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Ham's F-12K L‑Alanyl‑L‑Glutamine

PurMa™ Kaighn’s Modification of Ham’s F-12-MAX (Ham’s F-12K-MAX)

(43 customer reviews)

PurMa™ F-12K-MAX, an advanced formulation of Ham’s F-12K L‑Alanyl‑L‑Glutamine, enhances the traditional Kaighn’s F-12 medium by incorporating 4 mM L‑alanyl‑L‑glutamine. This modification ensures superior stability and significantly reduces ammonia formation while improving the medium’s buffering capacity. Additionally, PurMa™ F-12K-MAX offers an extended shelf life and is specifically optimized to support primary and sensitive mammalian cell cultures effectively.

SKU: N/A Categories: Cell Culture Media, Kaighn's Modification of Ham's F-12K Medium Tags: Cell Culture, glucose, Ham's F-12, Ham's F-12K, Kaighn's, L-Alanyl-L-Glutamine, Mammalian Cells, MAX, Phenol Red, Without Phenol Red

Description

PurMa™ Kaighn’s Modification of Ham’s F-12-MAX (Ham’s F-12K-MAX)

Product Description

Ham’s F-12K L‑Alanyl‑L‑Glutamine (F‑12K‑MAX) significantly enhances cell culture performance by offering superior stability compared to free L‑glutamine, which extends shelf life and mitigates decomposition. Its slower release profile effectively reduces ammonia accumulation, fostering higher cell viability, particularly in sensitive primary cell lines. Additionally, the formulation provides enhanced buffering capacity, ensuring pH stability amidst routine culture disturbances. These attributes make Ham’s F-12K L‑Alanyl‑L‑Glutamine an invaluable resource for optimizing the growth conditions of primary hepatocytes, oocytes, and various other cell lines.

Key benefits of Ham’s F‑12K L‑Alanyl‑L‑Glutamine (F‑12K‑MAX)

  • Stability and shelf life:Ham’s F-12K L‑Alanyl‑L‑Glutamine is far more stable than free L‑glutamine in solution, extending storage life and reducing decomposition during handling and incubation.
  • Moreover, Reduced ammonia and improved culture health: slower release of glutamine minimizes ammonia build‑up, supporting higher viability and more consistent growth, especially for sensitive primary cells.
  • Additionally, buffering capacity:Ham’s F-12K L‑Alanyl‑L‑Glutamine features increased pH buffering (e.g., selectable NaHCO3/HEPES options) for improved pH stability during culture and after routine disturbances.
  • Performance of Ham’s F-12K L‑Alanyl‑L‑Glutamine for primary and sensitive lines: optimized for primary hepatocytes, oocytes, and a broad range of cell lines that perform better with stable glutamine sources and robust buffering.
  • Practical handling: improved thermal and storage stability with F12k with l alanyl lglutamineenhanced reduces waste and logistics burden in labs and production settings.

Typical composition highlights (F‑12K‑MAX)

  • L‑Alanyl‑L‑Glutamine: 4 mM (≈0.869 g/L)
  • Sodium pyruvate: 5 mM
  • High glucose option: 4.5 g/L (select formulations available)
  • Sodium bicarbonate: standard 3.7 g/L (other NaHCO3 levels available to match incubator CO2)
  • Non‑essential amino acids, phenol red; no proteins or growth factors (supplement per protocol, e.g., 10% FBS)

Mechanism & rationale Free L‑glutamine degrades in aqueous solutions to yield ammonia and breakdown products that can impair cell health and assay reproducibility. L‑alanyl‑L‑glutamine, a dipeptide, hydrolyzes more slowly, providing a steady supply of glutamine and alanine to cells while minimizing toxic ammonia accumulation. The result is improved culture stability, reproducible performance, and fewer lot losses due to media degradation.

Recommended use

  • Use hams f12k with lalanyllglutamine (F‑12K‑MAX )as a basal medium and supplement per your cell type (serum, growth factors, or defined supplements).
  • Choose the NaHCO3/HEPES variant matching your incubator CO2 setting for best pH stability.
  • Store unopened at 2–8 °C; avoid repeated freeze–thaw. Validate for your specific cell lines and assays.

Why PurMa F‑12K‑MAX

With 30+ years of cell culture formulation expertise and high‑purity (>99%) amino acids, PurMa Biologics manufactures hams f12k with l alanyl l Glutamine (F‑12K‑MAX )under rigorous QC to ensure reproducibility and lot consistency. Our extended portfolio and technical support help labs choose the right NaHCO3/HEPES options and supplementation strategy.

