Williams′ Medium E is a modification of Williams′ Medium D by Williams and Gunn. The composition is similar to Minimum Essential Medium (MEM) with some difference in glucose as well as amino acid contents.  Williams′ Medium E was originally used to isolate and culture primary liver epithelial cells from Epithelial-like cells of fibroblast-like cells culture. It has been originally shown by Williams and Gunn that the cells maintained their morphology for up to nine months of continuous culture and displayed no tumorigenicity upon injection into syngeneic hosts. So, Williams′ Medium E medium is suited for longer term adult primary cell culture.

PurMa™ William’s E Medium is:

  • Suited for long term culture.
  • Enriched in amino acids.
  • Double the glucose compared to the original formulation.
  • Contains unique ingredients, including zinc, iron, manganese, non-essential amino acids, the reducing agent glutathione, and the lipid methyl linoleate which, in part, contribute to the ability of this medium to support long term culture.

PurMa™ William’s E Medium also contains:

–  Low level of glucose (2.00 g/ liter). PurMa Biologics manufacturers the high glucose (4.5 g/L) Williams′ Medium E as well (Cat #: P3S029880)

–  Sodium bicarbonate (2.2 g/L) (if higher than 5% CO2 is used, a higher % of sodium bicarbonate is needed). PurMa Biologics manufacturers the high sodium bicarbonate (3.7 g/L) Williams′ Medium E as well (Cat #: P3S032880)

–  2 mM L-glutamine (292 mg/L)’

PurMa Biologics manufactures 112 types of William’s E Medium which accommodate any form of this medium. We recommend contacting our friendly customer service & technical support about your exact need for William’s E Medium media.

Why PurMa Biologics? Why we stand behind our cell culture media:

  1. Our products are the outcome of 30 years of experience in cutting edge cell and tissue culture science.
  2. We routinely manufacture over 1500 media which is the most comprehensive collection of cell and tissue culture media.
  3. We utilize %99 pure amino acids. This will significantly increase the consistency among your experiments, and the result of your lab compared to other laboratories working on the same issues.
  4. Considering the highest quality, our prices are extremely reasonable.

Application of William’s E Medium

This media has elements supporting long term culturing of primary cells as a necessity for performing analysis on these cells. William’s E Medium has been formulated for use in a 5% carbon dioxide atmosphere and is not recommended for higher and lower percentages of CO2. It utilizes a bicarbonate buffering system. Scientists in PurMa Biologics have successfully evaluated William’s E Medium for the culture of vast varieties of primary as well as commercial cell lines including: primary liver epithelial cells, HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, carcinomas, and hybridoma cells. William’s E Medium contains no proteins, lipids, or growth factors. Therefore, William’s E Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS).

Formulation: for complete formulation click here:Regular William E Formulation

Parameter Specification
Appearance Red, clear liquid
pH 7.2 ± 0.1
Osmolality 275-360 mOsm/L
Endotoxin NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive Sodium pyruvate
Indicator Phenol red
Mycoplasma Detection Negative
Sterility Tested Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature