Basal Medium Eagle (BME) in Hanks’ Buffer

  • Basal Medium Eagle (BME) in Hanks’ Buffer

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    Basal Medium Eagle (BME) in Hanks’ Buffer

    Background

    Basal Medium Eagle (BME) in Hanks’ Buffer originally developed by Harry Eagle. Moreover, this media is one of the most widely used of all synthetic cell culture media. Markedly, BME is the predecessor of Eagle’s Minimum Essential Medium (MEM) and Dulbecco’s Modified Eagle’s Medium (DME). PurMa Biologics presently manufactures BME with Earle’s for use in a CO2 incubator, or with Hanks' (HBSS) salts for use without CO2.

    Ingredients

    As the composition, this media contains HBSS buffer HBSS buffer contains:
    1. Potassium Chloride
    2. Potassium Phosphate, monobasic
    3. Sodium Bicarbonate
    4. Sodium Chloride
    5. And lastly, Sodium Phosphate, dibasic

    Application

    PurMa Biologics manufactures 117 types BME in HBSS buffer. This media has been successfully used for variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts as well as  primary rat astrocytes.

    Formulation

    For complete formulation click, or alternatively  look at the “ Formulation Tab”:  BME with Hanks' Buffer (HBSS) Formulation

    References

    1. Nutritional requirements for the production of herpes simplex virus. I. Influence of glucose and glutamine of herpes simplex virus production by HeLa cells. LEWIS et al. J Bacteriol. 1962 Mar;83(3):475-82. doi: 10.1128/jb.83.3.475-482.1962. PMID: 14464909
    2. Improved chemically defined basal medium (CMRL-1969) for primary monkey kidney and human diploid cells. Healy et al. Appl Microbiol. 1971 Jan;21(1):1-5. doi: 10.1128/am.21.1.1-5.1971.
    3. Differential cytopathogenicity accompanying Mycoplasma pneumoniae infection of human lung fibroblasts maintained in newborn bovine serum or fetal bovine serum. Upchurch et al. In Vitro. 1983 Mar;19(3 Pt 1):203-9. doi: 10.1007/BF0 2618060. PMID: 6187665.
    [/fusion_text][fusion_table fusion_table_type="2" fusion_table_rows="13" fusion_table_columns="2" margin_top="30px" margin_right="" margin_bottom="" margin_left="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" class="" id="" animation_type="" animation_direction="left" animation_color="" hue="" saturation="" lightness="" alpha="" animation_speed="0.3" animation_delay="0" animation_offset=""]
    Parameter Specification
    Appearance Red, clear liquid
    pH 7.2 ± 0.1
    Osmolality 275-360 mOsm/L
    Endotoxin NMT< 2EU/mL
    Mycoplasma Negative
    Suitability  Suitable for mammalian cell culture
    Additive N/A
    Indicator Phenol red
    Mycoplasma Detection Negative
    Sterility Tested Sterile filtered using 0.22 µm filter
    Form Liquid
    Shipping Condition  Room temperature
    [/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed Read more
  • Dulbecco’s Modified Eagle’s Medium (DMEM)

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    DMEM

    Background; Dulbecco’s Modified Eagle’s Medium

    Dulbecco’s Modified Eagle’s Medium (DMEM) is a modification of Basal Medium Eagle (BME). Currently, DMEM is one of the most widely used media.

    Major modifications compared to the original formulation:

    1. Although the original Dulbecco’s Modified Eagle’s Medium contained 1000 mg/L of glucose, there are different Glucose amounts used.  As shown below, PurMa Biologics DMEM contains 4.5 g/L glucose. Additionally, PurMa Biologics offers a (1.5 g/L) low glucose (P3SO28105) condition.
    2. Furthermore, DMEM contains fourfold more amino acids and vitamins compared to the original formulation. DMEM is a good choice for growing the primary cell culture of mouse and chicken cells. Moreover, for a variety of normal and transformed cells, this media can be used properly. Each of these DMEMs, by comparison, offers a different combination of L-glutamine and sodium pyruvate.
    3. More importantly, DMEM has several fold more buffering capacities (3.7g/L NaHCO3) Need of FBS.

    Lastly, DMEM Contains:

    Furthermore, DMEM contains no proteins, lipids, or growth factors. Therefore, it requires supplementation, commonly with 10% Fetal Bovine Serum (FBS).
    • High glucose (4.5g/L)
    • Non-essential amino acids
    • Sodium pyruvate (0.11 g/L)
    • Phenol red (0.0015g/L)
    • L-glutamine (0.585 g/L)

    Types of DMEM

    Currently, PurMa Biologics Manufactures three forms of DMEM: Standard, MAX, and Advanced. Moreover, the optimization of these three forms of DMEM is the outcome of decades of cell culture research and experience.  Undoubtedly, our DMEM delivers exceptional growth and consistency for your cell culture projects.

    Formulation:

    Complete formulation is available here: DMEM Formulation

    Reference:

    1. The viability change of pigskin in vitro. Ge L, et al. 2010 Jun;36(4):533-8.
    2. A protocol for isolation and culture of mesenchymal stem cells from mouse bone marrow. Soleimani M, Nadri S.Nat Protoc. 2009;4(1):102-6.
    [/fusion_text][fusion_table fusion_table_type="1" fusion_table_rows="13" fusion_table_columns="2" margin_top="30px" margin_right="" margin_bottom="" margin_left="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" class="" id="" animation_type="" animation_direction="left" animation_color="" hue="" saturation="" lightness="" alpha="" animation_speed="0.3" animation_delay="0" animation_offset=""]
    Parameter Specification
    Appearance Red, clear liquid
    pH 7.2 ± 0.1
    Osmolality 275-360 mOsm/L
    Endotoxin NMT< 2EU/mL
    Mycoplasma Negative
    Suitability  Suitable for mammalian cell culture
    Additive Sodium pyruvate
    Indicator Phenol red
    Mycoplasma Detection Negative
    Sterility Tested Sterile filtered using 0.22 µm filter
    Form Liquid
    Shipping Condition  Room temperature
    [/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed Read more
  • Dulbecco’s Modified Eagle’s Medium/F-12 (DMEM F-12)

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    DMEM F-12

    Background

    Dulbecco’s Modified Eagle’s Medium/F-12 (DMEM F-12) provides superior quality, consistency, and great control in mammalian cell culture. DMEM F-12 is preferred for MDCK, glial cells, fibroblasts, human endothelial cells, as well as rat fibroblasts. In general, DMEM: F-12 is more enriched but contains the following major changes compared to DMEM:

    Ingredients

    • It contains 2.5 mM L-glutamine
    • 0.5 mM sodium pyruvate
    • Moreover, contains 30mM NaHCO3 (2.438g/L)
    • High glucose
    • Non-Essential Amino Acids
    • also, contains Sodium pyruvate
    • Phenol Red
    • L-glutamine

     Formulation

    For complete formulation click here: DMEM F-12 Formultaion

    References:

    1.  Clonal growth of mammalian cells in a chemically defined, synthetic medium . DULBECCO et al. 1959 Jul;8(3):396-7. doi: 10.1016 /0042-68 22(59)90043-1. PMID: 13669362.
    2. Plaque production by the polyoma virus. DULBECCO et al. 1959 Jul;8(3):396-7. doi: 10.1016/0042-6822(59)90043-1. PMID: 1366936.
    [/fusion_text][fusion_table fusion_table_type="1" fusion_table_rows="13" fusion_table_columns="2" margin_top="30px" margin_right="" margin_bottom="" margin_left="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" class="" id="" animation_type="" animation_direction="left" animation_color="" hue="" saturation="" lightness="" alpha="" animation_speed="0.3" animation_delay="0" animation_offset=""]
    Parameter Specification
    Appearance Red, clear liquid
    pH 7.2 ± 0.1
    Osmolality 275-360 mOsm/L
    Endotoxin NMT< 2EU/mL
    Mycoplasma Negative
    Suitability  Suitable for mammalian cell culture
    Additive Sodium pyruvate
    Indicator Phenol red
    Mycoplasma Detection Negative
    Sterility Tested Sterile filtered using 0.22 µm filter
    Form Liquid
    Shipping Condition  Room temperature
    [/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed Read more
  • Eagle’s Minimal Essential Medium (EMEM)

