Chemically Defined MEM_alpha_nucleosodes_Final
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SKU: Select options below to see it Categories: Cell Culture Media, MEM α-Modification with Nucleosides in Hanks’ Buffer Tags: Advanced, Advanced MEM Alpha, Cell Culture, Hanks’ Buffer, HBSS, Human Transferrin, L-Alanyl-L-Glutamine, MEM-α, Nucleosides, Recombinant Insulin, Serum Reduction

Chemically Defined MEM- Alpha with Nucleosides in Hanks’ Buffer

PurMa™ Chemically Defined CD MEM Alpha with Nucleosides in Hanks’ Buffer (CD-MEM-α-N /HBSS) medium does not need any serum, fully synthetic and is free of any trace of animal products.  Additionally, the cell growth with CD-MEM-α-N /HBSS Medium is equivalent to Medium+ 10% FBS

SKU Select options below to see it
SKU: N/A Categories: Cell Culture Media, MEM α-Modification with Nucleosides in Hanks’ Buffer Tags: Advanced, Advanced MEM Alpha, Cell Culture, Hanks’ Buffer, HBSS, Human Transferrin, L-Alanyl-L-Glutamine, MEM-α, Nucleosides, Recombinant Insulin, Serum Reduction

Description

Background

PurMa™ Chemically Defined CD MEM Alpha with Nucleosides in Hanks’ Buffer (CD-MEM-α-N /HBSS) is a proprietary formulation used to grow cells in vitro without any undefined or animal-derived components. Moreover, (CD-MEM-α-N /HBSS)  Medium contains known quantities of pure chemicals, including amino acids, vitamins, salts, sugars, and more importantly a proprietary combination of synthetic growth factors.

It is absolutely crucial to mention that the PurMa™ Growth Supplement for each conventional media such as DMEM, IMDM, RPMI-1640… (base media) are slightly different in combination, amount of several growth factors, ions, chemicals, pH with particular adjustments need to be done, based on the environments the base media provides.

This provides a consistent and controlled environment for cell growth and research. Noticeably, (CD-MEM-α-N /HBSS) Medium is essential for applications that require reproducibility and regulatory compliance as well as for studying specific cellular processes without extraneous variables. Furthermore, (CD-MEM-α-N /HBSS) Medium is designed for custom formulations tailored for various cell types

Chemically Defined MEM-Alpha with Nucleosides in Hanks' Buffer

Important Notes

It is crucially vital, for a gradual transition from media containing FBS to chemically defined cell culture media, to follow these steps:

  • Starting Point: 10% FBS+ (control) (you need two controls, one to be left in only 10% FBS and continue monitoring for comparing with 0% FBS + PurMa™ Chemically Defined Medium. The second one chemically defined medium + 10% FBS which is going through the reduction of FBS process)
  • 48 hours later: 8% FBS in + PurMa™ Chemically Defined Medium
  • 48 hours later: 6% FBS in + PurMa™ Chemically Defined Medium
  • 48 hours later: 4% FBS+ PurMa™ Chemically Defined Medium
  • 48 hours later: 2% FBS+ PurMa™ Chemically Defined Medium
  • 48 hours later: 1% FBS + PurMa™ Chemically Defined Medium
  • 24 hours later: 0% FBS and only PurMa™ Chemically Defined Medium

Importantly, for each change, spin down and fully discard the media and replace it with the less percentages of FBS.

Noticeably, you don’t have to start with a huge number of cells (~500,000 cells/ml). Additionally, we strongly recommend you start with more than one plate as some of the plates might get contaminated

So, once the cells are kept in chemically defined Media, you should always keep them in the same chemically defined medium from the same vendor and the same formulation, even for freezing (see below).

Usually, after you get your cells adapted to PurMa™ Chemically Defined Medium you need to make several plates and freeze down several vials. The mentioned cells are adapted to chemically defined medium and when you thaw them in the first week, in addition to PurMa™ Chemically Defined Medium, you need to add 10% PurMa™ CD Booster (Cat# P3C10144).

Formulation

The (CD-MEM-α-N /HBSS) Medium includes a base formulation including L-Alanine -L Glutamine :Base Formulation of Minimum Essential Medium Alpha with Nucleosides in Hanks’ Buffer (CD-MEM-α-N)

Additionally, (CD-MEM-α-N /HBSS) Medium contains a proprietary synthetic cocktail of specific growth factors, hormones, and other nutrients which make it independent of any serum and any trace of animal products.

