Advanced Basal Medium Eagle (A-BME) in Earle’s Buffer
Advanced Basal Medium Eagle (A-BME) in Earle’s Buffer contains L-Alanyl-L-Glutamine as well as ingredients to allow for serum reduction. Additionally, Advanced BME needs 2-5% FBS and provides more consistency.
Description
Advanced Basal Medium Eagle (A-BME) in Earle’s Buffer
Background
PurMaTM Advanced Basal Medium Eagle (A-BME) in Earle’s Buffer contains components that allow for a 60–90% reduction in FBS supplementation. More importantly, using synthetic components such as recombinant insulin, albumin, and transferrin increase consistency. This results in less variation amongst lot-to-lot serum changes. In A-BME, L-Alanyl-L-Glutamine is used as the source of glutamine. Additionally, reducing FBS requirements lowers the cost of cell culture experiments.
Contents
Advanced BME in EBSS Contains:
- includes all twenty essential and non-essential amino acids with higher concentrations than regular and MAX formulations.
- A-BME incorporates elements that significantly reduce the need for a serum for cell culture.
Crucial Ingredients:
- PurMaTM Human Transferrin (Holo): prevents Improper iron storage and delivery in cell culture systems which is a major contributor to oxidative stress and protein damage.
- PurMaTM Insulin Recombinant
- Trace of other elements which mimic the elements in fetal bovine serum.
Consequently, Advanced Basal Medium Eagle (A-BME) in Earle’s Buffer is a significant step toward a chemically defined form of this media. Unquestionably, Chemically Defined Advanced BME (CD-A-BME) is the outcome of decades of cutting-edge research and will soon be offered globally to laboratories that are looking for consistency and repeatability in their data.
Formulation
Complete formulation is available here: Advanced BME in Earle’s Buffer
References
- Improved chemically defined basal medium (CMRL-1969) for primary monkey kidney and human diploid cells. Healy GM, et al. Appl Microbiol. 1971 Jan;21(1):1-5. doi: 10.1128/am.21.1.1-5.1971.
- Nutritional requirements for the production of herpes simplex virus. I. Influence of glucose and glutamine of herpes simplex virus production by HeLa cells. LEWIS VJ Jr, SCOTT LV. J Bacteriol. 1962 Mar;83(3):475-82. doi: 10.1128/jb.83.3.475-482.1962. PMID: 14464909
- An improved culture system for secondary palatal elevation. Lewis CA, Thibault L, Pratt RM, Brinkley LL. In Vitro. 1980 Jun;16(6):453-60. doi: 10.1007/BF02626457. PMID: 7390537
| Parameter | Specification |
|---|---|
| Appearance | Red, clear liquid |
| pH | 7.2 ± 0.1 |
| Osmolality | 275-360 mOsm/L |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Suitability | Suitable for mammalian cell culture |
| Additive | Sodium pyruvate |
| Indicator | Phenol red |
| Mycoplasma Detection | Negative |
| Sterility Tested | Sterile filtered using 0.22 µm filter |
| Form | Liquid |
| Shipping Condition | Room temperature |
Additional information
| Condition | A-BME Standard Formulation, A-BME w/o L-Alanyl-L-Glutamine, A-BME w/o Phenol Red, A-BME w/o Sodium Bicarbonate, A-BME w/o Glucose, A-BME w/o L-Alanyl-L-Glutamine, w/o Phenol Red, A-BME w/o L-Alanyl-L-Glutamine, w/o Sodium Bicarbonate, A-BME w/o Phenol Red, w/o Sodium Bicarbonate, A-BME w/o L-Alanyl-L-Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, A-BME 15 mM HEPES (3.6 g/L), A-BME 25 mM HEPES (5.9 g/L), A-BME Low Sodium Bicarbonate (1.2 g/L), A-BME High Glucose (4.5 g/L), A-BME 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.1 g/L), A-BME 25 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.1 g/L) |
|---|---|
| Format | Liquid, Powder |
| Size | 1 x 10 L (Powder), 1 X 50 L (Powder), 10 X 1EZ Individually Packed (Powder), 50 X 1EZ Individually Packed (Powder), 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml |
Quality Control
[vc-quality-control-tab]Formulation
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