FAQs (use Rank Math FAQ schema) Q: What is Ham’s F‑12K L‑Alanyl‑L‑Glutamine (F‑12K‑MAX)?
A: Ham’s F‑12K L‑Alanyl‑L‑Glutamine (F‑12K‑MAX) is PurMa’s Kaighn’s modification of Ham’s F‑12 medium formulated with 4 mM L‑alanyl‑L‑glutamine for enhanced media stability, reduced ammonia, and improved culture performance.

FAQS

Q: How does L‑alanyl‑L‑glutamine compare to L‑glutamine?
A: L‑alanyl‑L‑glutamine is a stable dipeptide that degrades much more slowly than free L‑glutamine, producing less ammonia and providing a steadier glutamine supply—this improves shelf life and culture reproducibility.

Q: Which cells benefit most from F‑12K‑MAX?
A: Primary hepatocytes, oocytes, sensitive primary cultures, and many cell lines that are sensitive to ammonia or require consistent glutamine supply typically show improved viability and growth in F‑12K‑MAX.

Q: Do I need to supplement Ham’s F-12K L‑Alanyl‑L‑Glutamine  (F‑12K‑MAX)?
A: F‑12K‑MAX contains no proteins or growth factors and typically requires supplementation (e.g., 5–10% FBS or defined supplements) based on your cell type and protocol.

Q: How should I store and handle F‑12K‑MAX?
A: Store unopened bottles at 2–8 °C, protect from light, and avoid repeated freeze–thaw cycles. Prepare working volumes aseptically and validate performance for your assays.

Formulation the Ham’s F-12K L‑Alanyl‑L‑Glutamine , Kaighn’s modification of Ham’s F-12-MAX, designated as Ham’s F-12K-MAX, introduces significant enhancements to the original formulation. This updated version aims to optimize performance while maintaining the essential characteristics of the initial design. Through meticulous adjustments in composition and process parameters, the modified formulation demonstrates improved efficacy and stability. As a result, it offers a more reliable solution for applications requiring heightened precision and performance. F12k universal complete growth medium is proven to be one of the best media for growing primary cells

For complete formulation of PurMa™ Ham’s F-12K-MAX, click here: PurMa™ Ham F12K-MAX Formulation

References

  1.  Clonal growth of mammalian cells in a chemically defined, synthetic medium . DULBECCO R, FREEMAN G. 1959 Jul;8(3):396-7. doi: 10.1016/0042-6822(59)90043-1. PMID: 13669362.
  2. Plaque production by the polyoma virus. DULBECCO R, FREEMAN G. 1959 Jul;8(3):396-7. doi: 10.1016/0042-6822(59)90043-1. PMID: 13669362.
  3. The nucleic acid of polyoma virus. Smith, J.D., Freeman, G., Vogt, M., et al., (1960) The nucleic acid of polyoma virus. Virology 12:185
Parameter Specification
Appearance Red, clear liquid
pH  7.2 ± 0.1
Osmolality  275-360 mOsm/L
Endotoxin  NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive  Sodium pyruvate
Indicator  Phenol red
Mycoplasma Detection Negative
Sterility Tested  Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

Additional information

Condition

HAM’S F-12K- MAX, Standard Formulation, HAM’S F-12K- MAX, Standard Formulation, ATCC Formulation, HAM’S F-12K- MAX, w/o Phenol Red, HAM’S F-12K- MAX, w/o Sodium Bicarbonate, HAM’S F-12K- MAX, w/o Sodium Pyruvate, HAM’S F-12K- MAX, w/o Glucose, HAM’S F-12K- MAX, w/o Lipoic Acid, HAM’S F-12K- MAX, w/o Phenol Red, w/o Sodium Bicarbonate, HAM’S F-12K- MAX, w/o Sodium Bicarbonate, w/o Sodium Pyruvate, HAM’S F-12K- MAX, High Glucose (4.5 g/L), HAM’S F-12K- MAX, Low Sodium Bicarbonate (1.5 g/L), HAM’S F-12K- MAX, High Sodium Bicarbonate (3.7 g/L), HAM’S F-12K- MAX, 15 mM HEPES (3.6 g/L), HAM’S F-12K- MAX, 25 mM HEPES (5.9 g/L), HAM’S F-12K- MAX, 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.5 g/L), HAM’S F-12K- MAX, 25 mM HEPES (5.9 g/L), Low Sodium Bicarbonate (1.5 g/L), HAM’S F-12K- MAX, w/o Sodium Pyruvate, w/o Phenol Red, HAM’S F-12K- MAX, w/o Phenol Red, with 15 mM HEPES (3.6 g/L), HAM’S F-12K- MAX, w/o Phenol Red, with 25 mM HEPES (5.9 g/L), HAM’S F-12K- MAX, w/o Phenol Red, Low Sodium Bicarbonate (1.5 g/L), HAM’S F-12K- MAX, w/o Sodium Pyruvate, Low Sodium Bicarbonate (1.5 g/L)
Format

