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kaighn's f-12 medium

PurMa™ Kaighn’s Modification of Ham’s F-12-K Medium

PurMa™ Kaighn’s Modification of Ham’s F-12-K Medium is a specialized cell culture medium designed to enhance the growth and maintenance of various cell types, providing essential nutrients and optimized conditions for cellular proliferation and experimentation.

  • ✓Proudly made in the USA, manufactured in house
  • ✓Certificate of Analysis available for every lot
  • ✓Backed by decades of cell culture and protein biochemistry expertise
  • ✓Bulk pricing, purchase orders, and custom formulations welcome
Questions or need a quote? Contact our team or call (866) 720-6116.
SKU: Kaighn-GUA17 Categories: Cell Culture Media, Kaighn's Modification of Ham's F-12K Medium Tags: Ham's F-12, Ham's F-12K, Kaighn's

Description

PurMa™ Kaighn’s Modification of Ham’s F-12-K Medium

Product Description

PurMa™ Kaighn’s Modification of Ham’s F‑12 (F‑12K) is a historically proven, enriched basal medium. This medium was originally formulated for primary cells (e.g., hepatocytes, oocytes) and is now extensively used across many mammalian cell types. Kaighn’s Modification of Ham’s F-12-K Medium is formulated with components absent in older media — including putrescine, thymidine, hypoxanthine, and zinc — and contains higher levels of amino acids, vitamins, inositol, and choline to support growth in low‑serum or serum‑free conditions. It has been shown that Ham F12K is promising in rat and chicken  primary cell culture. F-12K is a modification of Ham’s F‑12 medium.

Key formulation highlights

  • Low glucose: ~1.26 g/L (suitable for glucose‑sensitive cells).
  • Sodium bicarbonate: standard 2.5 g/L (alternative formulations available: 1.5 g/L Cat# P3S057108 and 3.7 g/L Cat# P3S032108 to match different CO2 settings).
  • L‑glutamine: 2 mM (≈292 mg/L).
  • Unique additives: putrescine, thymidine, hypoxanthine, zinc — support nucleotide metabolism and reduce clumping.
  • Elevated concentrations: amino acids, vitamins, inositol and choline for robust proliferation and viability.
  • Manufactured using >99% pure amino acids and stringent QC for lot‑to‑lot consistency.

Applications

  • Primary cells: hepatocytes, oocytes, and other sensitive primary cultures.
  • Cell lines: HeLa, Jurkat, MCF‑7, PC12, PBMCs, astrocytes, hybridomas, bone marrow cultures, and other adherent/suspension cells.
  • Use cases: routine maintenance, proliferation assays, transfection, low‑serum culture, and sensitive functional assays.

Why choose PurMa™ Kaighn’s Modification of Ham’s F-12-K Medium

  • Decades of formulation expertise and a broad media library (1,500+ media) for application support.
  • High‑purity components increase reproducibility across labs and experiments.
  • Multiple NaHCO3 options to match CO2 incubator conditions and maintain pH stability.
  • Competitive pricing with comprehensive documentation (COA available).

Storage & handling

  • Store unopened at 2–8 °C; protect from light.
  • Supplement with serum or growth factors per your protocol.
  • Use aseptic technique; follow your lab SOPs and run routine mycoplasma and contamination checks.

Call to action Request a Certificate of Analysis (COA), sample, or technical support for protocol optimization.

FAQs

Q1: What is Kaighn’s Modification of Ham’s F-12-K Medium) medium used for?
A1: Kaighn’s Modification of Ham’s F-12-K Medium is used for culturing primary cells (e.g., hepatocytes, oocytes) and many established cell lines. Its enriched composition reduces clumping and supports growth in low‑serum conditions.

Q2: What are the sodium bicarbonate options for PurMa F‑12K?
A2: PurMa offers Kaighn’s Modification of Ham’s F-12-K Medium with standard 2.5 g/L NaHCO3 and alternative formulations with 1.5 g/L (Cat# P3S057108) and 3.7 g/L (Cat# P3S032108) to match different CO2 incubator settings.

