Minimum Essential Media (MEM) in Earle’s Buffer
Background
Minimum Essential Media (MEM) in Earle’s Buffer is a modification of Basal Medium Eagle (BME) which was originally developed by Harry Eagle. It also includes higher concentrations of amino acids for cultured mammalian cells. Moreover, MEM is available with Earle’s salts for use in a CO2 incubator, or with Hanks’ salts for use without CO2.
PurMa™ MEM media includes Earle’s Buffer
As the composition of Earle’s buffer, it contains:
- Calcium chloride
- Magnesium sulfate
- Potassium chloride
- Sodium bicarbonate
- Sodium chloride
- Sodium Phosphate, monobasic
PurMa Biologics manufactures 118 types of MEM, it also includes:
- Low level of glucose (1.00 g/ liter)
- Sodium bicarbonate (2.2 g/L)
- 2 mM L-glutamine (292 mg/L)
MEM has been used for cell lines such as HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts as well as primary rat astrocytes. Additionally, MEM in Earle’s buffer uses a sodium bicarbonate buffer system (2.2 g/L) that requires a 5–10% CO2 environment to maintain physiological pH.
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- Cell Culture Media, Minimum Essential Media (MEM) in Earle’s Buffer
Chemically Defined Minimum Essential Media in EBSS (CD-MEM/ EBSS)
- PurMa™ Chemically Defined CD MEM in EBSS (CD-MEM/EBSS Medium) does not need any serum, fully synthetic and is free of any trace of animal products. Additionally, the cell growth with CD-MEM/EBSS Medium is equivalent to Medium +10% FBS
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- Cell Culture Media, Minimum Essential Media (MEM) in Earle’s Buffer
Minimum Essential Media (MEM) in Earle’s Buffer
- Price range: $19.11 through $416.15
- Minimum Essential Media (MEM) in Earle’s Buffer was originally developed by Harry Eagle. MEM is available with Earle’s salts for use in a CO2 incubator, or with Hanks' salts for use without CO2.
- Select options This product has multiple variants. The options may be chosen on the product page
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- Sale!
- Cell Culture Media, Minimum Essential Media (MEM) in Earle’s Buffer
Minimum Essential Media MAX (MEM-MAX) in Earle’s Buffer
- Price range: $30.32 through $586.18
- Minimum Essential Media MAX (MEM-MAX) in Earle’s Buffer contains L-Alanyl-L-Glutamine, therefore supplying a longer shelf life. MEM-MAX in EBBS significantly improves cell viability and growth. We manufacture several types of MEM-MAX based on your requirements.
- Select options This product has multiple variants. The options may be chosen on the product page