Liquid, Powder
Size

10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L

43 reviews for PurMa™ Kaighn’s Modification of Ham’s F-12-MAX (Ham’s F-12K-MAX)

  1. azadi@live.ca –

    Review: we have purchased Lipid Depleted FBS , DMEM F12 …. they were good quality and decent price
    Have you purchased: Yes
    Time of submission: 29-Jan-2025 23:10:30
    Ease of placing the order: 5/5

  2. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 21-Feb-2025 15:30:00
    Ease of placing the order: ‘not given’

  3. Gregory-Mullen@ouhsc.edu –

    Review: We buy *all* of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
    Have you purchased: Yes
    Time of submission: 21-Feb-2025 15:46:22
    Ease of placing the order: 5/5

  4. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 24-Feb-2025 13:50:14
    Ease of placing the order: ‘not given’

  5. lrothblu@ouhsc.edu –

    Review: Serum worked beautifully. Cells grew well and had their usual cycle time. Tissue culture medium also worked very well.
    Have you purchased: It is my first Purchase
    Time of submission: 24-Feb-2025 13:53:22
    Ease of placing the order: 5/5

  6. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 27-Feb-2025 17:42:44
    Ease of placing the order: ‘not given’

  7. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 05-Mar-2025 16:31:44
    Ease of placing the order: ‘not given’

  8. michael-rudolph@ouhsc.edu –

    Review: I have worked directly with PurMa biologics on a number of projects ongoing in my lab. I find the media and the specialty Lipid Depleted Serum to be high quality and very consistent among batches, which is critical to our adipogenesis projects using primary stem cells. Also, the service from PurMa is outstanding for my lab.
    Have you purchased: Yes
    Time of submission: 05-Mar-2025 16:35:21
    Ease of placing the order: 5/5

  9. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 05-Mar-2025 17:29:13
    Ease of placing the order: ‘not given’

  10. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 10-Mar-2025 14:32:38
    Ease of placing the order: ‘not given’

  11. jkurien@hausbio.com –

    Review: We have been working with PurMa biologics for the past several years , we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum . We appreciate the high quality and reasonable price, and great customer service.
    Have you purchased: Yes
    Time of submission: 12-Mar-2025 12:34:09
    Ease of placing the order: 5/5

  12. hyeungk1968@gmail.comthe –

    Review: I used Lipid Free horse serum of PurMa Biologics.
    The quality is great!
    Have you purchased: Yes
    Time of submission: 17-Mar-2025 12:09:58
    Ease of placing the order: 5/5

  13. jaya-krishnan@omrf.org –

    Review: I started my lab using the regular Gibco media components. We have shifted to Purma past 6 months and see no difference in the quality of our cell culture. Simply saying that Purma is cheaper, quicker to deliver and as good as Gibco. The best part was that Purma customized several media for our fish cell lines which was really helpful. Lastly, Seif walked through the customization of media over Zoom, that gave a lot of confidence for using those media solutions.
    Have you purchased: Yes
    Time of submission: 17-Mar-2025 14:22:58
    Ease of placing the order: 4/5

  14. tayyab-afzal@omrf.org –

    Review: We bought Premium FBS and DMEM cell culture media from PurMa Biologics. We are using them for several mammalian cell line and primary cell culture and the cell growth is perfectly fine. We are totally satisfied with the product quality. Importantly as local vendor, we can have the product available as per our demand ASAP.
    Have you purchased: Yes
    Time of submission: 18-Mar-2025 10:01:50
    Ease of placing the order: 5/5

  15. Fitzkr@gmail.com –

    Review: We purchase everything we need related to cell and tissue culture from PurMa, reagents like: DMEM, LL15, EMEM, FBS, trypsin, antibiotics mix100X and…
    Always great quality, fast processing and shipping.
    We are totally satisfied.
    Have you purchased: Yes
    Time of submission: 22-Mar-2025 10:47:00
    Ease of placing the order: 5/5