    [fusion_builder_container type="flex" hundred_percent="no" hundred_percent_height="no" min_height_medium="" min_height_small="" min_height="" hundr... [fusion_builder_container type="flex" hundred_percent="no" hundred_percent_height="no" min_height_medium="" min_height_small="" min_height="" hundred_percent_height_scroll="no" align_content="stretch" flex_align_items="flex-start" flex_justify_content="flex-start" flex_column_spacing="" hundred_percent_height_center_content="yes" equal_height_columns="no" container_tag="div" menu_anchor="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" status="published" publish_date="" class="" id="" spacing_medium="" margin_top_medium="" margin_bottom_medium="" spacing_small="" margin_top_small="" margin_bottom_small="" margin_top="" margin_bottom="" padding_dimensions_medium="" padding_top_medium="" padding_right_medium="" padding_bottom_medium="" padding_left_medium="" padding_dimensions_small="" padding_top_small="" padding_right_small="" padding_bottom_small="" padding_left_small="" padding_top="" padding_right="" padding_bottom="" padding_left="" link_color="" 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filter_sepia_hover="0" filter_opacity_hover="100" filter_blur_hover="0"][fusion_builder_row][fusion_builder_column type="1_1" layout="1_1" align_self="auto" content_layout="column" align_content="flex-start" valign_content="flex-start" content_wrap="wrap" spacing="" center_content="no" column_tag="div" link="" target="_self" link_description="" min_height="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" sticky_display="normal,sticky" class="" id="" type_medium="" type_small="" order_medium="0" order_small="0" dimension_spacing_medium="" dimension_spacing_small="" dimension_spacing="" dimension_margin_medium="" dimension_margin_small="" margin_top="" margin_bottom="" padding_medium="" padding_small="" padding_top="" padding_right="" padding_bottom="" padding_left="" hover_type="none" border_sizes="" border_color="" border_style="solid" border_radius="" box_shadow="no" dimension_box_shadow="" box_shadow_blur="0" box_shadow_spread="0" box_shadow_color="" 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filter_opacity_hover="100" filter_blur_hover="0" transform_type="regular" transform_hover_element="self" transform_scale_x="1" transform_scale_y="1" transform_translate_x="0" transform_translate_y="0" transform_rotate="0" transform_skew_x="0" transform_skew_y="0" transform_scale_x_hover="1" transform_scale_y_hover="1" transform_translate_x_hover="0" transform_translate_y_hover="0" transform_rotate_hover="0" transform_skew_x_hover="0" transform_skew_y_hover="0" transform_origin="" transition_duration="300" transition_easing="ease" transition_custom_easing="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset="" last="true" border_position="all" first="true"][fusion_text columns="" column_min_width="" column_spacing="" rule_style="" rule_size="" rule_color="" hue="" saturation="" lightness="" alpha="" content_alignment_medium="" content_alignment_small="" content_alignment="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" sticky_display="normal,sticky" class="" id="" margin_top="" margin_right="" margin_bottom="" margin_left="" fusion_font_family_text_font="" fusion_font_variant_text_font="" font_size="" line_height="" letter_spacing="" text_transform="" text_color="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset=""]

    Eagle’s Minimum Essential Medium (EMEM)

    Background

    Eagle's Minimal Essential Medium (EMEM) developed by Harry Eagle and is widely used. It is composed of necessary salts for optimal cell growth and maintenance. Additionally, it contains high glucose, essential amino acids, and vitamins. Ever since, several versions of EMEM have been developed by modification of its composition. This media has is suitable for a wide range of mammalian cells, adhesive as well as suspension cells.

    Ingridients

    Moreover, PurMa TM EMEM Medium contains:
    • Low level of glucose (1.00 g/ liter)
    • Sodium bicarbonate (1.5 g/L)
    • Additionally, PurMa Biologics also offers EMEM Medium with high sodium bicarbonate (2.438 g/L) (Cat # P3S032110)
    • 2 mM L-glutamine (292 mg/L)
    • 1mM sodium pyruvate (0.11g/L)
    • Additionally, a higher concentration of amino acids, vitamins, inositol and choline are present in this medium.
    PurMa Biologics manufactures 106 types of EMEM which accommodate any form of this medium. We recommend contacting our friendly customer service & technical support about your exact need for EMEM media.

    Why PurMa Biologics? Why we stand behind our cell culture media

    Our products are the outcome of 30 years of experience in cutting edge cell and tissue culture science. We routinely manufacture over 1500 media which is the most comprehensive collection of cell and tissue culture media. We utilize %99 pure amino acids. This will significantly increase the consistency among your experiments, and the result of your lab compared to other laboratories working on the same issues. Considering the highest quality, our prices are extremely reasonable.

    Application of EMEM

    Additionally, this media has elements that prevent clumping. This property certainly makes this media suitable for growing tumor cells as well as hybridoma cells lines. The dedicated scientists in PurMa Biologics have decades of experience with this media for culturing primary as well as commercial cell lines including human Osteosarcoma, Hek293 Cell, HeLa, Jurkat, MG63 cells, MCF-7, PC12, PBMC, and hybridoma cells.

    Formulation

    for complete formulation click here:  EMEM Formultation  References
    1. Effects of assay medium composition on macrophage-mediated tumor cell binding and lysis. Hasday et al. J Immunol Methods. 1988 Nov 10;114(1-2):243-52. doi: 10.1016/0022-1759(88)90180-9. PMID: 3141516.
    2. Comparative activity in different media of ketoconazole, miconazole and amphotericin B against Candida lusitaniae and sucrose-negative Candida tropicalis. Mignini et al. Chemioterapia. 1988 Feb;7(1):38-41. PMID: 3378274.
    3. Dextran T-500 improves survival and spreading of chick embryo cells in serum-free medium. Pietrzkowski et al. Folia Histochem Cytobiol. 1988;26(3):123-31. PMID: 2461881.
    [/fusion_text][fusion_table fusion_table_type="2" fusion_table_rows="13" fusion_table_columns="2" margin_top="30px" margin_right="" margin_bottom="" margin_left="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" class="" id="" animation_type="" animation_direction="left" animation_color="" hue="" saturation="" lightness="" alpha="" animation_speed="0.3" animation_delay="0" animation_offset=""]
    Parameter Specification
    Appearance Red, clear liquid
    pH  7.2 ± 0.1
    Osmolality  275-360 mOsm/L
    Endotoxin  NMT< 2EU/mL
    Mycoplasma Negative
    Suitability  Suitable for mammalian cell culture
    Additive  Sodium pyruvate
    Indicator  Phenol red
    Mycoplasma Detection Negative
    Sterility Tested  Sterile filtered using 0.22 µm filter
    Form Liquid
    Shipping Condition  Room temperature
    [/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed Read more
  • Glasgow’s Modified Eagle’s Medium

