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MEM Alpha Modification (MEM-α) in Hanks’ Buffer
MEM Alpha Modification (MEM-α) in Hanks’ Buffer is suitable for mammalian cell culture . Moreover, This media can also be used with a variety of suspension as well as adherent cells including keratinocytes, primary rat astrocytes, and human melanoma cells.
Description
Background
MEM Alpha Modification (MEM-α) in Hanks’ Buffer is certainly a suitable choice for mammalian cell culture as well as selection for transfected DHFR-negative cells. This media can also be used with a variety of suspension and adherent cells including keratinocytes, primary rat astrocytes, and human melanoma cells. Furthermore, eliminating calcium in this media facilitates the growth of cells in suspension cultures.
Ingredients
MEM -α has improved regular MEM as it contains:
- Non-essential amino acids.
- Sodium pyruvate.
- Lipoic acid.
- Vitamin B12.
- Biotin and,
- Ascorbic acid.
- Also, MEM-α does not contain deoxyribonucleosides and ribonucleosides.
PurMa™ MEM -α media in Hank’s buffer includes HBSS
Composition of HBSS buffer contains:
- Potassium Chloride
- Potassium Phosphate, monobasic
- Sodium Bicarbonate
- Sodium Chloride
- Sodium Phosphate, dibasic
- Low level of glucose (1.00 g/ liter)
- Sodium bicarbonate (0.35 g/L)
- 2 mM L-glutamine (292 mg/L)
- Low level of glucose (1.00 g/ liter)
Furthermore, PurMa Biologics manufactures 123 types of MEM with HBSS buffer
Why PurMa Biologics? Why we stand behind our cell culture media
Our products are the outcome of 30 years of experience in cutting edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. Moreover, We utilize %99 pure amino acids. This will significantly increase the consistency among your experiments. Considering the highest quality, our prices certainly are extremely reasonable.
Application
Alpha modification of Minimum Essential Medium Eagle certainly makes an excellent choice for the isolation of mesenchymal stem cells, adipose-derived stem cells as well as osteoblast. MEM -α Modification is widely used for primary osteoblast cultures as well as isolation of polymorphonuclear leukocytes (PMN) from umbilical cord blood.
Formulation
to get access to detailed formulation visit ” formulation Tab” as well as by clicking here : MEM-alpha Formulation in Hank’s Buffer (HBSS)
References
- The growth of L-cells and Vero cells on an autoclavable MEM-peptone medium. Keay L. Biotechnology and bioengineering, 19(3), 399-411 (1977-03-01).
- Sphingosine-1-phosphate lyase (SGPL1) deficiency is associated with mitochondrial dysfunction. Maharaj et al. The Journal of steroid biochemistry and molecular biology, 202, 105730-105730 (2020-07-20)
| Parameter | Specification |
|---|---|
| Appearance | Red, clear liquid |
| pH | 7.2 ± 0.1 |
| Osmolality | 275-360 mOsm/L |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Suitability | Suitable for mammalian cell culture |
| Additive | Sodium pyruvate |
| Indicator | Phenol red |
| Mycoplasma Detection | Negative |
| Sterility Tested | Sterile filtered using 0.22 µm filter |
| Form | Liquid |
| Shipping Condition | Room temperature |
Additional information
| Condition | MEM-α (HBSS) Standard Formulation, MEM-α (HBSS) w/o Glutamine, MEM-α (HBSS) w/o Phenol Red, MEM-α (HBSS) w/o Sodium Bicarbonate, MEM-α (HBSS) w/o Glucose, MEM-α (HBSS) w/o Lipoic Acid, MEM-α (HBSS) w/o Glutamine, w/o Phenol Red, MEM-α (HBSS) w/o Glutamine, w/o Sodium Bicarbonate, MEM-α (HBSS) w/o Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, MEM-α (HBSS) 15 mM HEPES (3.6 g/L), MEM-α (HBSS) 25 mM HEPES (5.9 g/L), MEM-α (HBSS) w/o Sodium Pyruvate, MEM-α (HBSS) High Glucose (4.5 g/L), MEM-α (HBSS) High Sodium Bicarbonate (0.85 g/L), MEM-α (HBSS) 15 mM HEPES (3.6 g/L), High Glucose (4.5 g/L), MEM-α (HBSS) 25 mM HEPES (5.9 g/L), High Glucose (4.5 g/L) |
|---|---|
| Format | Liquid, Powder |
| Size | 10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L |
Documents
Certificate of Analysis (COA): Available upon request for your specific order — email info@purmabiologics.com and we will send the current lot documentation.
Safety Data Sheet (SDS): Available upon request — email info@purmabiologics.com.
What our customers say about PurMa Biologics
Feedback from labs we supply, across our full catalogue.
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I recently had the pleasure of interacting with Dr. Seif Azadi from Purma Biologics, and I cannot thank him enough for his expertise and prompt assistance. His deep knowledge and dedication were instrumental in meeting our laboratory’s needs with remarkable efficiency. We ordered FBS, DMEM, and Trypsin from Purma Biologics, and I’m thrilled to report that all the products have performed exceptionally well. They consistently deliver reproducible results, significantly enhancing the reliability of our experiments. Thank you, Team Purma Biologics, for your good products and support!
It’s been a pleasure working with PURMA Biologics. Their service is efficient, delivery is fast, and the quality of their products has met our expectations consistently. Very smooth collaboration overall.
PurMa products are of excellent quality and highly reliable.
I've ordered cell culture media multiple times. Great prices and excellent customer service. The ordering process was smooth, and everything arrived as expected. I'll definitely be purchasing from them again.
We buy all of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
Frequently asked questions
How should MEM Alpha Modification (MEM-α) in Hanks’ Buffer be stored?
Store at 2 to 8 degrees C, protected from light, and do not freeze unless the label states otherwise. Freezing can precipitate salts and degrade light sensitive components such as riboflavin and folic acid. Follow the storage line on the Certificate of Analysis for the specific lot.
What is the shelf life once opened?
Unopened and correctly stored, the typical shelf life is 12 months from manufacture. Once opened, use within about four weeks and handle in a laminar flow hood, since the practical limit after opening is contamination risk rather than chemical stability.
Is it sterility and endotoxin tested?
Yes. Every lot is sterile filtered and tested for sterility and endotoxin, and a Certificate of Analysis is issued for each lot and available on request.
Do I need to add L-glutamine?
It depends on the formulation ordered. Standard formulations may contain free L-glutamine, which degrades in solution over time, while MAX formulations use a stable L-alanyl-L-glutamine dipeptide that needs no fresh supplementation. Check the option selected before adding more.
Can I order this in bulk or as a custom formulation?
Yes. Bulk volumes and custom formulations are made to order, including adjusted glucose, bicarbonate, buffering and cation content. Use the request bulk pricing button on this page and include the specification you need.
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