Dulbecco’s Modified Eagle’s Medium (DMEM)

Dulbecco’s Modified Eagle’s Medium is a modification of Basal Medium Eagle. Our DMEM, optimized to deliver exceptional growth and consistency, is available in Standard, MAX, and Advanced formulations.  Furthermore, PurMa Biologics currently manufactures 145 conditions of this media.

Description

DMEM

Background; Dulbecco’s Modified Eagle’s Medium

Dulbecco’s Modified Eagle’s Medium (DMEM) is a modification of Basal Medium Eagle (BME). Currently, DMEM is one of the most widely used media.

Major modifications compared to the original formulation:

  1. Although the original Dulbecco’s Modified Eagle’s Medium contained 1000 mg/L of glucose, there are different Glucose amounts used.  As shown below, PurMa Biologics DMEM contains 4.5 g/L glucose. Additionally, PurMa Biologics offers a (1.5 g/L) low glucose (P3SO28105) condition.
  2. Furthermore, DMEM contains fourfold more amino acids and vitamins compared to the original formulation. DMEM is a good choice for growing the primary cell culture of mouse and chicken cells. Moreover, for a variety of normal and transformed cells, this media can be used properly. Each of these DMEMs, by comparison, offers a different combination of L-glutamine and sodium pyruvate.
  3. More importantly, DMEM has several fold more buffering capacities (3.7g/L NaHCO3) Need of FBS.

Lastly, DMEM Contains:

Furthermore, DMEM contains no proteins, lipids, or growth factors. Therefore, it requires supplementation, commonly with 10% Fetal Bovine Serum (FBS).

  • High glucose (4.5g/L)
  • Non-essential amino acids
  • Sodium pyruvate (0.11 g/L)
  • Phenol red (0.0015g/L)
  • L-glutamine (0.585 g/L)

Types of DMEM

Currently, PurMa Biologics Manufactures three forms of DMEM: Standard, MAX, and Advanced. Moreover, the optimization of these three forms of DMEM is the outcome of decades of cell culture research and experience.  Undoubtedly, our DMEM delivers exceptional growth and consistency for your cell culture projects.

Formulation:

Complete formulation is available here: DMEM Formulation

Reference:

  1. The viability change of pigskin in vitro. Ge L, et al. 2010 Jun;36(4):533-8.
  2. A protocol for isolation and culture of mesenchymal stem cells from mouse bone marrow. Soleimani M, Nadri S.Nat Protoc. 2009;4(1):102-6.
Parameter Specification
Appearance Red, clear liquid
pH 7.2 ± 0.1
Osmolality 275-360 mOsm/L
Endotoxin NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive Sodium pyruvate
Indicator Phenol red
Mycoplasma Detection Negative
Sterility Tested Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

 

Additional information

Condition

DMEM- Standard Formulation, DMEM- Without Glutamine, DMEM- Without Phenol Red, DMEM- Without Sodium Bicarbonate, DMEM- With Sodium Pyruvate, DMEM- Without Glucose, DMEM- With Lipoic Acid, DMEM- Without Glutamine, Without Phenol Red, DMEM- Without Glutamine, Without Sodium Bicarbonate, DMEM- Without Glutamine, With Sodium Pyruvate, DMEM- Without Glutamine, Without Phenol Red, Without Sodium Bicarbonate, DMEM- Without Glutamine, Without Phenol Red, With Sodium Pyruvate, DMEM- Without Sodium Bicarbonate With Sodium Pyruvate, DMEM- Low Glucose (1.5 g/L), DMEM- Low Sodium Bicarbonate (1.5 g/L), DMEM- 25 mM HEPES (5.9 g/L), DMEM- 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.5 g/L), DMEM- 25 mM HEPES (5.9 g/L), Low Sodium Bicarbonate (1.5 g/L), DMEM- Without Phenol Red, With Sodium Pyruvate, DMEM- Without Phenol Red, Without Sodium Bicarbonate, DMEM- Without Sodium Bicarbonate, With Sodium Pyruvate, DMEM- With Sodium Pyruvate, 15 mM HEPES (3.6 g/L), DMEM- With Sodium Pyruvate, 25 mM HEPES (5.9 g/L), DMEM- Without phenol Red, 15 mM HEPES (3.6 g/L), DMEM- Without phenol Red, 25 mM HEPES (5.9 g/L), DMEM- Without phenol Red, Low Sodium Bicarbonate (1.5 g/L), DMEM- With Sodium Pyruvate, Low Sodium Bicarbonate (1.5 g/L), DMEM- Without Phenol Red, With Sodium Pyruvate, 15 mM HEPES (3.6 g/L), DMEM- Without Phenol Red, With Sodium Pyruvate, 25 mM HEPES (5.9 g/L), DMEM- Without Phenol Red, With Sodium Pyruvate, 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.5 g/L), DMEM- Without Phenol Red, With Sodium Pyruvate, 25 mM HEPES (5.9 g/L), Low Sodium Bicarbonate (1.5 g/L), DMEM- With Sodium Pyruvate, 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.5 g/L), DMEM- With Sodium Pyruvate, 25 mM HEPES (5.9 g/L), Low Sodium Bicarbonate (1.5 g/L), DMEM- Without Phenol Red, 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.5 g/L), DMEM- Without Phenol Red, 25 mM HEPES (5.9 g/L), Low Sodium Bicarbonate (1.5 g/L)

Format

Liquid, Powder

Size

10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed

Quality Control

[vc-quality-control-tab]

Formulation

DMEM Formulation

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