    [fusion_builder_container type="flex" hundred_percent="no" hundred_percent_height="no" min_height_medium="" min_height_small="" min_height="" hundr... 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pattern_custom_bg="" pattern_bg_color="" pattern_bg_style="default" pattern_bg_opacity="100" pattern_bg_size="" pattern_bg_blend_mode="normal" mask_bg="none" mask_custom_bg="" mask_bg_color="" mask_bg_accent_color="" mask_bg_style="default" mask_bg_opacity="100" mask_bg_transform="left" mask_bg_blend_mode="normal" render_logics="" absolute="off" absolute_devices="small,medium,large" sticky="off" sticky_devices="small-visibility,medium-visibility,large-visibility" sticky_background_color="" sticky_height="" sticky_offset="" sticky_transition_offset="0" scroll_offset="0" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset="" filter_hue="0" filter_saturation="100" filter_brightness="100" filter_contrast="100" filter_invert="0" filter_sepia="0" filter_opacity="100" filter_blur="0" filter_hue_hover="0" filter_saturation_hover="100" filter_brightness_hover="100" filter_contrast_hover="100" filter_invert_hover="0" filter_sepia_hover="0" filter_opacity_hover="100" filter_blur_hover="0"][fusion_builder_row][fusion_builder_column type="1_1" layout="1_1" align_self="auto" content_layout="column" align_content="flex-start" valign_content="flex-start" content_wrap="wrap" spacing="" center_content="no" column_tag="div" link="" target="_self" link_description="" min_height="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" sticky_display="normal,sticky" class="" id="" type_medium="" type_small="" order_medium="0" order_small="0" dimension_spacing_medium="" dimension_spacing_small="" dimension_spacing="" dimension_margin_medium="" dimension_margin_small="" margin_top="" margin_bottom="" padding_medium="" padding_small="" padding_top="" padding_right="" padding_bottom="" padding_left="" hover_type="none" border_sizes="" border_color="" border_style="solid" border_radius="" box_shadow="no" dimension_box_shadow="" box_shadow_blur="0" box_shadow_spread="0" box_shadow_color="" box_shadow_style="" z_index_subgroup="regular" z_index="" z_index_hover="" overflow="" background_type="single" gradient_start_color="" gradient_end_color="" gradient_start_position="0" gradient_end_position="100" gradient_type="linear" radial_direction="center center" linear_angle="180" background_color="" background_image="" background_image_id="" lazy_load="none" skip_lazy_load="" background_position="left top" background_repeat="no-repeat" background_blend_mode="none" render_logics="" sticky="off" sticky_devices="small-visibility,medium-visibility,large-visibility" sticky_offset="" absolute="off" absolute_props="" filter_type="regular" filter_hover_element="self" filter_hue="0" filter_saturation="100" filter_brightness="100" filter_contrast="100" filter_invert="0" filter_sepia="0" filter_opacity="100" filter_blur="0" filter_hue_hover="0" filter_saturation_hover="100" filter_brightness_hover="100" filter_contrast_hover="100" filter_invert_hover="0" filter_sepia_hover="0" filter_opacity_hover="100" filter_blur_hover="0" transform_type="regular" transform_hover_element="self" transform_scale_x="1" transform_scale_y="1" transform_translate_x="0" transform_translate_y="0" transform_rotate="0" transform_skew_x="0" transform_skew_y="0" transform_scale_x_hover="1" transform_scale_y_hover="1" transform_translate_x_hover="0" transform_translate_y_hover="0" transform_rotate_hover="0" transform_skew_x_hover="0" transform_skew_y_hover="0" transform_origin="" transition_duration="300" transition_easing="ease" transition_custom_easing="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset="" last="true" border_position="all" first="true"][fusion_text columns="" column_min_width="" column_spacing="" rule_style="" rule_size="" rule_color="" hue="" saturation="" lightness="" alpha="" content_alignment_medium="" content_alignment_small="" content_alignment="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" sticky_display="normal,sticky" class="" id="" margin_top="" margin_right="" margin_bottom="" margin_left="" fusion_font_family_text_font="" fusion_font_variant_text_font="" font_size="" line_height="" letter_spacing="" text_transform="" text_color="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset=""]

    Glasgow’s Modified Eagle’s Medium

    Background

    Ian McPherson and Michael Stoker developed Glasgow’s Modified Eagle’s Medium . they used  polyoma virus to transform four fibroblast clones from a culture of baby hamster kidney cells.  as a result to Particular criteria mentioned below Glasgow Minimum Essential Medium (GMEM) is used without serum. Needs to be supplemented with 10% Tryptose phosphate broth. Moreover, it contains twice amount of amino acids and vitamins compared to the original Basal Medium Eagle.

    PurMa™ Glasgow’s Modified Eagle’s Medium also Contains

    • 75 g/L Sodium Bicarbonate
    • High Glucose (4.5g/L)
    PurMa Biologics manufactures 77 types of GMEM

    Why PurMa Biologics? Why we stand behind our cell culture media

    Our products are the outcome of 30 years of experience in cutting edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. We utilize %99 pure amino acids. This will significantly increase the consistency among your experiments, and the result of your lab. Considering the highest quality, our prices are extremely reasonable.

    Application of this media

    It is suitable for growth of primate embryonic stem cells, MDCK, NS0, BHK-21 as well as CHO cells containing the GS expression system. Alternative to 10% Tryptose phosphate broth, we strongly recommend PurMa TM 50X GS Supplement. This is certainly a enriched mixture of amino acids and nucleosides, and Fetal Bovine Serum (FBS) (Cat P3S528332). furthermore, this media has been formulated for use in a 5% carbon dioxide atmosphere. As a result, It utilizes a bicarbonate buffering system.

    Formulation

    The Complete formulation is available in " formulation tab" as well as by clicking here: Glasgow’s (GMEM) Formulation

    References

    [/fusion_text][fusion_table fusion_table_type="2" fusion_table_rows="13" fusion_table_columns="2" margin_top="30px" margin_right="" margin_bottom="" margin_left="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" class="" id="" animation_type="" animation_direction="left" animation_color="" hue="" saturation="" lightness="" alpha="" animation_speed="0.3" animation_delay="0" animation_offset=""]
    Parameter Specification
    Appearance Red, clear liquid
    pH 7.2 ± 0.1
    Osmolality 275-360 mOsm/L
    Endotoxin NMT< 2EU/mL
    Mycoplasma Negative
    Suitability  Suitable for mammalian cell culture
    Additive Sodium pyruvate
    Indicator Phenol red
    Mycoplasma Detection Negative
    Sterility Tested Sterile filtered using 0.22 µm filter
    Form Liquid
    Shipping Condition  Room temperature
    [/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed Read more
  • Iscove’s Modified Dulbecco’s Medium (IMDM)

    [fusion_builder_container type="flex" hundred_percent="no" hundred_percent_height="no" min_height_medium="" min_height_small="" min_height="" hundr... 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pattern_custom_bg="" pattern_bg_color="" pattern_bg_style="default" pattern_bg_opacity="100" pattern_bg_size="" pattern_bg_blend_mode="normal" mask_bg="none" mask_custom_bg="" mask_bg_color="" mask_bg_accent_color="" mask_bg_style="default" mask_bg_opacity="100" mask_bg_transform="left" mask_bg_blend_mode="normal" render_logics="" absolute="off" absolute_devices="small,medium,large" sticky="off" sticky_devices="small-visibility,medium-visibility,large-visibility" sticky_background_color="" sticky_height="" sticky_offset="" sticky_transition_offset="0" scroll_offset="0" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset="" filter_hue="0" filter_saturation="100" filter_brightness="100" filter_contrast="100" filter_invert="0" filter_sepia="0" filter_opacity="100" filter_blur="0" filter_hue_hover="0" filter_saturation_hover="100" filter_brightness_hover="100" filter_contrast_hover="100" filter_invert_hover="0" filter_sepia_hover="0" filter_opacity_hover="100" filter_blur_hover="0"][fusion_builder_row][fusion_builder_column type="1_1" layout="1_1" align_self="auto" content_layout="column" align_content="flex-start" valign_content="flex-start" content_wrap="wrap" spacing="" center_content="no" column_tag="div" link="" target="_self" link_description="" min_height="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" sticky_display="normal,sticky" class="" id="" type_medium="" type_small="" order_medium="0" order_small="0" dimension_spacing_medium="" dimension_spacing_small="" dimension_spacing="" dimension_margin_medium="" dimension_margin_small="" margin_top="" margin_bottom="" padding_medium="" padding_small="" padding_top="" padding_right="" padding_bottom="" padding_left="" hover_type="none" border_sizes="" border_color="" border_style="solid" border_radius="" box_shadow="no" dimension_box_shadow="" box_shadow_blur="0" box_shadow_spread="0" box_shadow_color="" box_shadow_style="" z_index_subgroup="regular" z_index="" z_index_hover="" overflow="" background_type="single" gradient_start_color="" gradient_end_color="" gradient_start_position="0" gradient_end_position="100" gradient_type="linear" radial_direction="center center" linear_angle="180" background_color="" background_image="" background_image_id="" lazy_load="none" skip_lazy_load="" background_position="left top" background_repeat="no-repeat" background_blend_mode="none" render_logics="" sticky="off" sticky_devices="small-visibility,medium-visibility,large-visibility" sticky_offset="" absolute="off" absolute_props="" filter_type="regular" filter_hover_element="self" filter_hue="0" filter_saturation="100" filter_brightness="100" filter_contrast="100" filter_invert="0" filter_sepia="0" filter_opacity="100" filter_blur="0" filter_hue_hover="0" filter_saturation_hover="100" filter_brightness_hover="100" filter_contrast_hover="100" filter_invert_hover="0" filter_sepia_hover="0" filter_opacity_hover="100" filter_blur_hover="0" transform_type="regular" transform_hover_element="self" transform_scale_x="1" transform_scale_y="1" transform_translate_x="0" transform_translate_y="0" transform_rotate="0" transform_skew_x="0" transform_skew_y="0" transform_scale_x_hover="1" transform_scale_y_hover="1" transform_translate_x_hover="0" transform_translate_y_hover="0" transform_rotate_hover="0" transform_skew_x_hover="0" transform_skew_y_hover="0" transform_origin="" transition_duration="300" transition_easing="ease" transition_custom_easing="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset="" last="true" border_position="all" first="true"][fusion_text columns="" column_min_width="" column_spacing="" rule_style="" rule_size="" rule_color="" hue="" saturation="" lightness="" alpha="" content_alignment_medium="" content_alignment_small="" content_alignment="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" sticky_display="normal,sticky" class="" id="" margin_top="" margin_right="" margin_bottom="" margin_left="" fusion_font_family_text_font="" fusion_font_variant_text_font="" font_size="" line_height="" letter_spacing="" text_transform="" text_color="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset=""]

    Iscove's Modified Dulbecco's Medium (IMDM)

    Background

    Iscove's Modified Dulbecco's Medium (IMDM) is specialized for rapidly proliferating, high-density cell lines. Therefore, it is excellent for Jurkat, Hek, COS-7, and macrophage cells. IMDM is a modified and more advanced version of DMEM. Additionally, it includes selenium as well as additional amino acids and vitamins. it is the only medium that lacks Iron, with potassium nitrate replacing ferric nitrate, therefore it makes it excellent  for adhesive cells.

