Advanced Kaighn’s Modification of Ham’s F-12 (A-Ham’s F-12K)

Advanced Kaighn’s Modification of Ham’s F-12 Medium (A-Ham’s F-12K) contains L-Alanyl-L-Glutamine as well as ingredients to allow for serum reduction.  Additionally, A-Ham’s F-12K needs 2-5% FBS and provides more consistency.

Description

Advanced Kaighn’s Modification of Ham’s F-12 (A-Ham’s F-12K)

Background

PurMaTM Advanced Kaighn’s Modification of Ham’s F-12 Medium (A-Ham’s F-12K) contains components that allow for a 60–90% reduction in FBS supplementation. More importantly, using synthetic components such as recombinant insulin, albumin, and transferrin increase consistency. This results in less variation amongst lot-to-lot serum changes. In A-Ham’s F-12K, L-Alanyl-L-Glutamine is used as the source of glutamine. Additionally, reducing FBS requirements lowers the cost of cell culture experiments.

Contents

Advanced Kaighn’s Modification of Ham’s F-12 Medium (A-Ham’s F-12K) Contains:

  • includes all twenty essential and non-essential amino acids with higher concentrations than regular and MAX formulations.
  • A-Ham’s F-12K incorporates elements that significantly reduce the need for a serum for cell culture.

Crucial Ingredients:

  • PurMaTM Human Transferrin (Holo): prevents Improper iron storage and delivery in cell culture systems which is a major contributor to oxidative stress and protein damage.
  • PurMaTM Insulin Recombinant
  • Trace of other elements which mimic the elements in fetal bovine serum.

Consequently, Advanced Kaighn’s Modification of Ham’s F-12 Medium (A-Ham’s F-12K) is a significant step toward a chemically defined form of this media. Unquestionably, Chemically Defined Advanced Ham’s F-12K (CD-A-Ham’s F-12K) is the outcome of decades of cutting-edge research and will soon be offered globally to laboratories that are looking for consistency and repeatability in their data.

Formulation

Complete formulation is available here: Advanced Ham’s-F12 Formulation

References

Parameter Specification
Appearance Red, clear liquid
pH  7.2 ± 0.1
Osmolality  275-360 mOsm/L
Endotoxin  NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive  Sodium pyruvate
Indicator  Phenol red
Mycoplasma Detection Negative
Sterility Tested  Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition Room temperature

 

Additional information

Condition

A-HAM’S F-12K Standard Formulation, A-HAM’S F-12K w/o L-Alanyl-L-Glutamine, A-HAM’S F-12K w/o Phenol Red, A-HAM’S F-12K w/o Sodium Bicarbonate, A-HAM’S F-12K w/o Sodium Pyruvate, A-HAM’S F-12K w/o Glucose, A-HAM’S F-12K w/o Lipoic Acid, A-HAM’S F-12K w/o L-Alanyl-L-Glutamine, w/o Phenol Red, A-HAM’S F-12K w/o Phenol Red, w/o Sodium Bicarbonate, A-HAM’S F-12K w/o Sodium Bicarbonate, w/o Sodium Pyruvate, A-HAM’S F-12K w/o L-Alanyl-L-Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, A-HAM’S F-12K w/o Phenol Red, w/o Sodium Bicarbonate, w/o Sodium Pyruvate, A-HAM’S F-12K w/o L-Alanyl-L-Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, w/o Sodium Pyruvate, A-HAM’S F-12K High Glucose (4.5 g/L), A-HAM’S F-12K Low Sodium Bicarbonate (1.5 g/L), A-HAM’S F-12K High Sodium Bicarbonate (3.7 g/L), A-HAM’S F-12K 15 mM HEPES (3.6 g/L), A-HAM’S F-12K 25 mM HEPES (5.9 g/L), A-HAM’S F-12K 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.5 g/L), A-HAM’S F-12K 25 mM HEPES (5.9 g/L), Low Sodium Bicarbonate (1.5 g/L), A-HAM’S F-12K w/o Sodium Pyruvate, w/o Phenol Red, A-HAM’S F-12K w/o Phenol Red, with 15 mM HEPES (3.6 g/L), A-HAM’S F-12K w/o Phenol Red, with 25 mM HEPES (5.9 g/L), A-HAM’S F-12K w/o Sodium Pyruvate, with 15 mM HEPES (3.6 g/L), A-HAM’S F-12K w/o Sodium Pyruvate, with 25 mM HEPES (5.9 g/L), A-HAM’S F-12K w/o Phenol Red, Low Sodium Bicarbonate (1.5 g/L), A-HAM’S F-12K w/o Sodium Pyruvate, Low Sodium Bicarbonate (1.5 g/L), A-HAM’S F-12K w/o Sodium Pyruvate, w/o Phenol Red, with 15 mM HEPES (3.6 g/L) Low Sodium Bicarbonate (1.5 g/L), A-HAM’S F-12K w/o Sodium Pyruvate, w/o Phenol Red, with 25 mM HEPES (5.9 g/L) Low Sodium Bicarbonate (1.5 g/L), A-HAM’S F-12K w/o Phenol Red, w/o Sodium Pyruvate, with 15 mM HEPES (3.6 g/L), A-HAM’S F-12K w/o Phenol Red, w/o Sodium Pyruvate, with 25 mM HEPES (5.9 g/L), A-HAM’S F-12K w/o Phenol Red, with 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.5 g/L), A-HAM’S F-12K w/o Phenol Red, with 25 mM HEPES (5.9 g/L), Low Sodium Bicarbonate (1.5 g/L), A-HAM’S F-12K w/o Sodium Pyruvate, with 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.5 g/L), A-HAM’S F-12K w/o Sodium Pyruvate, with 25 mM HEPES (5.9 g/L), Low Sodium Bicarbonate (1.5 g/L)

Format

Liquid, Powder

Size

10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed

Quality Control

[vc-quality-control-tab]

Formulation

Advanced Ham’s F-12K

SDS

SDS

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