Leibovitz’s L-15 Medium

Leibovitz’s L-15 Medium was originally designed to be used in carbon dioxide (CO2) free systems. Therefore, does not need sodium bicarbonate to provide the buffering capacity. Instead, phosphates, free base amino acids, salts, and galactose maintain the physiological pH.

Description

Leibovitz’s L-15 Medium

Background

Leibovitz’s L-15 Medium was originally designed to be used in carbon dioxide (CO2) free systems. Therefore, does not need sodium bicarbonate to provide the buffering capacity. Instead, phosphates, free base amino acids, salts, and galactose maintain the physiological pH.

This media contains:

  • High Level of Sodium pyruvate (5mM)
  • Undoubtedly, the absence of Sodium Bicarbonate makes this media unique.
  • Basically, the buffering Capacity is maintained by other elements, mainly phosphates.
  • And finally Using Galactose instead of glucose makes this media quite different.

Application of Leibovitz’s L-15

  • Cryopreservation of cells and tissues such as Xenopus sperm and embryonic cells.
  • Establishing human tumor cells lines such as Hep-2 cells.
  • As a supplement in sterile cell media for the dissection and isolation of kidney cells.
  • As an important media which provides proper buffering system for culturing of lung cells.
  • A valid option for growing fish originated from cell lines.

PurMa Biologics manufactures 107 types of Leibovitz’s L-15

Why PurMa Biologics? Why we stand behind our cell culture media

Our products are the outcome of 30 years of experience in cutting edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. We utilize %99 pure amino acids. This will significantly increase the consistency among your experiments, and the result of your lab. Considering the highest quality, our prices are extremely reasonable.

Formulation

For complete formulation please visit the “formulation tab”, as well as by clicking here: Leibovitz’s L-15 Formulation

References

  1. The effect of a phorbol ester on amphibian myogenesis. Zeng MB, Tseng MP. Shi Yan Sheng Wu Xue Bao. 1990 Mar;23(1):106-15. PMID: 2382522.
  2. Expression of differentiation molecules is preserved in human fetal kidneys during culture. Brière et al. Acta Histochem. 1990;89(2):157-66. doi: 10.1016/s0065-1281(11)80351-x.PMID: 2093265.
Parameter Specification
Appearance Red, clear liquid
pH 7.2 ± 0.1
Osmolality 275-360 mOsm/L
Endotoxin NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive Sodium pyruvate
Indicator Phenol red
Mycoplasma Detection Negative
Sterility Tested Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

 

Additional information

Condition

Leibovitz's L-15 Standard Formulation, Leibovitz's L-15 w/o Glutamine, Leibovitz's L-15 w/o Phenol Red, Leibovitz's L-15 w/o Sodium Pyruvate, Leibovitz's L-15 w/o Galactose, Leibovitz's L-15 w/o Glutamine, w/o Phenol Red, Leibovitz's L-15 w/o Glutamine, w/o Sodium Pyruvate, Leibovitz's L-15 High Galactose (4.5 g/L), Leibovitz's L-15 15 mM HEPES (3.6 g/L), Leibovitz's L-15 25 mM HEPES (5.9 g/L)

Format

Liquid, Powder

Size

10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed

Quality Control

[vc-quality-control-tab]

Formulation

Leibovitz’s L-15

Reviews

There are no reviews yet.

Only logged in customers who have purchased this product may leave a review.