    Ingredients

    It contains:
    • High Buffering Capacity with High NaHCO3 as well as  High HEPES
    • Sodium Bicarbonate (3.02 g/L)
    • 5.9 g/L HEPES
    • High glucose 4.5g/L
    • Non-Essential Amino Acids
    • 110 mg/L Sodium pyruvate (1.0 mM)
    • 15 mg/L Phenol Red
    • 0.584 g/L L-glutamine

    Formulation

    Complete formulation is available on our website as well as here : IMDM Formulation 

    References

    1. Plaque production by the polyoma virus. DULBECCO R, FREEMAN G. 1959 Jul;8(3):396-7. doi: 10.1016/0042-6822(59)90043-1. PMID: 13669362.
    2. Complete replacement of serum by albumin, transferrin, and soybean lipid in cultures of lipopolysaccharide-reactive B lymphocytes. J Exp Med. 1978 Mar 1; 147(3): 923–933. doi: 10.1084/jem.147.3.923 PMCID: PMC2184195
    [/fusion_text][fusion_table fusion_table_type="2" fusion_table_rows="13" fusion_table_columns="2" margin_top="30px" margin_right="" margin_bottom="" margin_left="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" class="" id="" animation_type="" animation_direction="left" animation_color="" hue="" saturation="" lightness="" alpha="" animation_speed="0.3" animation_delay="0" animation_offset=""]
    Parameter Specification
    Appearance Red, clear liquid
    pH  7.2 ± 0.1
    Osmolality  275-360 mOsm/L
    Endotoxin  NMT< 2EU/mL
    Mycoplasma Negative
    Suitability  Suitable for mammalian cell culture
    Additive  Sodium pyruvate
    Indicator  Phenol red
    Mycoplasma Detection Negative
    Sterility Tested  Sterile filtered using 0.22 µm filter
    Form Liquid
    Shipping Condition  Room temperature
    [/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed Read more
  • Leibovitz’s L-15 Medium

    [fusion_builder_container type="flex" hundred_percent="no" hundred_percent_height="no" min_height_medium="" min_height_small="" min_height="" hundr... 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hide_on_mobile="small-visibility,medium-visibility,large-visibility" sticky_display="normal,sticky" class="" id="" margin_top="" margin_right="" margin_bottom="" margin_left="" fusion_font_family_text_font="" fusion_font_variant_text_font="" font_size="" line_height="" letter_spacing="" text_transform="" text_color="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset=""]

    Leibovitz's L-15 Medium

    Background

    Leibovitz's L-15 Medium was originally designed to be used in carbon dioxide (CO2) free systems. Therefore, does not need sodium bicarbonate to provide the buffering capacity. Instead, phosphates, free base amino acids, salts, and galactose maintain the physiological pH.

    This media contains:

    • High Level of Sodium pyruvate (5mM)
    • Undoubtedly, the absence of Sodium Bicarbonate makes this media unique.
    • Basically, the buffering Capacity is maintained by other elements, mainly phosphates.
    • And finally Using Galactose instead of glucose makes this media quite different.

    Application of Leibovitz's L-15

    • Cryopreservation of cells and tissues such as Xenopus sperm and embryonic cells.
    • Establishing human tumor cells lines such as Hep-2 cells.
    • As a supplement in sterile cell media for the dissection and isolation of kidney cells.
    • As an important media which provides proper buffering system for culturing of lung cells.
    • A valid option for growing fish originated from cell lines.
    PurMa Biologics manufactures 107 types of Leibovitz's L-15

    Why PurMa Biologics? Why we stand behind our cell culture media

    Our products are the outcome of 30 years of experience in cutting edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. We utilize %99 pure amino acids. This will significantly increase the consistency among your experiments, and the result of your lab. Considering the highest quality, our prices are extremely reasonable.

    Formulation

    For complete formulation please visit the "formulation tab", as well as by clicking here: Leibovitz's L-15 Formulation

    References

    1. The effect of a phorbol ester on amphibian myogenesis. Zeng MB, Tseng MP. Shi Yan Sheng Wu Xue Bao. 1990 Mar;23(1):106-15. PMID: 2382522.
    2. Expression of differentiation molecules is preserved in human fetal kidneys during culture. Brière et al. Acta Histochem. 1990;89(2):157-66. doi: 10.1016/s0065-1281(11)80351-x.PMID: 2093265.
    [/fusion_text][fusion_table fusion_table_type="2" fusion_table_rows="13" fusion_table_columns="2" margin_top="30px" margin_right="" margin_bottom="" margin_left="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" class="" id="" animation_type="" animation_direction="left" animation_color="" hue="" saturation="" lightness="" alpha="" animation_speed="0.3" animation_delay="0" animation_offset=""]
    Parameter Specification
    Appearance Red, clear liquid
    pH 7.2 ± 0.1
    Osmolality 275-360 mOsm/L
    Endotoxin NMT< 2EU/mL
    Mycoplasma Negative
    Suitability  Suitable for mammalian cell culture
    Additive Sodium pyruvate
    Indicator Phenol red
    Mycoplasma Detection Negative
    Sterility Tested Sterile filtered using 0.22 µm filter
    Form Liquid
    Shipping Condition  Room temperature
    [/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed Read more
  • McCoy’s 5A Modified Media (M5AMM)

    [fusion_builder_container type="flex" hundred_percent="no" hundred_percent_height="no" min_height_medium="" min_height_small="" min_height="" hundr... 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pattern_custom_bg="" pattern_bg_color="" pattern_bg_style="default" pattern_bg_opacity="100" pattern_bg_size="" pattern_bg_blend_mode="normal" mask_bg="none" mask_custom_bg="" mask_bg_color="" mask_bg_accent_color="" mask_bg_style="default" mask_bg_opacity="100" mask_bg_transform="left" mask_bg_blend_mode="normal" render_logics="" absolute="off" absolute_devices="small,medium,large" sticky="off" sticky_devices="small-visibility,medium-visibility,large-visibility" sticky_background_color="" sticky_height="" sticky_offset="" sticky_transition_offset="0" scroll_offset="0" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset="" filter_hue="0" filter_saturation="100" filter_brightness="100" filter_contrast="100" filter_invert="0" filter_sepia="0" filter_opacity="100" filter_blur="0" filter_hue_hover="0" filter_saturation_hover="100" filter_brightness_hover="100" filter_contrast_hover="100" filter_invert_hover="0" filter_sepia_hover="0" filter_opacity_hover="100" filter_blur_hover="0"][fusion_builder_row][fusion_builder_column type="1_1" layout="1_1" align_self="auto" content_layout="column" align_content="flex-start" valign_content="flex-start" content_wrap="wrap" spacing="" center_content="no" column_tag="div" link="" target="_self" link_description="" min_height="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" sticky_display="normal,sticky" class="" id="" type_medium="" type_small="" order_medium="0" order_small="0" dimension_spacing_medium="" dimension_spacing_small="" dimension_spacing="" dimension_margin_medium="" dimension_margin_small="" margin_top="" margin_bottom="" padding_medium="" padding_small="" padding_top="" padding_right="" padding_bottom="" padding_left="" hover_type="none" border_sizes="" border_color="" border_style="solid" border_radius="" box_shadow="no" dimension_box_shadow="" box_shadow_blur="0" box_shadow_spread="0" box_shadow_color="" box_shadow_style="" z_index_subgroup="regular" z_index="" z_index_hover="" overflow="" background_type="single" gradient_start_color="" gradient_end_color="" gradient_start_position="0" gradient_end_position="100" gradient_type="linear" radial_direction="center center" linear_angle="180" background_color="" background_image="" background_image_id="" lazy_load="none" skip_lazy_load="" background_position="left top" background_repeat="no-repeat" background_blend_mode="none" render_logics="" sticky="off" sticky_devices="small-visibility,medium-visibility,large-visibility" sticky_offset="" absolute="off" absolute_props="" filter_type="regular" filter_hover_element="self" filter_hue="0" filter_saturation="100" filter_brightness="100" filter_contrast="100" filter_invert="0" filter_sepia="0" filter_opacity="100" filter_blur="0" filter_hue_hover="0" filter_saturation_hover="100" filter_brightness_hover="100" filter_contrast_hover="100" filter_invert_hover="0" filter_sepia_hover="0" filter_opacity_hover="100" filter_blur_hover="0" transform_type="regular" transform_hover_element="self" transform_scale_x="1" transform_scale_y="1" transform_translate_x="0" transform_translate_y="0" transform_rotate="0" transform_skew_x="0" transform_skew_y="0" transform_scale_x_hover="1" transform_scale_y_hover="1" transform_translate_x_hover="0" transform_translate_y_hover="0" transform_rotate_hover="0" transform_skew_x_hover="0" transform_skew_y_hover="0" transform_origin="" transition_duration="300" transition_easing="ease" transition_custom_easing="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset="" last="true" border_position="all" first="true"][fusion_text columns="" column_min_width="" column_spacing="" rule_style="" rule_size="" rule_color="" hue="" saturation="" lightness="" alpha="" content_alignment_medium="" content_alignment_small="" content_alignment="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" sticky_display="normal,sticky" class="" id="" margin_top="" margin_right="" margin_bottom="" margin_left="" fusion_font_family_text_font="" fusion_font_variant_text_font="" font_size="" line_height="" letter_spacing="" text_transform="" text_color="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset=""]

