Basal Medium Eagle (BME) in Earle’s Buffer
Background
Basal Medium Eagle (BME) in Earle’s Buffer was originally developed by Harry Eagle. this media is one of the most widely used of all synthetic cell culture media. Furthermore, BME, when properly supplemented, has demonstrated wide applicability, for supporting monolayer growth of a wide variety of normal and transformed cell lines. Moreover, PurMa Biologics manufactures BME with Earle’s as well as in Hanks’ salts for use without CO2.
Ingredients
PurMa™ BME media in Earle’s buffer contains:
Earl’s buffer includes:
- Calcium chloride
- Magnesium sulfate
- Potassium chloride
- Sodium bicarbonate
- Sodium chloride
- and lastly, Sodium Phosphate, monobasic
PurMa Biologics so far, manufactures 109 types BME in Earle’s buffer.
Why PurMa Biologics? Why we stand behind our cell culture media.
Our products are the outcome of 30 years of experience in cutting-edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. Moreover, We utilize %99 pure amino acids. This will significantly increase the consistency of your experiments. Undoubtedly, considering the highest quality, our prices certainly are extremely reasonable.
Application
BME provides an excellent nutritional capacity for suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts as well as primary rat astrocytes.
Formulation
for complete formulation click here, alternatively, you can find it in the “Formulation Tab”: BME with Earle’s Buffer Formulation
References:
- Improved chemically defined basal medium (CMRL-1969) for primary monkey kidney and human diploid cells. Healy GM, Teleki S, von Seefried A, Walton MJ, Macmorine HG. Appl Microbiol. 1971 Jan;21(1):1-5. doi: 10.1128/am.21.1.1-5.1971.
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- Basal Medium Eagle (BME) in Earle’s Buffer, Cell Culture Media
Advanced Basal Medium Eagle (A-BME) in Earle’s Buffer
- Advanced Basal Medium Eagle (A-BME) in Earle’s Buffer contains L-Alanyl-L-Glutamine as well as ingredients to allow for serum reduction. Additionally, Advanced BME needs 2-5% FBS and provides more consistency.
- Read more
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- Basal Medium Eagle (BME) in Earle’s Buffer, Cell Culture Media
Basal Medium Eagle (BME) in Earle’s Buffer
- Basal Medium Eagle (BME) in Earl’s Buffer developed by Harry Eagle, and certainly is one of the most widely used of all synthetic cell culture media. BME has demonstrated widely applicable for supporting monolayer cell growth in wide variety of normal as well as transformed cell lines.
- Read more
-
- Basal Medium Eagle (BME) in Earle’s Buffer, Cell Culture Media
Basal Medium Eagle-MAX (BME-MAX) in Earle’s Buffer
- Basal Medium Eagle-MAX (BME-MAX) in Earle’s Buffer contains L-Alanyl-L-Glutamine, therefore supplying a longer shelf life. BME-MAX in EBBS significantly improves cell viability and growth. We manufacture several types of BME-MAX based on your requirements.
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- Basal Medium Eagle (BME) in Earle’s Buffer
Leibovitz’s L-15 Medium
- Leibovitz's L-15 Medium was originally designed to be used in carbon dioxide (CO2) free systems. Therefore, does not need sodium bicarbonate to provide the buffering capacity. Instead, phosphates, free base amino acids, salts, and galactose maintain the physiological pH.
- Read more
