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- ✓Certificate of Analysis available for every lot
- ✓Backed by decades of cell culture and protein biochemistry expertise
- ✓Bulk pricing, purchase orders, and custom formulations welcome
Eukaryotic Cell Culture Suited Antibiotics
PurMa Biologics Eukaryotic Cell Culture Suited Antibiotics. Our antibiotics mix offers a broad range of protection for mammalian and in general Eukaryotic cell lines. Our Antibiotic Mixes are prepared in PurMa™ Ultra-Pure Cell Culture Suited Water.
Description
Background
PurMa Biologics offers Eukaryotic Cell Culture Suited Antibiotics mix. Moreover, we manufacture a wide collection of antibiotic types. Additionally, our Cell Culture Suited Antibiotics offer protection for specific cell lines (see below). Notably, the main function of each is summarized in the table below and the full description and complete mechanism of action can be found in the mentioned literature, Click here: PurMa Antibiotics Booklet for Eucaryotes
Specific Criteria
- It does not contain any PH-manipulating ingredients
- After preparation, we sterilize the antibiotic mix by passing it through 0.1 µm filters.
- We pack our antibiotic mix under N2
Furthermore, we manufacture PurMa™ Cell Culture Suited Antibiotic Mixes in PurMa™ Ultra-Pure Cell Culture Suited Water. For more details click here: PurMa™ Ultra-Pure Cell Culture Suited water
Guideline
Formulations and preparation guidelines, click here: PurMa Antibiotics Guidelines for Mammalian Cells_Eukaryotes
References
- Efficient selection for high-expression transfectants with a novel eukaryotic vector. Niwa H, Yamamura K, Miyazaki J. Gene. 1991 Dec 15;108(2):193-9. doi: 10.1016/0378-1119(91)90434-d. PMID: 1660837.
- Neomycin Sulfate Improves the Antimicrobial Activity of Mupirocin-Based Antibacterial Ointments. Blanchard C, Brooks L, Beckley A, Colquhoun J, Dewhurst S, Dunman PM. Antimicrob Agents Chemother. 2015 Nov 23;60(2):862-72. doi: 10.1128/AAC.02083-15. Print 2016 Feb. PMID: 26596945.
Additional information
| Antibiotic | Amphotericin B (100X), 250 µg/ml, Dihydrostreptomycin sesquisulfate (100X), 10 mg/ml, Erythromycin (100X), 10 mg/ml, G 418, 10 mg/ml, Gentamicin Sulfate (1000X), 50 mg/ml, Neomycin Sulfate (1000X), 50 mg/ml, Nystatin (100X), 5 mg/ml, Paromomycin Sulfate (1000X), 100 mg/ml, Penicillin-G (potassium salt) (100X) (Benzylpenicillin), 10000 U, Penicillin-G (sodium salt) (100X), 10000 U, Phenoxymethylpenicillinic Acid (potassium salt) [Penicillin V] (100X), 10000 U, Polymyxin B Sulfate (100X), 0.5 mg/ml, Spectinomycin dihydrochloride pentahydrate (100X), 2 mg/ml, Streptomycin sulfate (100X), 10 mg/ml, Tetracycline Hydrochloride (100X), 1 mg/ml, Tylosin Tartrate (8 mg/ml soln.) (1000X), 1 mg/ml |
|---|---|
| Size | 20 ml, 50 ml, 100 ml |
Documents
Certificate of Analysis (COA): Available upon request for your specific order — email info@purmabiologics.com and we will send the current lot documentation.
Safety Data Sheet (SDS): Available upon request — email info@purmabiologics.com.
What our customers say about PurMa Biologics
Feedback from labs we supply, across our full catalogue.
We have been working with PurMa biologics for the past several years, we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum. We appreciate the high quality and reasonable price, and great customer service.
We purchase everything we need related to cell and tissue culture from PurMa, reagents like: DMEM, LL15, EMEM, FBS, trypsin, antibiotics mix100X and... Always great quality, fast processing and shipping. We are totally satisfied.
Very fast, efficient, and economic service. Great for new PIs like me.
it is reliable vendor!
We always buy our agarose from PurMa and have never been disappointed.
I recently had the pleasure of interacting with Dr. Seif Azadi from Purma Biologics, and I cannot thank him enough for his expertise and prompt assistance. His deep knowledge and dedication were instrumental in meeting our laboratory’s needs with remarkable efficiency. We ordered FBS, DMEM, and Trypsin from Purma Biologics, and I’m thrilled to report that all the products have performed exceptionally well. They consistently deliver reproducible results, significantly enhancing the reliability of our experiments. Thank you, Team Purma Biologics, for your good products and support!
Frequently asked questions
What working concentration should I use for Eukaryotic Cell Culture Suited Antibiotics?
Most antibiotic mixes are supplied as a 100X stock, giving a 1 in 100 dilution into complete medium. Check the Certificate of Analysis for the specific lot, since concentration varies between formulations.
Should antibiotics be used routinely in cell culture?
Most quality guidance advises against it. Continuous use masks low level contamination, allows poor aseptic technique to go unnoticed, and can select for resistant organisms. They are appropriate for high risk work such as primary tissue rather than as a standing habit.
Will this prevent yeast or fungal contamination?
Not unless it contains an antimycotic. Penicillin and streptomycin act on bacteria only. Preventing fungi or yeast requires amphotericin B or a similar antimycotic, and even then technique matters more than chemistry.
How should Eukaryotic Cell Culture Suited Antibiotics be stored?
Frozen at minus 20 degrees C and aliquoted on first thaw. Activity declines with repeated freeze and thaw cycles, which shows up as contamination creeping back into cultures that were previously clean.
Is it sterility and endotoxin tested?
Yes. Every lot is filtered and tested, and a Certificate of Analysis is issued per lot and available on request.
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