  16. vmadka@ouhsc.edu –

    Review:
    Have you purchased: Yes
    Time of submission: 24-Mar-2025 15:02:40
    Ease of placing the order: 4/5

  17. Anupam-Mandal@ouhsc.edu –

    Review: Excellent
    Have you purchased: Yes
    Time of submission: 24-Mar-2025 15:05:43
    Ease of placing the order: 5/5

  18. rohan-varshney@ouhsc.edu –

    Review: Reliable and dependable products. We purchased lipid-depleted FBS (LD-FBS) from PurMa Biologics and it worked great. We cross-checked the lipid content in the serum by GC-MS and Triglyceride assay and as advertised, did not find any lipids in the FBS.
    Have you purchased: Yes
    Time of submission: 24-Mar-2025 15:06:38
    Ease of placing the order: 5/5

  19. Surendra-Shukla@ouhsc.edu –

    Review: Very fast, efficient, and economic service. Great for new PIs like me.
    Have you purchased: Yes
    Time of submission: 25-Apr-2025 14:00:04
    Ease of placing the order: 5/5

  20. vmadka@ouhsc.edu –

    Review:
    Have you purchased: Yes
    Time of submission: 24-Mar-2025 15:02:40
    Ease of placing the order: 4/5

  21. Anupam-Mandal@ouhsc.edu –

    Review: Excellent
    Have you purchased: Yes
    Time of submission: 24-Mar-2025 15:05:43
    Ease of placing the order: 5/5

  22. anil-singh@ouhsc.edu –

    Review: NA
    Have you purchased: Yes
    Time of submission: 25-Mar-2025 10:11:14
    Ease of placing the order: 5/5

  23. Surendra-Shukla@ouhsc.edu –

    Review: Best quality products, best price.
    Have you purchased: Yes
    Time of submission: 18-Apr-2025 09:51:03
    Ease of placing the order: 5/5

  24. madhusmita-rout@ouhsc.edu –

    Review:
    Have you purchased: Yes
    Time of submission: 21-Apr-2025 11:15:46
    Ease of placing the order: 5/5

  25. alexholloway@mesogen.com –

    Review: This serum appears to be a high quality product and worked well for our needs. Purma has an extensive catalog for cell culturing supplies which we are glad we came across.
    Have you purchased: It is my first Purchase
    Time of submission: 24-Apr-2025 16:54:29
    Ease of placing the order: 5/5

  26. jkurien@hausbio.com –

    Review: We have been working with PurMa biologics for the past several years , we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum . We appreciate the high quality and reasonable price, and great customer service.
    Have you purchased: Yes
    Time of submission: 12-Mar-2025 12:34:09
    Ease of placing the order: 5/5

  27. lrothblu@ouhsc.edu –

    Review: Serum worked beautifully. Cells grew well and had their usual cycle time. Tissue culture medium also worked very well.
    Have you purchased: It is my first Purchase
    Time of submission: 24-Feb-2025 13:53:22
    Ease of placing the order: 5/5

  28. hyeungk1968@gmail.comthe –

    Review: I used Lipid Free horse serum of PurMa Biologics.
    The quality is great!
    Have you purchased: Yes
    Time of submission: 17-Mar-2025 12:09:58
    Ease of placing the order: 5/5

  29. jaya-krishnan@omrf.org –

    Review: I started my lab using the regular Gibco media components. We have shifted to Purma past 6 months and see no difference in the quality of our cell culture. Simply saying that Purma is cheaper, quicker to deliver and as good as Gibco. The best part was that Purma customized several media for our fish cell lines which was really helpful. Lastly, Seif walked through the customization of media over Zoom, that gave a lot of confidence for using those media solutions.
    Have you purchased: Yes
    Time of submission: 17-Mar-2025 14:22:58
    Ease of placing the order: 4/5

  30. michael-rudolph@ouhsc.edu –

    Review: I have worked directly with PurMa biologics on a number of projects ongoing in my lab. I find the media and the specialty Lipid Depleted Serum to be high quality and very consistent among batches, which is critical to our adipogenesis projects using primary stem cells. Also, the service from PurMa is outstanding for my lab.
    Have you purchased: Yes
    Time of submission: 05-Mar-2025 16:35:21
    Ease of placing the order: 5/5