References

  1. A previously functional tetracycline-regulated transactivator fails to target gene expression to the bone. Schmidt E, Eriksson M. BMC Res Notes. 2011 Aug 11;4:282. doi: 10.1186/1756-0500-4-282. PMID: 21835026
  2. The growth of L-cells and Vero cells on an autoclavable MEM-peptone medium. L Keay Biotechnology and bioengineering, 19(3), 399-411 (1977-03-01).
  3. Minimum essential medium alpha (MEM) enhances assisted reproductive technology results. I. Mouse embryo study. Wun WS, et al. Assist Reprod Genet. 1994 Jul;11(6):303-7. doi: 10.1007/BF02215717. PMID: 7734915.
Parameter Specification
Appearance Red, clear liquid
pH 7.2 ± 0.1
Osmolality 275-360 mOsm/L
Endotoxin NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive Sodium pyruvate
Indicator Phenol red
Mycoplasma Detection Negative
Sterility Tested Sterile filtered using 0.1 µm filter, 3 Times
Form Liquid
Shipping Condition  Room temperature, Overnight

 

Additional information

Condition

CD-MEM-α-N (HBSS) Standard Formulation, CD-MEM-α-N (HBSS) w/o L-Alanyl-L-Glutamine, CD-MEM-α-N (HBSS) w/o Phenol Red, CD-MEM-α-N (HBSS) w/o Sodium Bicarbonate, CD-MEM-α-N (HBSS) w/o Glucose, CD-MEM-α-N (HBSS) w/o Lipoic Acid, CD-MEM-α-N (HBSS) w/o L-Alanyl-L-Glutamine , w/o Phenol Red, CD-MEM-α-N (HBSS) w/o L-Alanyl-L-Glutamine , w/o Phenol Red, w/o Sodium Bicarbonate, CD-MEM-α-N (HBSS) 15 mM HEPES (3.6 g/L), CD-MEM-α-N (HBSS) 25 mM HEPES (5.9 g/L), CD-MEM-α-N (HBSS) w/o Sodium Pyruvate, CD-MEM-α-N (HBSS) High Sodium Bicarbonate (0.85 g/L), CD-MEM-α-N (HBSS) High Glucose (4.5 g/L), CD-MEM-α-N (HBSS) 15 mM HEPES (3.6 g/L), High Glucose (4.5 g/L), CD-MEM-α-N (HBSS) 25 mM HEPES (5.9 g/L), High Glucose (4.5 g/L)
Size

1X 100 ml, 1X 1000 ml, 1X 500 ml

Documents

Certificate of Analysis (COA): Available upon request for your specific order — email info@purmabiologics.com and we will send the current lot documentation.

Safety Data Sheet (SDS): Available upon request — email info@purmabiologics.com.

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I recently had the pleasure of interacting with Dr. Seif Azadi from Purma Biologics, and I cannot thank him enough for his expertise and prompt assistance. His deep knowledge and dedication were instrumental in meeting our laboratory’s needs with remarkable efficiency. We ordered FBS, DMEM, and Trypsin from Purma Biologics, and I’m thrilled to report that all the products have performed exceptionally well. They consistently deliver reproducible results, significantly enhancing the reliability of our experiments. Thank you, Team Purma Biologics, for your good products and support!
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Frequently asked questions

How should I store Chemically Defined MEM- Alpha with Nucleosides in Hanks’ Buffer?

Store at 2 to 8 degrees C, protected from light, and do not freeze unless the label states otherwise. Freezing can precipitate salts and degrade light sensitive components such as riboflavin and folic acid. Always follow the storage line on the Certificate of Analysis for the specific lot.

What is the shelf life once opened?

Unopened and correctly stored, the typical shelf life is 12 months from manufacture. Once opened, use within about four weeks and always handle in a laminar flow hood, since the practical limit after opening is contamination risk rather than chemical stability.

Is it sterility and endotoxin tested?

Yes. Every lot is sterile filtered and tested for sterility and endotoxin, and a Certificate of Analysis is issued for each lot. The COA is available on request for any catalogue number.

Do I need to add L-glutamine?

It depends on the formulation you order. Standard formulations may contain free L-glutamine, which degrades in solution over time, while MAX formulations use a stable L-alanyl-L-glutamine dipeptide that does not require fresh supplementation. Check the option you have selected before adding more.

Can I order this in bulk or as a custom formulation?

Yes. Bulk volumes and custom formulations are made to order, including adjusted glucose, bicarbonate, buffering and cation content. Use the request bulk pricing button on this page and include the specification you need.

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