Chemically Defined Williams’ E Medium

Description

Background

PurMa™ Chemically Defined Williams’ E Medium (CD Williams’ E Medium) is a proprietary formulation used to grow cells in vitro without any undefined or animal-derived components. Moreover, CD Williams’ E Medium contains known quantities of pure chemicals, including amino acids, vitamins, salts, sugars, and more importantly a proprietary combination of synthetic growth factors.

It is absolutely crucial to mention that the PurMa™ Growth Supplement for each conventional media such as DMEM, IMDM, RPMI-1640… (base media) are slightly different in combination, amount of several growth factors, ions, chemicals, pH with particular adjustments needs to be done, based on the environments the base media provides.

This provides a consistent and controlled environment for cell growth and research. Noticeably, CD Williams’ E Medium is essential for applications that require reproducibility and regulatory compliance as well as for studying specific cellular processes, without extraneous variables. Furthermore, CD Williams’ E Medium is designed for custom formulations tailored for various cell types

Important Notes

It is crucially important to have a gradual transition from media containing FBS to chemically defined cell culture media. Follow these steps:

• Starting Point: 10% FBS+ (control) (you need two controls, one to be left in only 10% FBS and continue monitoring for comparing with 0% FBS + PurMa^™ Chemically Defined Medium. The second one is chemically defined medium + 10% FBS, which is going through the reduction of FBS process
• 48 hours later: 8% FBS in + PurMa^™ Chemically Defined Medium
• 48 hours later: 6% FBS in + PurMa^™ Chemically Defined Medium
• 48 hours later: 4% FBS + PurMa^™ Chemically Defined Medium
• 48 hours later: 2% FBS + PurMa^™ Chemically Defined Medium
• 48 hours later: 1% FBS  + PurMa^™ Chemically Defined Medium
• 24 hours later: 0% FBS and only PurMa^™ Chemically Defined Medium

Importantly, for each change, spin down and fully discard the media and replace it with the next lower percentage of FBS.

Noticeably, you don’t have to start with a huge number of cells (~500,000 cells/ml). Additionally, we strongly recommend you start with more than one plate as some of the plates might get contaminated

So, once the cells are kept in chemically defined Media, you should always keep them in the same chemically defined medium from the same vendor and the same formulation, even for freezing (see below).

Usually, after you get your cells adapted to PurMa^™ Chemically Defined Medium you need to make several plates and freeze down several vials. The mentioned cells are adapted to chemically defined medium and when you thaw them in the first week, in addition to PurMa™ Chemically Defined Medium, you need to add 10% PurMa^™ CD Booster (Cat# P3C10144).

Formulation

The CD Williams’ E Medium includes a base formulation including L-Alanine -L glutamine : Base Formulation of CD William’s E Medium, P3C310880

Additionally, CD Williams’ E Medium contains a proprietary synthetic cocktail of specific growth factors, hormones, and other nutrients which makes it independent of any serum and any trace of animal products.

References

1. Serum-free culture of rabbit meniscal fibrochondrocytes: proliferative response. Webber RJ, Zitaglio T, Hough AJ Jr. J Orthop Res. 1988;6(1):13-23. doi: 10.1002/jor.1100060103. PMID: 333473
2. Primary in vitro antibody responses by purified murine B lymphocytes in serum-free defined medium. Mosier DE. J Immunol. 1981 Oct;127(4):1490-3. PMID: 6792277

Learn more: What Is William’s E Medium?