Advanced Dulbecco’s Modified Eagle Medium/F-12 (A-DMEM F-12)

Advanced Dulbecco’s Modified Eagle Medium/F-12 (A-DMEM F-12) needs 2-5% FBS and provides more consistency.

Description

Advanced Dulbecco′s Modified Eagle′s Medium/F-12 (A-DMEMF-12)

Background:

PurMa Advanced Dulbecco’s Modified Eagle Medium/F-12 (A-DMEM F-12) contains components that allow for a 60–90% reduction in FBS supplementation. More importantly, using synthetic components such as recombinant insulin, albumin, and transferrin increase consistency. This results in less variation amongst lot-to-lot serum changes.  In Advanced DMEM F-12, L-Alanyl-L-Glutamine is used as the source of glutamine.  Additionally, reducing FBS requirements lowers the cost of cell culture experiments.

A-DMEM F-12 Contains:

  • Advanced Dulbecco’s Modified Eagle Medium/F-12 (A-DMEM F-12) contains all the twenty essential and non-essential amino acids with higher concentrations than regular and MAX formulations.
  • It includes the elements which significantly reduce the need for a serum for cell culture.

Crucial Ingredients:

  • PurMaTM Human Transferrin (Holo): prevents improper iron storage and delivery in cell culture systems which is a major contributor to oxidative stress and protein damage.
  • PurMaTM Insulin Recombinant
  • Trace of other elements which mimic the elements in fetal bovine serum.

Additionally, A-DMEM F-12 is a significant step toward a chemically defined form of this media. Undoubtedly, Chemically Defined DMEM F-12 (CD-DMEMF-12) is the outcome of decades of cutting-edge research. CD-DMEM F-12 will soon be offered globally to laboratories who are looking for consistency and repeatability among their data.

Formulation: Complete formulation is available here: Advanced DMEM-F12 Formulation

References:

  1.  Clonal growth of mammalian cells in a chemically defined, synthetic medium . DULBECCO et al. 1959 Jul;8(3):396-7. doi: 10.1016 /0042-68 22(59)90043-1. PMID: 13669362.
  2. Plaque production by the polyoma virus. DULBECCO et al. 1959 Jul;8(3):396-7. doi: 10.1016/0042-6822(59)90043-1. PMID: 1366936.
  3. Evaluation of a simple protein free medium that supports high levels of monoclonal antibody production. Qi et al. 1996 Jun;21(2):95-109. doi: 10.1007/BF02215660. PMID: 22358660.
Parameter Specification
Appearance Red, clear liquid
pH 7.2 ± 0.1
Osmolality 275-360 mOsm/L
Endotoxin NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive Sodium pyruvate
Indicator Phenol red
Mycoplasma Detection Negative
Sterility Tested Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

 

Additional information

Condition

A-DMEM F-12 Standard Formulation, A-DMEM F-12 w/o L-Alanyl-L-Glutamine., A-DMEM F-12 w/o phenol Red, A-DMEM F-12 w/o Sodium Bicarbonate, A-DMEM F-12 w/o Sodium Pyruvate, A-DMEM F-12 w/o Glucose, A-DMEM F-12 w/o Lipoic Acid, A-DMEM F-12 w/o L-Alanyl-L-Glutamine, w/o phenol Red, A-DMEM F-12 w/o Phenol Red, w/o Sodium Bicarbonate, A-DMEM F-12 w/o Sodium Bicarbonate, w/o Sodium Pyruvate, A-DMEM F-12 w/o L-Alanyl-L-Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, A-DMEM F-12 w/o Phenol Red, w/o Sodium Bicarbonate, w/o Sodium Pyruvate, A-DMEM F-12 w/o L-Alanyl-L-Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, w/o Sodium Pyruvate, A-DMEM F-12 Low Glucose (1.5 g/L), A-DMEM F-12 Low Sodium Bicarbonate (1.2 g/L), A-DMEM F-12 15 mM HEPES (3.6 g/L), A-DMEM F-12 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.2 g/L, A-DMEM F-12 w/o Sodium Pyruvate, w/o phenol Red, A-DMEM F-12 w/o Phenol Red, 15 mM HEPES (3.6 g/L), A-DMEM F-12 w/o Sodium Pyruvate, with 15 mM HEPES (3.6 g/L), A-DMEM F-12 w/o Phenol Red, Low Sodium Bicarbonate (1.2 g/L), A-DMEM F-12 w/o Sodium Pyruvate, Low Sodium Bicarbonate (1.2 g/L), A-DMEM F-12 w/o Sodium Pyruvate, w/o Phenol Red, with 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.2 g/L), A-DMEM F-12 w/o Phenol Red, w/o Sodium Pyruvate, with 15 mM HEPES (3.6 g/L), A-DMEM F-12 w/o Phenol Red, with 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.2 g/L), A-DMEM F-12 w/o Sodium Pyruvate, with 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.2 g/L), A-DMEM F-12 High Glucose (4.5 g/L)

Format

Liquid, Powder

Size

10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed

Quality Control

[vc-quality-control-tab]

Formulation

Advanced DMEM F-12 Formulation 

 

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