Advanced Basal Medium Eagle (A-BME) in Earle’s Buffer

Advanced Basal Medium Eagle (A-BME) in Earle’s Buffer contains L-Alanyl-L-Glutamine as well as ingredients to allow for serum reduction.  Additionally, Advanced BME needs 2-5% FBS and provides more consistency.

Description

Advanced Basal Medium Eagle (A-BME) in Earle’s Buffer

Background

PurMaTM Advanced Basal Medium Eagle (A-BME) in Earle’s Buffer contains components that allow for a 60–90% reduction in FBS supplementation. More importantly, using synthetic components such as recombinant insulin, albumin, and transferrin increase consistency. This results in less variation amongst lot-to-lot serum changes. In A-BME, L-Alanyl-L-Glutamine is used as the source of glutamine. Additionally, reducing FBS requirements lowers the cost of cell culture experiments.

Contents

Advanced BME in EBSS Contains:

  • includes all twenty essential and non-essential amino acids with higher concentrations than regular and MAX formulations.
  • A-BME incorporates elements that significantly reduce the need for a serum for cell culture.

Crucial Ingredients:

  • PurMaTM Human Transferrin (Holo): prevents Improper iron storage and delivery in cell culture systems which is a major contributor to oxidative stress and protein damage.
  • PurMaTM Insulin Recombinant
  • Trace of other elements which mimic the elements in fetal bovine serum.

Consequently, Advanced Basal Medium Eagle (A-BME) in Earle’s Buffer is a significant step toward a chemically defined form of this media. Unquestionably, Chemically Defined Advanced BME (CD-A-BME) is the outcome of decades of cutting-edge research and will soon be offered globally to laboratories that are looking for consistency and repeatability in their data.

Formulation

Complete formulation is available here: Advanced BME in Earle’s Buffer

References

  1. Improved chemically defined basal medium (CMRL-1969) for primary monkey kidney and human diploid cells. Healy GM, et al. Appl Microbiol. 1971 Jan;21(1):1-5. doi: 10.1128/am.21.1.1-5.1971.
  2. Nutritional requirements for the production of herpes simplex virus. I. Influence of glucose and glutamine of herpes simplex virus production by HeLa cells. LEWIS VJ Jr, SCOTT LV. J Bacteriol. 1962 Mar;83(3):475-82. doi: 10.1128/jb.83.3.475-482.1962. PMID: 14464909
  3. An improved culture system for secondary palatal elevation. Lewis CA, Thibault L, Pratt RM, Brinkley LL. In Vitro. 1980 Jun;16(6):453-60. doi: 10.1007/BF02626457. PMID: 7390537

 

Parameter Specification
Appearance Red, clear liquid
pH 7.2 ± 0.1
Osmolality 275-360 mOsm/L
Endotoxin NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive Sodium pyruvate
Indicator Phenol red
Mycoplasma Detection Negative
Sterility Tested Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

 

Additional information

Condition

A-BME Standard Formulation, A-BME w/o L-Alanyl-L-Glutamine, A-BME w/o Phenol Red, A-BME w/o Sodium Bicarbonate, A-BME w/o Glucose, A-BME w/o L-Alanyl-L-Glutamine, w/o Phenol Red, A-BME w/o L-Alanyl-L-Glutamine, w/o Sodium Bicarbonate, A-BME w/o Phenol Red, w/o Sodium Bicarbonate, A-BME w/o L-Alanyl-L-Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, A-BME 15 mM HEPES (3.6 g/L), A-BME 25 mM HEPES (5.9 g/L), A-BME Low Sodium Bicarbonate (1.2 g/L), A-BME High Glucose (4.5 g/L), A-BME 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.1 g/L), A-BME 25 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.1 g/L)

Format

Liquid, Powder

Size

10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed

Quality Control

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Formulation

Advanced BME in Earl’s Buffer Formulation

SDS

SDS

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