    McCoy’s 5A Modified Media (M5AMM)

    Background

    McCoy’s 5A Modified Media (M5AMM) was originally used for Walker Carcinosarcoma 256 Cells. It was subsequently modified to create a new medium known as McCoy’s 5A. Additionally, M5AMM contains several folds of amino acids and vitamins compared to the original formulation. Our McCoy’s 5A covers the main needs for your cell lines, delivers consistency, excellent quality, and the and the full control you need for mammalian cell culture. MCCOY’S 5A Contains:
    • High glucose (3g/L)
    • Non-Essential Amino Acids
    • Phenol Red
    • L-glutamine

    Why PurMa Biologics? Why we stand behind our cell culture media:

    Our products are the outcome of 30 years of experience in cutting edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. We utilize %99 pure amino acids. This will significantly increase the consistency among your experiments, and the result of your lab. Considering the highest quality, our prices are extremely reasonable.

    Application

    McCoy’s 5A Medium is widely used for commercial cell line as CHO, HEK 293, HeLa as wellas  Jurkat cells. Moreover, PurMaTM McCoy’s Medium contains no proteins, lipids, or growth factors. Therefore, Ham’s F-12 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS).

    Formulation

    complete formulation is available in formulation tab as well as here:McCoy's 5A Formulation

    References

    1. Investigations on in vitro survival and virulence of T. pallidum under aerobiosis. Rathlev T. Br J Vener Dis. 1975 Oct;51(5):296-300. doi: 10.1136/sti.51.5.296. PMID: 172190
    2. Mycoplasmal infection of insect cell cultures. Steiner et al.In Vitro. 1983 Sep;19(9):672-82. doi: 10.1007/BF02628958.PMID: 6413389.
    3. In vitro culture of postimplantation hamster embryos. Ebron-et al. Reprod Toxicol. 1988;2(1):31-6. doi: 10.1016/s0890-6238(88)80006-6.PMID: 2980399
    [/fusion_text][fusion_table fusion_table_type="2" fusion_table_rows="13" fusion_table_columns="2" margin_top="30px" margin_right="" margin_bottom="" margin_left="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" class="" id="" animation_type="" animation_direction="left" animation_color="" hue="" saturation="" lightness="" alpha="" animation_speed="0.3" animation_delay="0" animation_offset=""]
    Parameter Specification
    Appearance Red, clear liquid
    pH  7.2 ± 0.1
    Osmolality  275-360 mOsm/L
    Endotoxin  NMT< 2EU/mL
    Mycoplasma Negative
    Suitability  Suitable for mammalian cell culture
    Additive  Sodium pyruvate
    Indicator  Phenol red
    Mycoplasma Detection Negative
    Sterility Tested  Sterile filtered using 0.22 µm filter
    Form Liquid
    Shipping Condition  Room temperature
    [/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed Read more
  • MEM Alpha Modification (MEM-α) in Hanks’ Buffer

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filter_sepia_hover="0" filter_opacity_hover="100" filter_blur_hover="0"][fusion_builder_row][fusion_builder_column type="1_1" layout="1_1" align_self="auto" content_layout="column" align_content="flex-start" valign_content="flex-start" content_wrap="wrap" spacing="" center_content="no" column_tag="div" link="" target="_self" link_description="" min_height="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" sticky_display="normal,sticky" class="" id="" type_medium="" type_small="" order_medium="0" order_small="0" dimension_spacing_medium="" dimension_spacing_small="" dimension_spacing="" dimension_margin_medium="" dimension_margin_small="" margin_top="" margin_bottom="" padding_medium="" padding_small="" padding_top="" padding_right="" padding_bottom="" padding_left="" hover_type="none" border_sizes="" border_color="" border_style="solid" border_radius="" box_shadow="no" dimension_box_shadow="" box_shadow_blur="0" box_shadow_spread="0" box_shadow_color="" box_shadow_style="" z_index_subgroup="regular" z_index="" z_index_hover="" overflow="" background_type="single" gradient_start_color="" gradient_end_color="" gradient_start_position="0" gradient_end_position="100" gradient_type="linear" radial_direction="center center" linear_angle="180" background_color="" background_image="" background_image_id="" lazy_load="none" skip_lazy_load="" background_position="left top" background_repeat="no-repeat" background_blend_mode="none" render_logics="" sticky="off" sticky_devices="small-visibility,medium-visibility,large-visibility" sticky_offset="" absolute="off" absolute_props="" filter_type="regular" filter_hover_element="self" filter_hue="0" filter_saturation="100" filter_brightness="100" filter_contrast="100" filter_invert="0" filter_sepia="0" filter_opacity="100" filter_blur="0" filter_hue_hover="0" filter_saturation_hover="100" filter_brightness_hover="100" filter_contrast_hover="100" filter_invert_hover="0" filter_sepia_hover="0" filter_opacity_hover="100" filter_blur_hover="0" transform_type="regular" transform_hover_element="self" transform_scale_x="1" transform_scale_y="1" transform_translate_x="0" transform_translate_y="0" transform_rotate="0" transform_skew_x="0" transform_skew_y="0" transform_scale_x_hover="1" transform_scale_y_hover="1" transform_translate_x_hover="0" transform_translate_y_hover="0" transform_rotate_hover="0" transform_skew_x_hover="0" transform_skew_y_hover="0" transform_origin="" transition_duration="300" transition_easing="ease" transition_custom_easing="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset="" last="true" border_position="all" first="true"][fusion_text columns="" column_min_width="" column_spacing="" rule_style="" rule_size="" rule_color="" hue="" saturation="" lightness="" alpha="" content_alignment_medium="" content_alignment_small="" content_alignment="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" sticky_display="normal,sticky" class="" id="" margin_top="" margin_right="" margin_bottom="" margin_left="" fusion_font_family_text_font="" fusion_font_variant_text_font="" font_size="" line_height="" letter_spacing="" text_transform="" text_color="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset=""]

    MEM Alpha Modification (MEM-α) in Hanks’ Buffer

    Background

    MEM Alpha Modification (MEM-α) in Hanks’ Buffer is certainly a suitable choice for mammalian cell culture as well as selection for transfected DHFR-negative cells. This media can also be used with a variety of suspension and adherent cells including keratinocytes, primary rat astrocytes, and human melanoma cells.  furthermore it is important that eliminating calcium in this media facilitates the growth of cells in suspension cultures.

    Ingredients

    MEM -α has improved regular MEM as it contains:
    • Non-essential amino acids.
    • Sodium pyruvate.
    • Lipoic acid.
    • Vitamin B12.
    • Biotin and,
    • Ascorbic acid.
    • Also, MEM-α does not contain deoxyribonucleosides and ribonucleosides.

