Basal Medium Eagle-MAX (BME-MAX) in Earle’s Buffer

Basal Medium Eagle-MAX (BME-MAX) in Earle’s Buffer contains L-Alanyl-L-Glutamine, therefore supplying a longer shelf life.   BME-MAX in EBBS significantly improves cell viability and growth. We manufacture several types of BME-MAX based on your requirements.

Description

Basal Medium Eagle-MAX (BME-MAX) in Earle’s Buffer

Background

Basal Medium Eagle-MAX (BME-MAX) in Earle’s Buffer is a commonly used media for Mammalian cell culture.

Advantages

The advantages of BME-MAX over BME are:

  • Higher PH Capacity
  • Moreover, it contains 4 mM L-Alanyl-L-Glutamine (0.869 g/L) instead of L-Glutamine and therefore:
  • Possess longer shelf life.
  • More importantly, minimizes toxic ammonia build-up.
  • Significantly improves cell viability and growth.
  • Remains stable across a wide range of temperatures.
  • Improves the culture of primary cells.

Mechanism

Although L-glutamine is a vital amino acid, it degrades, generating toxic ammonia and pyrrolidine carboxylic acid. One way to minimize L-glutamine degradation in media is to gradually add these amino acids to your cell culture. However, keeping track of time to keep the amount of L-glutamine at the same level is tedious, if not impossible.

Alternatively, L-alanyl-L-glutamine can be used because it is much more stable in aqueous solutions. More importantly, it does not easily degrade and gradually releases aminopeptidases. As a result, L-glutamine and L-alanine are then used by the cells for protein production in the TCA cycle.

In addition to our Max formulation products, we manufacture PurMaTM GluaSup (Cat# P3S210 559) is a combination of L-alanyl-L-glutamine that prevents degradation of L-glutamine.

Contents

Basal Medium Eagle-MAX (BME-MAX) in Earle’s Buffer contains:

  • 5 mM sodium pyruvate
  • High glucose (4.5g/L)
  • Non-Essential Amino Acids
  • 3.7 g/L Sodium Bicarbonate; PurMa Biologics Manufactures several types of BME-MAX in EBBS based on the amount of Sodium Bicarbonate and HEPES (PH capacity)
  • Phenol Red

BME-MAX in EBBS contains no proteins, lipids, or growth factors. Therefore, it requires supplementation, commonly with 10% Fetal bovine Serum (FBS).

Formulation

The complete formulation can be found here: BME-MAX with Earl’s Buffer Formulation

References

  1. Improved chemically defined basal medium (CMRL-1969) for primary monkey kidney and human diploid cells. Healy GM, et al.  Appl Microbiol. 1971 Jan;21(1):1-5. doi: 10.1128/am.21.1.1-5.1971. PMID:
  2. Nutritional requirements for the production of herpes simplex virus. I. Influence of glucose and glutamine of herpes simplex virus production by HeLa cells. LEWIS VJ Jr, SCOTT LV. J Bacteriol. 1962 Mar;83(3):475-82. doi: 10.1128/jb.83.3.475-482.1962. PMID: 14464909
  3. An improved culture system for secondary palatal elevation. Lewis CA, et al. In Vitro. 1980 Jun;16(6):453-60. doi: 10.1007/BF02626457. PMID: 7390537

 

Parameter Specification
Appearance Red, clear liquid
pH 7.2 ± 0.1
Osmolality 275-360 mOsm/L
Endotoxin NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive N/A
Indicator Phenol red
Mycoplasma Detection Negative
Sterility Tested Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

 

Additional information

Condition

BME-MAX Standard Formulation, BME-MAX w/o Phenol Red, BME-MAX w/o Sodium Bicarbonate, BME-MAX w/o Glucose, BME-MAX w/o Phenol Red, w/o Sodium Bicarbonate, BME-MAX 15 mM HEPES (3.6 g/L), BME-MAX 25 mM HEPES (5.9 g/L), BME-MAX Sodium Pyruvate (0.11 g/L), BME-MAX Low Sodium Bicarbonate (1.2 g/L), BME-MAX High Glucose (4.5 g/L), BME-MAX 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.1 g/L), BME-MAX 25 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.1 g/L)

Format

Liquid, Powder

Size

10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed

Quality Control

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Formulation

BME-MAX with Earl’s Buffer Formulation

 

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