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Minimum Essential Media (MEM) In Hanks’ Buffer

Background

Minimum Essential Media (MEM) In Hanks’ Buffer is a modification of Basal Medium Eagle . It was originally developed by Harry Eagle for specific  requirements of certain subtypes. Additionally, it includes higher concentrations of amino acids which is more suitable for cultured mammalian cells. Moreover, PurMa Biologics manufactures MEM with Earle’s buffer for use in a CO₂ incubator, or with Hanks' salts for use without CO₂.

PurMa^™ MEM media in Hank’s buffer includes Hank's buffer

Hanks buffer, it contains:

1. Potassium Chloride
2. Potassium Phosphate, monobasic
3. Sodium Bicarbonate
4. Sodium Chloride
5. Sodium Phosphate, dibasic

Additionally, MEM in Hanks’ Buffer contains:

• Low level of glucose (1.00 g/ liter)
• Sodium bicarbonate (0.35 g/L). If , however, higher than 5% CO2 is used, a higher % of sodium bicarbonate is needed.
• 2 mM L-glutamine (292 mg/L)

Moreover, PurMa Biologics manufactures 122 types of MEM.

Why PurMa Biologics? Why we stand behind our cell culture media

Our products are the outcome of 30 years of experience in cutting edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. Moreover, We utilize %99 pure amino acids. This will significantly increase the consistency among your experiments. Considering the highest quality, our prices certainly are extremely reasonable.

Applications

MEM has been used for the cultivation of a wide variety of cells grown in monolayers attached or in suspension cell lines such as HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts as well as primary rat astrocytes. MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% fetal bovine serum (FBS).

Formulation

Complete formulation click here as well as in Formulation Tab: MEM (HBSS) Formulation in Hanks' Buffer

References

1. Culture of granulosa cells in collagen gels: the influence of cell shape on steroidogenesis. Carnegie et al. Biol Reprod. 1988 May;38(4):881-90. doi: 10.1095/biolreprod38.4.881. PMID: 3401543
2. Myelin formation in rat dorsal root ganglion cultured in a serum-free medium. Ninomiya et al. J Anat. 1987 Jun;152:209-13. PMID: 3654371

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