Basal Medium Eagle (BME) in Earle’s Buffer
Basal Medium Eagle (BME) in Earl’s Buffer developed by Harry Eagle, and certainly is one of the most widely used of all synthetic cell culture media. BME has demonstrated widely applicable for supporting monolayer cell growth in wide variety of normal as well as transformed cell lines.
- ✓Proudly made in the USA, manufactured in house
- ✓Certificate of Analysis available for every lot
- ✓Backed by decades of cell culture and protein biochemistry expertise
- ✓Bulk pricing, purchase orders, and custom formulations welcome
Description
Background
Basal Medium Eagle (BME) in Earle’s Buffer originally developed by Harry Eagle. This media is one of the most widely used of all synthetic cell culture media. Furthermore, Basal Medium Eagle (BME) in Earle’s Buffer, when properly supplemented, has demonstrated wide applicability, for supporting monolayer growth of a wide variety of normal and transformed cell lines. Moreover, PurMa Biologics manufacturers BME with Earle’s as well as in Hanks’ salts for use without CO2.
Ingredients
PurMa™ Basal Medium Eagle (BME) in Earle’s Buffer contains:
Earl’s buffer includes:
- Calcium chloride
- Magnesium sulfate
- Potassium chloride
- Sodium bicarbonate
- Sodium chloride
- and lastly, Sodium Phosphate, monobasic
PurMa Biologics so far, manufactures 109 types of BME in Earle’s buffer.
Why PurMa Biologics? Why we stand behind our cell culture media.
Our products are the outcome of 30 years of experience in cutting-edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. Moreover, we utilize 99% pure amino acids. This will significantly increase the consistency among your experiments. Undoubtedly, considering the highest quality, our prices certainly are extremely reasonable.
Application
Basal Medium Eagle (BME) in Earle’s Buffer provides an excellent nutritional capacity for suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts as well as primary rat astrocytes.
Formulation
for complete formulation click here, alternatively you can find it in “Formulation Tab”: BME with Earle’s Buffer Formulation
References:
- Improved chemically defined basal medium (CMRL-1969) for primary monkey kidney and human diploid cells. Healy GM, Teleki S, von Seefried A, Walton MJ, Macmorine HG. Appl Microbiol. 1971 Jan;21(1):1-5. doi: 10.1128/am.21.1.1-5.1971. PMID:
- Nutritional requirements for the production of herpes simplex virus. I. Influence of glucose and glutamine of herpes simplex virus production by HeLa cells. LEWIS VJ Jr, SCOTT LV. J Bacteriol. 1962 Mar;83(3):475-82. doi: 10.1128/jb.83.3.475-482.1962. PMID: 14464909
| Parameter | Specification |
|---|---|
| Appearance | Red, clear liquid |
| pH | 7.2 ± 0.1 |
| Osmolality | 275-360 mOsm/L |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Suitability | Suitable for mammalian cell culture |
| Additive | N/A |
| Indicator | Phenol red |
| Mycoplasma Detection | Negative |
| Sterility Tested | Sterile filtered using 0.22 µm filter |
| Form | Liquid |
| Shipping Condition | Room temperature |
Additional information
| Condition | BME Standard Formulation, BME w/o Glutamine, BME w/o Phenol Red, BME w/o Sodium Bicarbonate, BME w/o Glucose, BME w/o Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, BME 15 mM HEPES (3.6 g/L), BME 25 mM HEPES (5.9 g/L), BME With Sodium Pyruvate (0.11 g/L), BME Low Sodium Bicarbonate (1.2 g/L), BME High Glucose (4.5 g/L), BME 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.2 g/L), BME 25 mM HEPES (5.9 g/L), Low Sodium Bicarbonate (1.2g/L) |
|---|---|
| Format | Liquid, Powder |
| Size | 10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L |
Documents
Certificate of Analysis (COA): Available upon request for your specific order — email info@purmabiologics.com and we will send the current lot documentation.
Safety Data Sheet (SDS): Available upon request — email info@purmabiologics.com.
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