Basal Medium Eagle (BME) in Hanks’ Buffer was originally developed by Harry Eagle. Moreover, this media is one of the most widely used of all synthetic cell culture media. Furthermore, BME is the predecessor of Eagle’s Minimum Essential Medium (MEM) and Dulbecco’s Modified Eagle’s Medium (DME).
Dulbecco’s Modified Eagle’s Medium is a modification of Basal Medium Eagle. Our DMEM, optimized to deliver exceptional growth and consistency, is available in Standard, MAX, and Advanced formulations. Furthermore, PurMa Biologics currently manufactures 145 conditions of this media.
Dulbecco’s Modified Eagle’s Medium/F-12 (DMEM F-12) is commonly used worldwide. Moreover, DMEM F-12 contains more nutrition and offers more buffering capacity. Therefore, this medium provides more growth and maintenance ability.
Eagle's Minimal Essential Medium (EMEM) is suitable for adhesive as well as suspension cell lines. PurMa Biologics manufactures 106 types of this medium
Glasgow’s Modified Eagle’s Medium was originally developed to study the polyoma virus. However, due to particular criteria has been mentioned in the long description, this media is used without serum.
Although, Iscove's Modified Dulbecco's Medium (IMDM) is specialized for rapidly proliferating, cell lines, it certainly quite suitable for slow growing cells as well. Moreover, this medium is the only medium that lacks Iron, with potassium nitrate replacing ferric nitrate , therefore makes it an excellent choice for adhesive cells.
Leibovitz's L-15 Medium was originally designed to be used in carbon dioxide (CO2) free systems. Therefore, does not need sodium bicarbonate to provide the buffering capacity. Instead, phosphates, free base amino acids, salts, and galactose maintain the physiological pH.
Leibovitz's L-15 Medium was originally designed to be used in carbon dioxide (CO2) free systems. Therefore, does not need sodium bicarbonate to provide the buffering capacity. Instead, phosphates, free base amino acids, salts, and galactose maintain the physiological pH.
McCoy’s 5A Modified Media (M5AMM) is widely used cor tumor cells. Additionally, modified version of this media contains several folds of amino acids and vitamins compared to the original formulation.
MEM Alpha Modification (MEM-α) in Hanks’ Buffer is suitable for mammalian cell culture . Moreover, This media can also be used with a variety of suspension as well as adherent cells including keratinocytes, primary rat astrocytes, and human melanoma cells.
MEM Alpha Modification in Earle’s Buffer (MEM-α) is a suitable nutrient for mammalian cell culture as well as selection for transfected DHFR-negative cells. Scientist around the globe use MEM -α Modification for primary osteoblast cultures as well as isolation of polymorphonuclear leukocytes (PMN)
MEM Alpha with Nucleosides in Earle’s Buffer (MEM-α-N-EBSS) is a suitable nutrient for mammalian cell culture as well as selection for transfected DHFR-negative cells. This media contains nucleoside and, furthermore depending on cell types, laboratories use MEM-α-N for growing mammalian cells.
MEM α-Modification with Nucleosides in Hanks’ buffer provides a great nutritional culture environment for mammalian cells. this media as well plays a key role in the process of selection for transfected DHFR-negative cells.
Minimum Essential Media (MEM) in Earle’s Buffer was originally developed by Harry Eagle. MEM is available with Earle’s salts for use in a CO2 incubator, or with Hanks' salts for use without CO2.
Minimum Essential Media (MEM) In Hanks’ Buffer is a modification of Basal Medium Eagle. This media includes higher concentrations of amino acids making it suitable for cultured mammalian cells. Moreover, PurMa Biologics manufactures MEM with Earle’s buffer for use in a CO2 incubator, or with Hanks’ salts for use without CO2.
Roswell Park Memorial Institute 1640 (RPMI-1640) are being used for culturing human normal and neoplastic leukocytes, HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, carcinomas, bone marrow, as well as hybridoma cells.
Dulbecco’s Modified Eagle’s Medium/F-12 (DMEM F-12) is commonly used worldwide. Moreover, DMEM F-12 contains more nutrition and offers more buffering capacity. Therefore, this medium provides more growth and maintenance ability.
Williams′ Medium E has been used for primary and also commercial cell lines including: primary liver epithelial cells, HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes ,carcinomas as well as hybridoma cells.