MEM α-Modification with Nucleosides in Hanks’ buffer provides a great nutritional culture environment for mammalian cells. this media as well plays a key role in the process of selection for transfected DHFR-negative cells.
Minimum Essential Media (MEM) in Earle’s Buffer was originally developed by Harry Eagle. MEM is available with Earle’s salts for use in a CO2 incubator, or with Hanks' salts for use without CO2.
Minimum Essential Media (MEM) In Hanks’ Buffer is a modification of Basal Medium Eagle. This media includes higher concentrations of amino acids making it suitable for cultured mammalian cells. Moreover, PurMa Biologics manufactures MEM with Earle’s buffer for use in a CO2 incubator, or with Hanks’ salts for use without CO2.
Roswell Park Memorial Institute 1640 (RPMI-1640) are being used for culturing human normal and neoplastic leukocytes, HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, carcinomas, bone marrow, as well as hybridoma cells.
Dulbecco’s Modified Eagle’s Medium/F-12 (DMEM F-12) is commonly used worldwide. Moreover, DMEM F-12 contains more nutrition and offers more buffering capacity. Therefore, this medium provides more growth and maintenance ability.
Williams′ Medium E has been used for primary and also commercial cell lines including: primary liver epithelial cells, HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes ,carcinomas as well as hybridoma cells.