Leibovitz’s L-15 Medium was originally designed to be used in carbon dioxide (CO2) free systems and therefore does not need sodium bicarbonate to provide the buffering capacity. Instead, phosphates, free base amino acids, salts, and galactose maintain the physiological pH.

Application of Leibovitz’s L-15:

  • Cryopreservation of cells and tissues such as Xenopus sperm and embryonic cells.
  • Establishing human tumor cells lines such as Hep-2 cells.
  • As a supplement in sterile cell media for the dissection and isolation of kidney cells.
  • As an important media which provides proper buffering system for culturing of lung cells.
  • A valid option for growing fish originated cell lines

This media has unique criteria:

  • High Level of Sodium pyruvate (5mM)
  • Absence of Sodium Bicarbonate which is commonly used in other media for maintaining buffering capacity.
  • Using Galactose instead of glucose

Leibovitz’s L-15 contains no proteins or growth factors and therefore should be supplemented with FBS.

PurMa Biologics manufactures 107 types of Ham’s F-12K which accommodate any form of this medium. We recommend contacting our friendly customer service & technical support about your exact need for Ham’s F-12K   media.

Why PurMa Biologics? Why we stand behind our cell culture media:

  1. Our products are the outcome of 30 years of experience in cutting edge cell and tissue culture science.
  2. We routinely manufacture over 1500 media which is the most comprehensive collection of cell and tissue culture media.
  3. We utilize %99 pure amino acids. This will significantly increase the consistency among your experiments, and the result of your lab compared to other laboratories working on the same issues.
  4. Considering the highest quality, our prices are extremely reasonable.

Ham’s F-12K   Medium contains no proteins, lipids, or growth factors. Therefore, Ham’s F-12 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS).

Formulation: for complete formulation click here: Leibovitz’s L-15

Parameter Specification
Appearance Red, clear liquid
pH 7.2 ± 0.1
Osmolality 275-360 mOsm/L
Endotoxin NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive Sodium pyruvate
Indicator Phenol red
Mycoplasma Detection Negative
Sterility Tested Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature