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Leibovitz’s L-15 Medium
Leibovitz’s L-15 Medium was originally designed to be used in carbon dioxide (CO2) free systems. Therefore, does not need sodium bicarbonate to provide the buffering capacity. Instead, phosphates, free base amino acids, salts, and galactose maintain the physiological pH.
Description
Background
Leibovitz’s L-15 Medium was originally designed to be used in carbon dioxide (CO2) free systems. Therefore, it does not need sodium bicarbonate to provide the buffering capacity. Instead, phosphates, free base amino acids, salts, and galactose maintain the physiological pH.
This media contains:
- High Level of Sodium pyruvate (5mM)
- Undoubtedly, the absence of Sodium Bicarbonate makes this media unique.
- Basically, the buffering Capacity is maintained by other elements, mainly phosphates.
- And finally Using Galactose instead of glucose makes this media quite different.
Application of Leibovitz’s L-15
- Cryopreservation of cells and tissues such as Xenopus sperm and embryonic cells.
- Establishing human tumor cell lines such as Hep-2 cells.
- As a supplement in sterile cell media for the dissection and isolation of kidney cells.
- As an important media which provides a proper buffering system for culturing of lung cells.
- A valid option for growing fish cells originating from cell lines.
PurMa Biologics manufactures 107 types of Leibovitz’s L-15
Why PurMa Biologics? Why we stand behind our cell culture media
Our products are the outcome of 30 years of experience in cutting edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. We utilize 99% pure amino acids. This will significantly increase the consistency among your experiments, and the result of your lab. Considering the highest quality, our prices are extremely reasonable.
Formulation
For complete formulation please visit the “formulation tab”, as well as by clicking here: Leibovitz’s L-15 Formulation
References
- The effect of a phorbol ester on amphibian myogenesis. Zeng MB, Tseng MP. Shi Yan Sheng Wu Xue Bao. 1990 Mar;23(1):106-15. PMID: 2382522.
- Expression of differentiation molecules is preserved in human fetal kidneys during culture. Brière et al. Acta Histochem. 1990;89(2):157-66. doi: 10.1016/s0065-1281(11)80351-x.PMID: 2093265.
| Parameter | Specification |
|---|---|
| Appearance | Red, clear liquid |
| pH | 7.2 ± 0.1 |
| Osmolality | 275-360 mOsm/L |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Suitability | Suitable for mammalian cell culture |
| Additive | Sodium pyruvate |
| Indicator | Phenol red |
| Mycoplasma Detection | Negative |
| Sterility Tested | Sterile filtered using 0.22 µm filter |
| Form | Liquid |
| Shipping Condition | Room temperature |
Additional information
| Condition | Leibovitz's L-15 Standard Formulation, Leibovitz's L-15 w/o Glutamine, Leibovitz's L-15 w/o Phenol Red, Leibovitz's L-15 w/o Sodium Pyruvate, Leibovitz's L-15 w/o Galactose, Leibovitz's L-15 High Galactose (4.5 g/L), Leibovitz's L-15 15 mM HEPES (3.6 g/L), Leibovitz's L-15 25 mM HEPES (5.9 g/L) |
|---|---|
| Format | Liquid, Powder |
| Size | 10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L |
Documents
Certificate of Analysis (COA): Available upon request for your specific order — email info@purmabiologics.com and we will send the current lot documentation.
Safety Data Sheet (SDS): Available upon request — email info@purmabiologics.com.
What our customers say about PurMa Biologics
Feedback from labs we supply, across our full catalogue.
It’s been a pleasure working with PURMA Biologics. Their service is efficient, delivery is fast, and the quality of their products has met our expectations consistently. Very smooth collaboration overall.
PurMa products are of excellent quality and highly reliable.
I've ordered cell culture media multiple times. Great prices and excellent customer service. The ordering process was smooth, and everything arrived as expected. I'll definitely be purchasing from them again.
We buy all of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
We have been working with PurMa biologics for the past several years, we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum. We appreciate the high quality and reasonable price, and great customer service.
We purchase everything we need related to cell and tissue culture from PurMa, reagents like: DMEM, LL15, EMEM, FBS, trypsin, antibiotics mix100X and... Always great quality, fast processing and shipping. We are totally satisfied.
Frequently asked questions
What concentration is Leibovitz’s L-15 Medium supplied at?
Most supplements and solutions are supplied as concentrated stocks, commonly 100X, for dilution into complete medium. The exact concentration is on the Certificate of Analysis for the lot.
How should it be stored?
Follow the storage line on the Certificate of Analysis, and aliquot anything supplied frozen so you are not repeatedly freezing and thawing the stock.
Is it sterility and endotoxin tested?
Yes. Each lot is tested and a Certificate of Analysis is issued and available on request for any catalogue number.
Can I get this in a custom concentration or pack size?
Yes. Custom concentrations, formulations and bulk pack sizes are made to order. Use the request bulk pricing button on this page and state the specification you need.
What is the shelf life?
Stated per lot on the Certificate of Analysis. Once opened the practical limit is usually contamination rather than chemical stability, so handle in a hood and decant rather than pipetting repeatedly from the stock.
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