams′ Medium E is a modification version of Williams′ Medium D by Williams and Gunn. Although, it is like MEM, but there is difference in glucose as well as amino acid contents. This media was originally used to isolate and culture primary liver epithelial cells from Epithelial-like cells of fibroblast-like cells culture. It has been shown that this medium is suited for longer term adult primary cell culture.
PurMa™ William's E Medium is
- Suited for long term culture.
- Enriched in amino acids.
- Double the glucose compared to the original formulation.
- Moreover, contains unique ingredients, including zinc, iron, manganese, non-essential amino acids, the reducing agent glutathione as well as lipid methyl linoleate
Moreover, PurMa™ William's E Medium also contains:
- Low level of glucose (2.00 g/ liter). PurMa Biologics manufacturers the high glucose (4.5 g/L) version of this media as well (Cat #: P3S029880)
- Although the standard formulation contains 2.2 g/L Sodium bicarbonate, but PurMa Biologics manufacturers the high sodium bicarbonate (3.7 g/L) Williams′ Medium E as well (Cat #: P3S032880)
- 2 mM L-glutamine (292 mg/L)’
PurMa Biologics manufactures 112 types of William's E Medium which is certainly the most comprehensive catalog of media.
Why PurMa Biologics? Why we stand behind our cell culture media:
Our products are the outcome of 30 years of experience in cutting edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. We utilize %99 pure amino acids. This will significantly increase the consistency among your experiments, and the result of your lab. Considering the highest quality, our prices are extremely reasonable.
Application of William's E Medium
This media has elements supporting long term culturing of primary cells.. It utilizes a bicarbonate buffering system. This media has been used for primary as well as commercial cell lines including: primary liver epithelial cells, HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes ,carcinomas as well as hybridoma cells.
Formulation:
The complete formulation is available in formulation tab as well here:
William's E Medium Formulation
References
- Isolation and long-term cell culture of epithelial-like cells from rat liver. Williams GM, Weisburger et al. Exp Cell Res. 1971 Nov;69(1):106-12. doi: 10.1016/0014-4827(71)90316-8.
- Growth and continuous passage of COMMA-D mouse mammary epithelial cells in hormonally defined serum-free medium. Riss et al. Cancer Res. 1987 Jul 15;47(14):3776-82. PMID: 3297308.
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| Parameter |
Specification |
| Appearance |
Red, clear liquid |
| pH |
7.2 ± 0.1 |
| Osmolality |
275-360 mOsm/L |
| Endotoxin |
NMT< 2EU/mL |
| Mycoplasma |
Negative |
| Suitability |
Suitable for mammalian cell culture |
| Additive |
Sodium pyruvate |
| Indicator |
Phenol red |
| Mycoplasma Detection |
Negative |
| Sterility Tested |
Sterile filtered using 0.22 µm filter |
| Form |
Liquid |
| Shipping Condition |
Room temperature |
[/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed
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