Minimum Essential Media (MEM) in Earle’s Buffer
Background
Minimum Essential Media (MEM) in Earle’s Buffer is a modification of Basal Medium Eagle (BME) which was originally developed by Harry Eagle. It also includes higher concentrations of amino acids for cultured mammalian cells. Moreover, MEM is available with Earle’s salts for use in a CO2 incubator, or with Hanks’ salts for use without CO2.
PurMa™ MEM media includes Earle’s Buffer
As the composition of Earle’s buffer, it contains:
- Calcium chloride
- Magnesium sulfate
- Potassium chloride
- Sodium bicarbonate
- Sodium chloride
- Sodium Phosphate, monobasic
PurMa Biologics manufactures 118 types of MEM, it also includes:
- Low level of glucose (1.00 g/ liter)
- Sodium bicarbonate (2.2 g/L)
- 2 mM L-glutamine (292 mg/L)
MEM has been used for cell lines such as HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts as well as primary rat astrocytes. Additionally, MEM in Earle’s buffer uses a sodium bicarbonate buffer system (2.2 g/L) that requires a 5–10% CO2 environment to maintain physiological pH.
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- Minimum Essential Media (MEM) in Earle’s Buffer, Cell Culture Media
Advanced Minimum Essential Media (A-MEM) in EBSS
- Advanced Minimum Essential Media (A-MEM) in Earle’s Buffer contains L-Alanyl-L-Glutamine as well as ingredients to allow for serum reduction. Additionally, Advanced MEM needs 2-5% FBS and provides more consistency.
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- Minimum Essential Media (MEM) in Earle’s Buffer, Cell Culture Media
Minimum Essential Media (MEM) in Earle’s Buffer
- Minimum Essential Media (MEM) in Earle’s Buffer was originally developed by Harry Eagle. MEM is available with Earle’s salts for use in a CO2 incubator, or with Hanks' salts for use without CO2.
- Read more
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- Minimum Essential Media (MEM) in Earle’s Buffer, Cell Culture Media
Minimum Essential Media MAX (MEM-MAX) in Earle’s Buffer
- Minimum Essential Media MAX (MEM-MAX) in Earle’s Buffer contains L-Alanyl-L-Glutamine, therefore supplying a longer shelf life. MEM-MAX in EBBS significantly improves cell viability and growth. We manufacture several types of MEM-MAX based on your requirements.
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