    PurMa™ MEM -α media in Hank’s buffer includes HBSS

    Composition of HBSS buffer contains:
    • Potassium Chloride
    • Potassium Phosphate, monobasic
    • Sodium Bicarbonate
    • Sodium Chloride
    • Sodium Phosphate, dibasic
    • Low level of glucose (1.00 g/ liter)
    • Sodium bicarbonate (0.35 g/L)
    • 2 mM L-glutamine (292 mg/L)
    • Low level of glucose (1.00 g/ liter)
    Furthermore, PurMa Biologics manufactures 123 types of MEM with HBSS buffer

    Why PurMa Biologics? Why we stand behind our cell culture media

    Our products are the outcome of 30 years of experience in cutting edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. Moreover, We utilize %99 pure amino acids. This will significantly increase the consistency among your experiments. Considering the highest quality, our prices certainly are extremely reasonabl

    Application

    Alpha modification of Minimum Essential Medium Eagle certainly makes an excellent for the isolation of mesenchymal stem cells, adipose-derived stem cells as well as osteoblast. MEM -α Modification is widely used for primary osteoblast cultures as well as isolation of polymorphonuclear leukocytes (PMN) from umbilical cord blood.

    Formulation

    to get access to detailed formulation visit " formulation Tab" as well as by clicking here : MEM-alpha Formulation in Hank's Buffer (HBSS)

    References

    1. The growth of L-cells and Vero cells on an autoclavable MEM-peptone medium. Keay L.  Biotechnology and bioengineering, 19(3), 399-411 (1977-03-01).
    2. Sphingosine-1-phosphate lyase (SGPL1) deficiency is associated with mitochondrial dysfunction. Maharaj et al. The Journal of steroid biochemistry and molecular biology, 202, 105730-105730 (2020-07-20)
    [/fusion_text][fusion_table fusion_table_type="2" fusion_table_rows="13" fusion_table_columns="2" margin_top="30px" margin_right="" margin_bottom="" margin_left="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" class="" id="" animation_type="" animation_direction="left" animation_color="" hue="" saturation="" lightness="" alpha="" animation_speed="0.3" animation_delay="0" animation_offset=""]
    Parameter Specification
    Appearance Red, clear liquid
    pH 7.2 ± 0.1
    Osmolality 275-360 mOsm/L
    Endotoxin NMT< 2EU/mL
    Mycoplasma Negative
    Suitability  Suitable for mammalian cell culture
    Additive Sodium pyruvate
    Indicator Phenol red
    Mycoplasma Detection Negative
    Sterility Tested Sterile filtered using 0.22 µm filter
    Form Liquid
    Shipping Condition  Room temperature
    [/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed Read more
  • MEM Alpha Modification in Earle’s Buffer (MEM-α)

    [fusion_builder_container type="flex" hundred_percent="no" hundred_percent_height="no" min_height_medium="" min_height_small="" min_height="" hundr... [fusion_builder_container type="flex" hundred_percent="no" hundred_percent_height="no" min_height_medium="" min_height_small="" min_height="" hundred_percent_height_scroll="no" align_content="stretch" flex_align_items="flex-start" flex_justify_content="flex-start" flex_column_spacing="" hundred_percent_height_center_content="yes" equal_height_columns="no" container_tag="div" menu_anchor="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" status="published" publish_date="" class="" id="" spacing_medium="" margin_top_medium="" margin_bottom_medium="" spacing_small="" margin_top_small="" margin_bottom_small="" margin_top="" margin_bottom="" padding_dimensions_medium="" padding_top_medium="" padding_right_medium="" padding_bottom_medium="" padding_left_medium="" padding_dimensions_small="" padding_top_small="" padding_right_small="" padding_bottom_small="" padding_left_small="" padding_top="" padding_right="" padding_bottom="" padding_left="" link_color="" link_hover_color="" border_sizes="" border_sizes_top="" border_sizes_right="" border_sizes_bottom="" border_sizes_left="" border_color="" border_style="solid" border_radius_top_left="" border_radius_top_right="" border_radius_bottom_right="" border_radius_bottom_left="" box_shadow="no" box_shadow_vertical="" box_shadow_horizontal="" box_shadow_blur="0" box_shadow_spread="0" box_shadow_color="" box_shadow_style="" z_index="" overflow="" gradient_start_color="" gradient_end_color="" gradient_start_position="0" gradient_end_position="100" gradient_type="linear" radial_direction="center center" linear_angle="180" background_color="" background_image="" skip_lazy_load="" background_position="center center" background_repeat="no-repeat" fade="no" background_parallax="none" enable_mobile="no" parallax_speed="0.3" background_blend_mode="none" video_mp4="" video_webm="" video_ogv="" video_url="" video_aspect_ratio="16:9" video_loop="yes" video_mute="yes" video_preview_image="" pattern_bg="none" pattern_custom_bg="" pattern_bg_color="" pattern_bg_style="default" pattern_bg_opacity="100" pattern_bg_size="" pattern_bg_blend_mode="normal" mask_bg="none" mask_custom_bg="" mask_bg_color="" mask_bg_accent_color="" mask_bg_style="default" mask_bg_opacity="100" mask_bg_transform="left" mask_bg_blend_mode="normal" render_logics="" absolute="off" absolute_devices="small,medium,large" sticky="off" sticky_devices="small-visibility,medium-visibility,large-visibility" sticky_background_color="" sticky_height="" sticky_offset="" sticky_transition_offset="0" scroll_offset="0" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset="" filter_hue="0" filter_saturation="100" filter_brightness="100" filter_contrast="100" filter_invert="0" filter_sepia="0" filter_opacity="100" filter_blur="0" filter_hue_hover="0" filter_saturation_hover="100" filter_brightness_hover="100" filter_contrast_hover="100" filter_invert_hover="0" filter_sepia_hover="0" filter_opacity_hover="100" filter_blur_hover="0"][fusion_builder_row][fusion_builder_column type="1_1" layout="1_1" align_self="auto" content_layout="column" align_content="flex-start" valign_content="flex-start" content_wrap="wrap" spacing="" center_content="no" column_tag="div" link="" target="_self" link_description="" min_height="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" sticky_display="normal,sticky" class="" id="" type_medium="" type_small="" order_medium="0" order_small="0" dimension_spacing_medium="" dimension_spacing_small="" dimension_spacing="" dimension_margin_medium="" dimension_margin_small="" margin_top="" margin_bottom="" padding_medium="" padding_small="" padding_top="" padding_right="" padding_bottom="" padding_left="" hover_type="none" border_sizes="" border_color="" border_style="solid" border_radius="" box_shadow="no" dimension_box_shadow="" box_shadow_blur="0" box_shadow_spread="0" box_shadow_color="" box_shadow_style="" z_index_subgroup="regular" z_index="" z_index_hover="" overflow="" background_type="single" gradient_start_color="" gradient_end_color="" gradient_start_position="0" gradient_end_position="100" gradient_type="linear" radial_direction="center center" linear_angle="180" background_color="" background_image="" background_image_id="" lazy_load="none" skip_lazy_load="" background_position="left top" background_repeat="no-repeat" background_blend_mode="none" render_logics="" sticky="off" sticky_devices="small-visibility,medium-visibility,large-visibility" sticky_offset="" absolute="off" absolute_props="" filter_type="regular" filter_hover_element="self" filter_hue="0" filter_saturation="100" filter_brightness="100" filter_contrast="100" filter_invert="0" filter_sepia="0" filter_opacity="100" filter_blur="0" filter_hue_hover="0" filter_saturation_hover="100" filter_brightness_hover="100" filter_contrast_hover="100" filter_invert_hover="0" filter_sepia_hover="0" filter_opacity_hover="100" filter_blur_hover="0" transform_type="regular" transform_hover_element="self" transform_scale_x="1" transform_scale_y="1" transform_translate_x="0" transform_translate_y="0" transform_rotate="0" transform_skew_x="0" transform_skew_y="0" transform_scale_x_hover="1" transform_scale_y_hover="1" transform_translate_x_hover="0" transform_translate_y_hover="0" transform_rotate_hover="0" transform_skew_x_hover="0" transform_skew_y_hover="0" transform_origin="" transition_duration="300" transition_easing="ease" transition_custom_easing="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset="" last="true" border_position="all" first="true"][fusion_text columns="" column_min_width="" column_spacing="" rule_style="" rule_size="" rule_color="" hue="" saturation="" lightness="" alpha="" content_alignment_medium="" content_alignment_small="" content_alignment="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" sticky_display="normal,sticky" class="" id="" margin_top="" margin_right="" margin_bottom="" margin_left="" fusion_font_family_text_font="" fusion_font_variant_text_font="" font_size="" line_height="" letter_spacing="" text_transform="" text_color="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset=""]

    MEM Alpha Modification in Earle’s Buffer (MEM-α)

    Background

    MEM Alpha Modification in Earle’s Buffer (MEM-α) is certainly a suitable nutrient for mammalian cell culture as well as selection for transfected DHFR-negative cells. MEM -α Modification is widely used for primary osteoblast cultures as well as isolation of polymorphonuclear leukocytes (PMN) from umbilical cord blood. Eliminating calcium in this media facilitates the growth of cells in suspension cultures.

