Minimum Essential Media (MEM) In Hanks’ Buffer
Background
Minimum Essential Media (MEM) In Hanks’ Buffer is a modification of Basal Medium Eagle . It was originally developed by Harry Eagle for specific requirements of certain subtypes. Additionally, it includes higher concentrations of amino acids which is more suitable for cultured mammalian cells. Moreover, PurMa Biologics manufactures MEM with Earle’s buffer for use in a CO2 incubator, or with Hanks’ salts for use without CO2.
PurMa™ MEM media in Hank’s buffer includes Hank’s buffer
Hanks buffer, it contains:
- Potassium Chloride
- Potassium Phosphate, monobasic
- Sodium Bicarbonate
- Sodium Chloride
- Sodium Phosphate, dibasic
Additionally, MEM in Hanks’ Buffer contains:
- Low level of glucose (1.00 g/ liter)
- Sodium bicarbonate (0.35 g/L). If , however, higher than 5% CO2 is used, a higher % of sodium bicarbonate is needed.
- 2 mM L-glutamine (292 mg/L)
Moreover, PurMa Biologics manufactures 122 types of MEM.
Applications
MEM has been used for the cultivation of a wide variety of cells grown in monolayers attached or in suspension cell lines such as HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts as well as primary rat astrocytes. MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% fetal bovine serum (FBS).
Formulation
Complete formulation click here as well as in Formulation Tab: MEM (HBSS) Formulation in Hanks’ Buffer
References
- Culture of granulosa cells in collagen gels: the influence of cell shape on steroidogenesis. Carnegie et al. Biol Reprod. 1988 May;38(4):881-90. doi: 10.1095/biolreprod38.4.881. PMID: 3401543
- Myelin formation in rat dorsal root ganglion cultured in a serum-free medium. Ninomiya et al. J Anat. 1987 Jun;152:209-13. PMID: 3654371
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- Minimum Essential Media (MEM) In Hanks’ Buffer, Cell Culture Media
Advanced Minimum Essential Media (A-MEM) in HBSS
- Advanced Minimum Essential Media (A-MEM) in Hanks’ Buffer contains L-Alanyl-L-Glutamine as well as ingredients to allow for serum reduction. Additionally, Advanced MEM needs 2-5% FBS and provides more consistency.
- Read more
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- Minimum Essential Media (MEM) In Hanks’ Buffer, Cell Culture Media
Minimum Essential Media (MEM) In Hanks’ Buffer
- Minimum Essential Media (MEM) In Hanks’ Buffer is a modification of Basal Medium Eagle. This media includes higher concentrations of amino acids making it suitable for cultured mammalian cells. Moreover, PurMa Biologics manufactures MEM with Earle’s buffer for use in a CO2 incubator, or with Hanks’ salts for use without CO2.
- Read more
-
- Minimum Essential Media (MEM) In Hanks’ Buffer, Cell Culture Media
Minimum Essential Media-MAX (MEM-MAX) in Hanks’ Buffer
- Minimum Essential Media MAX (MEM-MAX) in Hanks’ Buffer contains L-Alanyl-L-Glutamine, therefore supplying a longer shelf life. MEM-MAX in HBBS significantly improves cell viability and growth. We manufacture several types of MEM-MAX based on your requirements.
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