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SKU: Select options below to see it Categories: Cell Culture Media, MEM α-Modification with Nucleosides in Hanks’ Buffer Tags: Cell Culture, Hanks’ Buffer, HBSS, MEM Alpha, MEM-α, Nucleosides, Phenol Red, Standard

MEM-Alpha-Modification with Nucleosides in Hanks’ Buffer

MEM α-Modification with Nucleosides in Hanks’ buffer provides a great nutritional culture environment for mammalian cells.  this media as well plays a key role in the process of selection for transfected DHFR-negative cells.

SKU Select options below to see it
SKU: N/A Categories: Cell Culture Media, MEM α-Modification with Nucleosides in Hanks’ Buffer Tags: Cell Culture, Hanks’ Buffer, HBSS, MEM Alpha, MEM-α, Nucleosides, Phenol Red, Standard

Description

Background

MEM α-Modification with Nucleosides  in Hanks’ buffer provides a great nutritional culture environment for mammalian cells.  This media also plays a key role in the process of selection of transfected DHFR-negative cells. Moreover, MEM-α-N-HBSS is widely used for a variety of suspension and adherent cells. Calcium, however, plays a crucial role in media as eliminating this ion facilitates the growth of cells in suspension cultures.

Ingredients

MEM-α with nucleosides is basically the modified version of MEM-α and contains:

  • Ribonucleosides
  • Deoxyribonucleosides
  • Non-essential amino acids.
  • Sodium pyruvate.
  • Lipoic acid.
  • Vitamin B12.
  • Biotin and,
  • Ascorbic acid.

PurMa™ MEM-α-N-HBSS contains HBSS buffer

The composition of HBSS buffer:

  1. Potassium Chloride
  2. Potassium Phosphate, monobasic
  3. Sodium Bicarbonate
  4. Sodium Chloride
  5. Sodium Phosphate, dibasic

Additionally, MEM α-Modification with Nucleosides in Hanks’ Buffer also Contains:

  • Low level of glucose (1.00 g/ liter)
  • Sodium bicarbonate (0.35 g/L)
  • 2 mM L-glutamine (292 mg/L)

PurMa Biologics manufactures 119 types of MEM-α-N-HBSS

Why PurMa Biologics? Why we stand behind our cell culture media

Our products are the outcome of 30 years of experience in cutting-edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. Moreover, We utilize 99% pure amino acids. This will significantly increase the consistency among your experiments. Considering the highest quality, our prices certainly are extremely reasonable.

Application

Furthermore,  MEM-α-N-HBSS is used for various cell types such as keratinocytes, primary rat astrocytes, as well as human melanoma cells. Also, MEM-α-N-HBSS has been used for the isolation of mesenchymal stem cells, adipose-derived stem cells, and osteoblasts. Moreover, MEM α-Modification is widely used for primary osteoblast cultures.

Formulation

For complete formulation  click here as well as ” formulation Tab : MEM alpha with nucleoside in HBSS Buffer Formulation

References

  1. A previously functional tetracycline-regulated transactivator fails to target gene expression to the bone. Schmidt et al. BMC Res Notes. 2011 Aug 11;4:282. doi: 10.1186/1756-0500-4-282. PMID: 21835026.
  2. The growth of L-cells and Vero cells on an autoclavable MEM-peptone medium.  Keay L, Biotechnology and bioengineering, 19(3), 399-411 (1977-03-01
Parameter Specification
Appearance Red, clear liquid
pH 7.2 ± 0.1
Osmolality 275-360 mOsm/L
Endotoxin NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive Sodium pyruvate
Indicator Phenol red
Mycoplasma Detection Negative
Sterility Tested Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

 

Additional information

Condition

MEM-α-N (HBSS) Standard Formulation, MEM-α-N (HBSS) w/o Glutamine, MEM-α-N (HBSS) w/o Phenol Red, MEM-α-N (HBSS) w/o Sodium Bicarbonate, MEM-α-N (HBSS) w/o Glucose, MEM-α-N (HBSS) w/o Lipoic Acid, MEM-α-N (HBSS) w/o Glutamine, w/o Phenol Red, MEM-α-N (HBSS) w/o Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, MEM-α-N (HBSS) 15 mM HEPES (3.6 g/L), MEM-α-N (HBSS) 25 mM HEPES (5.9 g/L), MEM-α-N (HBSS) w/o Sodium Pyruvate, MEM-α-N (HBSS) High Sodium Bicarbonate (0.85 g/L), MEM-α-N (HBSS) High Glucose (4.5 g/L), MEM-α-N (HBSS) 15 mM HEPES (3.6 g/L), High Glucose (4.5 g/L), MEM-α-N (HBSS) 25 mM HEPES (5.9 g/L), High Glucose (4.5 g/L)
Format

Liquid, Powder
Size

10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L

Documents

Certificate of Analysis (COA): Available upon request for your specific order — email info@purmabiologics.com and we will send the current lot documentation.

Safety Data Sheet (SDS): Available upon request — email info@purmabiologics.com.

What our customers say about PurMa Biologics

Feedback from labs we supply, across our full catalogue.

★★★★★
Very fast, efficient, and economic service. Great for new PIs like me.
Surendra Shukla OU Health Sciences Center
★★★★★
it is reliable vendor!
Jianhua Song Applied StemCell
★★★★★
We always buy our agarose from PurMa and have never been disappointed.
Zhenghui Liu Applied StemCell
★★★★★
I recently had the pleasure of interacting with Dr. Seif Azadi from Purma Biologics, and I cannot thank him enough for his expertise and prompt assistance. His deep knowledge and dedication were instrumental in meeting our laboratory’s needs with remarkable efficiency. We ordered FBS, DMEM, and Trypsin from Purma Biologics, and I’m thrilled to report that all the products have performed exceptionally well. They consistently deliver reproducible results, significantly enhancing the reliability of our experiments. Thank you, Team Purma Biologics, for your good products and support!
Ravi Gor University of Oklahoma
★★★★★
It’s been a pleasure working with PURMA Biologics. Their service is efficient, delivery is fast, and the quality of their products has met our expectations consistently. Very smooth collaboration overall.
Maya Hart Tak Biomed
★★★★★
PurMa products are of excellent quality and highly reliable.
East Tennessee State University Verified customer

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Frequently asked questions

How should I store MEM-Alpha-Modification with Nucleosides in Hanks’ Buffer?

Store at 2 to 8 degrees C, protected from light, and do not freeze unless the label states otherwise. Freezing can precipitate salts and degrade light sensitive components such as riboflavin and folic acid. Always follow the storage line on the Certificate of Analysis for the specific lot.

What is the shelf life once opened?

Unopened and correctly stored, the typical shelf life is 12 months from manufacture. Once opened, use within about four weeks and always handle in a laminar flow hood, since the practical limit after opening is contamination risk rather than chemical stability.

Is it sterility and endotoxin tested?

Yes. Every lot is sterile filtered and tested for sterility and endotoxin, and a Certificate of Analysis is issued for each lot. The COA is available on request for any catalogue number.

Do I need to add L-glutamine?

It depends on the formulation you order. Standard formulations may contain free L-glutamine, which degrades in solution over time, while MAX formulations use a stable L-alanyl-L-glutamine dipeptide that does not require fresh supplementation. Check the option you have selected before adding more.

Can I order this in bulk or as a custom formulation?

Yes. Bulk volumes and custom formulations are made to order, including adjusted glucose, bicarbonate, buffering and cation content. Use the request bulk pricing button on this page and include the specification you need.

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