Q3: Does F‑12K contain glutamine?
A3: Yes. Standard PurMa F‑12K contains 2 mM L‑glutamine (≈292 mg/L). Handle and store according to lab SOPs.

Q4: Which cell types grow well in kaighn’s f-12 medium?
A4: Examples include hepatocytes, oocytes, HeLa, Jurkat, MCF‑7, PC12, PBMCs, astrocytes, hybridomas, and other primary and transformed cells.

Q5: How should I store and handle PurMa kaighn’s f-12 medium?

A5: Store unopened at 2–8 °C, protect from light, and supplement per your protocol. Avoid repeated freeze‑thaw cycles and use aseptic technique.

Formulation

For complete formulation click here: Ham’s F-12K Formulation

References

  1. Growth and continuous passage of COMMA-D mouse mammary epithelial cells in hormonally defined serum-free medium. Riss et al. Cancer Res. 1987 Jul 15;47(14):3776-82. PMID: 3297308.
  2. Human recombinant insulin-like growth factor I. I. Development of a serum-free medium for clonal density assay of growth factors using BALB/c 3T3 mouse embryo fibroblasts. Riss et al. In Vitro Cell Dev Biol. 1988 Nov;24(11):1099-106. doi: 10.1007/BF02620811.
  3. Hormones and factors that stimulate growth of a rat islet tumor cell line in serum-free medium. Fong et al. Diabetes. 1981 Dec;30(12):1022-8. doi: 10.2337/diab.30.12.1022. PMID: 6273246
Parameter Specification
Appearance Red, clear liquid
pH  7.2 ± 0.1
Osmolality  275-360 mOsm/L
Endotoxin  NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive  Sodium pyruvate
Indicator  Phenol red
Mycoplasma Detection Negative
Sterility Tested  Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

Learn more: What Is Ham’s F-12K?

Additional information

Condition

HAM’S F-12K Standard Formulation, HAM’S F-12K Standard Formulation, ATCC Formulation, HAM’S F-12K w/o Glutamine, HAM’S F-12K w/o Phenol Red, HAM’S F-12K w/o Sodium Bicarbonate, HAM’S F-12K w/o Sodium Pyruvate, HAM’S F-12K w/o Glucose, HAM’S F-12K w/o Lipoic Acid, HAM’S F-12K w/o Glutamine, w/o Phenol Red, HAM’S F-12K w/o Phenol Red, w/o Sodium Bicarbonate, HAM’S F-12K w/o Sodium Bicarbonate, w/o Sodium Pyruvate, HAM’S F-12K w/o Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, HAM’S F-12K High Glucose (4.5 g/L), HAM’S F-12K Low Sodium Bicarbonate (1.5 g/L), HAM’S F-12K High Sodium Bicarbonate (3.7 g/L), HAM’S F-12K 15 mM HEPES (3.6 g/L), HAM’S F-12K 25 mM HEPES (5.9 g/L), HAM’S F-12K 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.5 g/L), HAM’S F-12K 25 mM HEPES (5.9 g/L), Low Sodium Bicarbonate (1.5 g/L), HAM’S F-12K w/o Sodium Pyruvate, w/o Phenol Red, HAM’S F-12K w/o Phenol Red, with 15 mM HEPES (3.6 g/L), HAM’S F-12K w/o Phenol Red, with 25 mM HEPES (5.9 g/L), HAM’S F-12K w/o Phenol Red, Low Sodium Bicarbonate (1.5 g/L), HAM’S F-12K w/o Sodium Pyruvate, Low Sodium Bicarbonate (1.5 g/L)
Format

Liquid, Powder
Size

10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L

Documents

Certificate of Analysis (COA): Available upon request for your specific order — email info@purmabiologics.com and we will send the current lot documentation.

Safety Data Sheet (SDS): Available upon request — email info@purmabiologics.com.

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