    MEM -α  contains

    • Non-essential amino acids.
    • Sodium pyruvate.
    • Lipoic acid.
    • Vitamin B12.
    • Biotin and,
    • Ascorbic acid.
    • Also, MEM-α does not contain deoxyribonucleosides and ribonucleosides.
    • Moreover, PurMaTM MEM -α media in Earle's buffer includes Earles’ buffer

    The composition of Earle's buffer, it contains:

    1. Calcium chloride
    2. Magnesium sulfate
    3. Potassium chloride
    4. Sodium bicarbonate
    5. Sodium chloride
    6. Sodium Phosphate, monobasic

    Additionally, MEM Alpha Modification in Earle’s Buffer (MEM-α)

    it contains:
    • Low level of glucose (1.00 g/ liter)
    • More importantly, Sodium bicarbonate (2.2 g/L) (if higher than 5% CO2 is used, a higher % of sodium bicarbonate is needed.
    • 2 mM L-glutamine (292 mg/L)’
    • Also, PurMa Biologics manufactures 118 types of MEM-α

    Application

    This media can also be used with a variety of suspension and adherent cells including keratinocytes, primary rat astrocytes as well as human melanoma cells. Furthermore, Alpha modification of Minimum Essential Medium Eagle has also been used for the isolation of mesenchymal stem cells, adipose-derived stem cells, and osteoblast.

    Why PurMa Biologics? Why we stand behind our cell culture media.

    Our products are the outcome of 30 years of experience in cutting edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. Moreover, we utilize %99 pure amino acids. This will significantly increase the consistency among your experiments. Considering the highest quality, our prices certainly are extremely reasonable.

    Formulation

    Complete formulation is in the " Formulation tab" as well as here: MEM-alpha Formulation in Earle's Buffer

    References

    1. The growth of L-cells and Vero cells on an autoclavable MEM-peptone medium. Keay et al.  Biotechnology and bioengineering, 19(3), 399-411 (1977-03-01).
    2. Sphingosine-1-phosphate lyase (SGPL1) deficiency is associated with mitochondrial dysfunction. Maharaj et al. The Journal of steroid biochemistry and molecular biology, 202, 105730-105730 (2020-07-20)
    [/fusion_text][fusion_table fusion_table_type="2" fusion_table_rows="13" fusion_table_columns="2" margin_top="30px" margin_right="" margin_bottom="" margin_left="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" class="" id="" animation_type="" animation_direction="left" animation_color="" hue="" saturation="" lightness="" alpha="" animation_speed="0.3" animation_delay="0" animation_offset=""]
    Parameter Specification
    Appearance Red, clear liquid
    pH 7.2 ± 0.1
    Osmolality 275-360 mOsm/L
    Endotoxin NMT< 2EU/mL
    Mycoplasma Negative
    Suitability  Suitable for mammalian cell culture
    Additive Sodium pyruvate
    Indicator Phenol red
    Mycoplasma Detection Negative
    Sterility Tested Sterile filtered using 0.22 µm filter
    Form Liquid
    Shipping Condition  Room temperature
    [/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed Read more
  • MEM Alpha with Nucleosides in Earle’s Buffer (MEM-α-N-EBSS)

    [fusion_builder_container type="flex" hundred_percent="no" hundred_percent_height="no" min_height_medium="" min_height_small="" min_height="" hundr... 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link_hover_color="" border_sizes="" border_sizes_top="" border_sizes_right="" border_sizes_bottom="" border_sizes_left="" border_color="" border_style="solid" border_radius_top_left="" border_radius_top_right="" border_radius_bottom_right="" border_radius_bottom_left="" box_shadow="no" box_shadow_vertical="" box_shadow_horizontal="" box_shadow_blur="0" box_shadow_spread="0" box_shadow_color="" box_shadow_style="" z_index="" overflow="" gradient_start_color="" gradient_end_color="" gradient_start_position="0" gradient_end_position="100" gradient_type="linear" radial_direction="center center" linear_angle="180" background_color="" background_image="" skip_lazy_load="" background_position="center center" background_repeat="no-repeat" fade="no" background_parallax="none" enable_mobile="no" parallax_speed="0.3" background_blend_mode="none" video_mp4="" video_webm="" video_ogv="" video_url="" video_aspect_ratio="16:9" video_loop="yes" video_mute="yes" video_preview_image="" pattern_bg="none" pattern_custom_bg="" pattern_bg_color="" pattern_bg_style="default" pattern_bg_opacity="100" pattern_bg_size="" pattern_bg_blend_mode="normal" mask_bg="none" mask_custom_bg="" mask_bg_color="" mask_bg_accent_color="" mask_bg_style="default" mask_bg_opacity="100" mask_bg_transform="left" mask_bg_blend_mode="normal" render_logics="" absolute="off" absolute_devices="small,medium,large" sticky="off" sticky_devices="small-visibility,medium-visibility,large-visibility" sticky_background_color="" sticky_height="" sticky_offset="" sticky_transition_offset="0" scroll_offset="0" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset="" filter_hue="0" filter_saturation="100" filter_brightness="100" filter_contrast="100" filter_invert="0" filter_sepia="0" filter_opacity="100" filter_blur="0" filter_hue_hover="0" filter_saturation_hover="100" filter_brightness_hover="100" filter_contrast_hover="100" filter_invert_hover="0" filter_sepia_hover="0" filter_opacity_hover="100" filter_blur_hover="0"][fusion_builder_row][fusion_builder_column type="1_1" layout="1_1" align_self="auto" content_layout="column" align_content="flex-start" valign_content="flex-start" content_wrap="wrap" spacing="" center_content="no" column_tag="div" link="" target="_self" link_description="" min_height="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" sticky_display="normal,sticky" class="" id="" type_medium="" type_small="" order_medium="0" order_small="0" dimension_spacing_medium="" dimension_spacing_small="" dimension_spacing="" dimension_margin_medium="" dimension_margin_small="" margin_top="" margin_bottom="" padding_medium="" padding_small="" padding_top="" padding_right="" padding_bottom="" padding_left="" hover_type="none" border_sizes="" border_color="" border_style="solid" border_radius="" box_shadow="no" dimension_box_shadow="" box_shadow_blur="0" box_shadow_spread="0" box_shadow_color="" box_shadow_style="" z_index_subgroup="regular" z_index="" z_index_hover="" overflow="" background_type="single" gradient_start_color="" gradient_end_color="" gradient_start_position="0" gradient_end_position="100" gradient_type="linear" radial_direction="center center" linear_angle="180" background_color="" background_image="" background_image_id="" lazy_load="none" skip_lazy_load="" background_position="left top" background_repeat="no-repeat" background_blend_mode="none" render_logics="" sticky="off" sticky_devices="small-visibility,medium-visibility,large-visibility" sticky_offset="" absolute="off" absolute_props="" filter_type="regular" filter_hover_element="self" filter_hue="0" filter_saturation="100" filter_brightness="100" filter_contrast="100" filter_invert="0" filter_sepia="0" filter_opacity="100" filter_blur="0" filter_hue_hover="0" filter_saturation_hover="100" filter_brightness_hover="100" filter_contrast_hover="100" filter_invert_hover="0" filter_sepia_hover="0" filter_opacity_hover="100" filter_blur_hover="0" transform_type="regular" transform_hover_element="self" transform_scale_x="1" transform_scale_y="1" transform_translate_x="0" transform_translate_y="0" transform_rotate="0" transform_skew_x="0" transform_skew_y="0" transform_scale_x_hover="1" transform_scale_y_hover="1" transform_translate_x_hover="0" transform_translate_y_hover="0" transform_rotate_hover="0" transform_skew_x_hover="0" transform_skew_y_hover="0" transform_origin="" transition_duration="300" transition_easing="ease" transition_custom_easing="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset="" last="true" border_position="all" first="true"][fusion_text columns="" column_min_width="" column_spacing="" rule_style="" rule_size="" rule_color="" hue="" saturation="" lightness="" alpha="" content_alignment_medium="" content_alignment_small="" content_alignment="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" sticky_display="normal,sticky" class="" id="" margin_top="" margin_right="" margin_bottom="" margin_left="" fusion_font_family_text_font="" fusion_font_variant_text_font="" font_size="" line_height="" letter_spacing="" text_transform="" text_color="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset=""]

    MEM Alpha with Nucleosides in Earle’s Buffer (MEM-α-N-EBSS)

    Background

    MEM Alpha with Nucleosides in Earle’s Buffer (MEM-α-N-EBSS) is a suitable nutrient for mammalian cell culture as well as selection for transfected DHFR-negative cells. This media contains nucleoside, and the formulation is available below. depending on cell types, laboratories use MEM-α-N for growing mammalian cell for a variety of suspension as well as adherent cells. Eliminating calcium in this media facilitates the growth of cells in suspension cultures.

