MEM Alpha Modification (MEM-α) in Hanks’ Buffer
Background
MEM Alpha Modification (MEM-α) in Hanks’ Buffer is certainly a suitable choice for mammalian cell culture as well as selection for transfected DHFR-negative cells. Besides, this media can also be used with a variety of suspension and adherent cells including keratinocytes, primary rat astrocytes, and human melanoma cells. Furthermore, it is important that eliminating calcium in this media facilitates the growth of cells in suspension cultures.
Ingredients
MEM -α has improved regular MEM as it contains:
- Non-essential amino acids.
- Sodium pyruvate.
- Lipoic acid.
- Vitamin B12.
- Biotin and,
- Ascorbic acid.
- Also, MEM-α does not contain deoxyribonucleosides and ribonucleosides.
Moreover, PurMa™ MEM -α media in Hank’s buffer includes HBSS
Composition of HBSS buffer contains:
- Potassium Chloride
- Potassium Phosphate, monobasic
- Sodium Bicarbonate
- Sodium Chloride
- Sodium Phosphate, dibasic
- Low level of glucose (1.00 g/ liter)
- Sodium bicarbonate (0.35 g/L)
- 2 mM L-glutamine (292 mg/L)
- Low level of glucose (1.00 g/ liter)
Furthermore, PurMa Biologics manufactures 123 types of MEM with HBSS buffer
Application
Alpha modification of Minimum Essential Medium Eagle certainly makes an excellent for the isolation of mesenchymal stem cells, adipose-derived stem cells as well as osteoblast. MEM -α Modification is widely used for primary osteoblast cultures as well as isolation of polymorphonuclear leukocytes (PMN) from umbilical cord blood.
Formulation
additionally, to get access to detailed formulation visit ” formulation Tab” as well as by clicking here : MEM-alpha Formulation in Hank’s Buffer (HBSS)
References
- The growth of L-cells and Vero cells on an autoclavable MEM-peptone medium. Keay L. Biotechnology and bioengineering, 19(3), 399-411 (1977-03-01)
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- Sale!
- MEM Alpha Modification (MEM-α) in Hanks’ Buffer, Cell Culture Media
Advanced MEM Alpha-Modification (A-MEM-α) in Hanks’ Buffer
- $53.99 – $1125.68
- Advanced MEM Alpha-Modification (A-MEM-α) in Hanks’ Buffer contains L-Alanyl-L-Glutamine as well as ingredients to allow for serum reduction. Additionally, Advanced MEM-α needs 2-5% FBS and provides more consistency.
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- Sale!
- MEM Alpha Modification (MEM-α) in Hanks’ Buffer
MEM Alpha Modification (MEM-α) in Hanks’ Buffer
- $48.05 – $1092.12
- MEM Alpha Modification (MEM-α) in Hanks’ Buffer is suitable for mammalian cell culture . Moreover, This media can also be used with a variety of suspension as well as adherent cells including keratinocytes, primary rat astrocytes, and human melanoma cells.
- Select options
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- Sale!
- MEM Alpha Modification (MEM-α) in Hanks’ Buffer, Cell Culture Media
MEM Alpha-Modification MAX (MEM-α-MAX) in Hanks’ Buffer
- $50.99 – $1109.66
- MEM Alpha-Modification MAX (MEM-α-MAX) in Hanks’ Buffer contains L-Alanyl-L-Glutamine, therefore supplying a longer shelf life. MEM-α-MAX in HBBS significantly improves cell viability and growth. We manufacture several types of MEM-α-MAX based on your requirements.
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