  31. tayyab-afzal@omrf.org –

    Review: We bought Premium FBS and DMEM cell culture media from PurMa Biologics. We are using them for several mammalian cell line and primary cell culture and the cell growth is perfectly fine. We are totally satisfied with the product quality. Importantly as local vendor, we can have the product available as per our demand ASAP.
    Have you purchased: Yes
    Time of submission: 18-Mar-2025 10:01:50
    Ease of placing the order: 5/5

  32. Fitzkr@gmail.com –

    Review: We purchase everything we need related to cell and tissue culture from PurMa, reagents like: DMEM, LL15, EMEM, FBS, trypsin, antibiotics mix100X and…
    Always great quality, fast processing and shipping.
    We are totally satisfied.
    Have you purchased: Yes
    Time of submission: 22-Mar-2025 10:47:00
    Ease of placing the order: 5/5

  33. Gregory-Mullen@ouhsc.edu –

    Review: We buy *all* of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
    Have you purchased: Yes
    Time of submission: 21-Feb-2025 15:46:22
    Ease of placing the order: 5/5

  34. azadi@live.ca –

    Review: we have purchased Lipid Depleted FBS , DMEM F12 …. they were good quality and decent price
    Have you purchased: Yes
    Time of submission: 29-Jan-2025 23:10:30
    Ease of placing the order: 5/5

  35. Ravi-Gor@ouhsc.edu –

    Review: I recently had the pleasure of interacting with Dr. Seif Azadi, and I want to express my gratitude for his expertise and timely assistance. His knowledge and dedication were instrumental in addressing our lab’s needs efficiently.
    Have you purchased: It is my first Purchase
    Time of submission: 25-Jun-2025 12:00:09
    Ease of placing the order: 5/5

  36. xiangpeng-li@ou.edu –

    Review: PurMa helped us with the customized medium. They are professional and friendly. I appreciate their great work.
    Have you purchased: Yes
    Time of submission: 14-Aug-2025 12:59:31
    Ease of placing the order: 5/5

  37. jianhua.song@appliedstemcell.com –

    Review: it is reliable vendor!
    Have you purchased: Yes
    Time of submission: 07-Oct-2025 17:14:12
    Ease of placing the order: 5/5

  38. Zhenghui.Liu@appliedstemcell.com –

    Review: We always buy our agarose from PurMa and have never been disappointed.
    Have you purchased: Yes
    Time of submission: 08-Oct-2025 11:17:53
    Ease of placing the order: 5/5

  39. ravi-gor@ou.edu –

    Review: I recently had the pleasure of interacting with Dr. Seif Azadi from Purma Biologics, and I cannot thank him enough for his expertise and prompt assistance. His deep knowledge and dedication were instrumental in meeting our laboratory’s needs with remarkable efficiency.
    We ordered FBS, DMEM, and Trypsin from Purma Biologics, and I’m thrilled to report that all the products have performed exceptionally well. They consistently deliver reproducible results, significantly enhancing the reliability of our experiments.
    Thank you, Team Purma Biologics, for your good products and support!
    Have you purchased: It is my first Purchase
    Time of submission: 23-Oct-2025 17:30:25
    Ease of placing the order: 5/5

  40. info@takbiotechs.com –

    Review: We are extremely satisfied with the quality and consistency of PURMA Biologics products. Both the FBS and cell culture media have delivered excellent performance in our applications, with reliable batch-to-batch consistency. Highly recommended for research and bioprocessing use.
    Have you purchased: Yes
    Time of submission: 11-Apr-2026 14:49:27
    Ease of placing the order: 5/5

  41. aslan.batur@aytakbiotech.com –

    Review: We’ve had a great experience with PURMA Biologics products. In particular, their FBS has shown excellent consistency and performance, and their DMEM media has worked very well in our cell culture applications. Overall, very reliable quality.
    Have you purchased: Yes
    Time of submission: 11-Apr-2026 14:57:39
    Ease of placing the order: 5/5

  42. maya.hart@takbiomed.com –

    Review: It’s been a pleasure working with PURMA Biologics. Their service is efficient, delivery is fast, and the quality of their products has met our expectations consistently. Very smooth collaboration overall.
    Have you purchased: Yes
    Time of submission: 11-Apr-2026 15:06:47
    Ease of placing the order: 5/5

  43. hajipour@etsu.edu –

    Review: PurMa products are of excellent quality and highly reliable.
    Have you purchased: Yes
    Time of submission: 11-Apr-2026 16:23:59
    Ease of placing the order: 5/5

Only logged in customers who have purchased this product may leave a review.

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