    Ingredients

    MEM Alpha Modification with Nucleosides in Earle’s Buffer (MEM-α-N-EBSS) is a modification of MEM.

    MEM-α-N-EBSS contains:

    • Non-essential amino acids.
    • Sodium pyruvate.
    • Lipoic acid.
    • Vitamin B12.
    • Biotin and,
    • Ascorbic acid.

    PurMa™ MEM-α-N-EBSS includes Earles’ Buffer

    As the composition of Earls buffer, it contains:
    1. Calcium chloride
    2. Magnesium sulfate
    3. Potassium chloride
    4. Sodium bicarbonate
    5. Sodium chloride
    6. Sodium Phosphate, monobasic

    PurMa™ MEM -α-N-EBSS media also contains:

    • Low level of glucose (1.00 g/ liter)
    • Sodium bicarbonate (2.2 g/L) (if higher than 5% CO2 is used, a higher % of sodium bicarbonate is needed.
    • 2 mM L-glutamine (292 mg/L)’
    • PurMa Biologics manufactures 121 types of MEM -α-N-E

    The nucleoside modifies this media  from MEM-α

    it contains:
    • Ribonucleosides
    • Deoxyribonucleosides

    Why PurMa Biologics? Why we stand behind our cell culture media

    Our products are the outcome of 30 years of experience in cutting edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. Moreover, We utilize %99 pure amino acids. This will significantly increase the consistency among your experiments. Considering the highest quality, our prices certainly are extremely reasonable.

    Application

    This media can also be used for keratinocytes, primary rat astrocytes, as well as human melanoma cells. MEM-α-N-EBSS has also been used for the isolation of mesenchymal stem cells, adipose-derived stem cells, and osteoblast. Additionally, PurMa™ MEM -α-N-EBSS facilitates the growth and maintenance of primary osteoblast cultures. furthermore, this media performs well for isolation of polymorphonuclear leukocytes (PMN) from umbilical cord blood.

    Formulation

    Click here for complete formulation as well as in " Formulation Tab": MEM alpha with Nucleoside Formulation

    References

    1. The growth of L-cells and Vero cells on an autoclavable MEM-peptone medium. Keay, L. Biotechnology and bioengineering, 19(3), 399-411 (1977-03-01).
    2. Sphingosine-1-phosphate lyase (SGPL1) deficiency is associated with mitochondrial dysfunction.  Maharaj et al. The Journal of steroid biochemistry and molecular biology, 202, 105730-105730 (2020-07-20)
    3. Minimum essential medium alpha (MEM) enhances assisted reproductive technology results. I. Mouse embryo study. Wun et al. J Assist Reprod Genet. 1994 Jul;11(6):303-7. doi: 10.1007/BF02215717. PMID: 7734915.
    [/fusion_text][fusion_table fusion_table_type="2" fusion_table_rows="13" fusion_table_columns="2" margin_top="30px" margin_right="" margin_bottom="" margin_left="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" class="" id="" animation_type="" animation_direction="left" animation_color="" hue="" saturation="" lightness="" alpha="" animation_speed="0.3" animation_delay="0" animation_offset=""]
    Parameter Specification
    Appearance Red, clear liquid
    pH 7.2 ± 0.1
    Osmolality 275-360 mOsm/L
    Endotoxin NMT< 2EU/mL
    Mycoplasma Negative
    Suitability  Suitable for mammalian cell culture
    Additive Sodium pyruvate
    Indicator Phenol red
    Mycoplasma Detection Negative
    Sterility Tested Sterile filtered using 0.22 µm filter
    Form Liquid
    Shipping Condition  Room temperature
    [/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed Read more
  • MEM α-Modification with Nucleosides in Hanks’ Buffer

    [fusion_builder_container type="flex" hundred_percent="no" hundred_percent_height="no" min_height_medium="" min_height_small="" min_height="" hundr... 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link_hover_color="" border_sizes="" border_sizes_top="" border_sizes_right="" border_sizes_bottom="" border_sizes_left="" border_color="" border_style="solid" border_radius_top_left="" border_radius_top_right="" border_radius_bottom_right="" border_radius_bottom_left="" box_shadow="no" box_shadow_vertical="" box_shadow_horizontal="" box_shadow_blur="0" box_shadow_spread="0" box_shadow_color="" box_shadow_style="" z_index="" overflow="" gradient_start_color="" gradient_end_color="" gradient_start_position="0" gradient_end_position="100" gradient_type="linear" radial_direction="center center" linear_angle="180" background_color="" background_image="" skip_lazy_load="" background_position="center center" background_repeat="no-repeat" fade="no" background_parallax="none" enable_mobile="no" parallax_speed="0.3" background_blend_mode="none" video_mp4="" video_webm="" video_ogv="" video_url="" video_aspect_ratio="16:9" video_loop="yes" video_mute="yes" video_preview_image="" pattern_bg="none" 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    MEM α-Modification with Nucleosides in Hanks’ Buffer

    Background

    MEM α-Modification with Nucleosides  in Hanks’ buffer provides a great nutritional culture environment during mammalian cells.  This media, as well plays a key role in the process of selection of transfected DHFR-negative cells. Moreover, MEM-α-N-HBSS is widely used for a variety of suspension and adherent cells. Calcium, however, plays a crucial role in media as eliminating this ion facilitates the growth of cells in suspension cultures.

    Ingredients

    MEM-α with nucleosides is basically the modified version of MEM-α and contains:
    • Ribonucleosides
    • Deoxyribonucleosides
    • Non-essential amino acids.
    • Sodium pyruvate.
    • Lipoic acid.
    • Vitamin B12.
    • Biotin and,
    • Ascorbic acid.

    PurMa™ MEM-α-N-HBSS contains HBSS buffer

    The composition of HBSS buffer:
    1. Potassium Chloride
    2. Potassium Phosphate, monobasic
    3. Sodium Bicarbonate
    4. Sodium Chloride
    5. Sodium Phosphate, dibasic
    Additionally, MEM-α-N-HBSS also Contains:
    • Low level of glucose (1.00 g/ liter)
    • Sodium bicarbonate (0.35 g/L)
    • 2 mM L-glutamine (292 mg/L)
    PurMa Biologics manufactures 119 types of MEM-α-N-HBSS

    Why PurMa Biologics? Why we stand behind our cell culture media

    Our products are the outcome of 30 years of experience in cutting-edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. Moreover, We utilize %99 pure amino acids. This will significantly increase the consistency among your experiments. Considering the highest quality, our prices certainly are extremely reasonable.

    Application

    Furthermore,  MEM-α-N-HBSS is used for various cell types such as keratinocytes, primary rat astrocytes, as well as human melanoma cells. also, MEM-α-N-HBSS has been used for the isolation of mesenchymal stem cells, adipose-derived stem cells, and osteoblasts. Moreover, MEM α-Modification is widely used for primary osteoblast cultures.

    Formulation

    For complete formulation  click here as well as " formulation Tab : MEM alpha with nucleoside in HBSS Buffer Formulation

    References

    1. A previously functional tetracycline-regulated transactivator fails to target gene expression to the bone. Schmidt et al. BMC Res Notes. 2011 Aug 11;4:282. doi: 10.1186/1756-0500-4-282. PMID: 21835026.
    2. The growth of L-cells and Vero cells on an autoclavable MEM-peptone medium.  Keay L, Biotechnology and bioengineering, 19(3), 399-411 (1977-03-01
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    Parameter Specification
    Appearance Red, clear liquid
    pH 7.2 ± 0.1
    Osmolality 275-360 mOsm/L
    Endotoxin NMT< 2EU/mL
    Mycoplasma Negative
    Suitability  Suitable for mammalian cell culture
    Additive Sodium pyruvate
    Indicator Phenol red
    Mycoplasma Detection Negative
    Sterility Tested Sterile filtered using 0.22 µm filter
    Form Liquid
    Shipping Condition  Room temperature
    [/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed Read more

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