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PurMa MEM Alpha with Nucleosides MAX in Hanks' Buffer
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SKU: Select options below to see it Categories: Cell Culture Media, MEM α-Modification with Nucleosides in Hanks’ Buffer Tags: Cell Culture, glucose, Hanks’ Buffer, HBSS, L-Alanyl-L-Glutamine, Mammalian Cells, MAX, MEM Alpha, MEM-α, Nucleosides, Phenol Red, Without Phenol Red

MEM Alpha-Modification with Nucleosides MAX (MEM-Alpha-N-MAX) in Hanks’ Buffer

(1 customer review)

Price range: $30.74 through $647.87

MEM α-Modification with Nucleosides MAX in Hanks’ Buffer (MEM-α-N-MAX-HBSS) contains L-Alanyl-L-Glutamine, as well as Nucleosides.   Additionally, MEM-α-N-MAX (HBSS) significantly improves cell viability and growth. We manufacture several types of MEM-α-N-MAX based on your requirements.

SKU Select options below to see it

For research use only. Not for human, veterinary or diagnostic use.

Description

Background

MEM Alpha-Modification with Nucleosides MAX (MEM-Alpha-N-MAX) in Hanks’ Buffer is a commonly used media for Mammalian cell culture.

Advantages

The advantages of MEM Alpha-Modification with Nucleosides MAX (MEM-Alpha-N-MAX) in Hanks’ Buffer over MEM-α-N (HBSS) are:

  • Higher PH Capacity
  • Moreover, it contains 4 mM L-Alanyl-L-Glutamine (0.869 g/L) instead of L-Glutamine and therefore:
  • Possess longer shelf life.
  • More importantly, minimizes toxic ammonia build-up.
  • Significantly improves cell viability and growth.
  • Remains stable across a wide range of temperatures.
  • Consequently, it improves the culture of primary cells.

Mechanism

Although L-glutamine is a vital amino acid, it degrades, resulting in the generation of toxic ammonia and pyrrolidine carboxylic acid. One way to minimize L-glutamine degradation in media is to gradually add these amino acids to your cell culture. However, keeping track of time to keep the amount of L-glutamine at the same level is tedious, if not impossible.

Alternatively, L-alanyl-L-glutamine can be used because it is much more stable in aqueous solutions. More importantly, it does not easily degrade and gradually releases aminopeptidases. As a result, L-glutamine and L-alanine are then used by the cells for protein production in the TCA cycle.

In addition to our Max formulation products, we manufacture PurMaTM GluaSup (Cat# P3S210 559), a combination of L-alanyl-L-glutamine which prevents degradation of L-glutamine.

Contents

MEM Alpha-Modification with Nucleosides MAX (MEM-Alpha-N-MAX) in Hanks’ Buffer contains:

  • 5 mM sodium pyruvate
  • High glucose (4.5g/L)
  • Non-Essential Amino Acids
  • 3.7 g/L Sodium Bicarbonate; PurMa Biologics Manufactures several types of MEM-α-N-MAX (HBSS) based on the amount of Sodium Bicarbonate and HEPES (PH capacity)
  • Phenol Red

PurMa™ MEM Alpha-Modification with Nucleosides MAX (MEM-Alpha-N-MAX) in Hanks’ Buffer is modified from MEM-α-MAX with the following ingredients:

  • Ribonucleosides
  • Deoxyribonucleosides

MEM Alpha-Modification with Nucleosides MAX (MEM-Alpha-N-MAX) in Hanks’ Buffer contains no proteins, lipids, or growth factors. Therefore, it requires supplementation, commonly with 10% Fetal bovine Serum (FBS).

Formulation

The complete formulation can be found here: MEM alpha-MAX with nucleoside in HBSS Buffer Formulation

References

  1. Culture of granulosa cells in collagen gels: the influence of cell shape on steroidogenesis. Carnegie JA, et al. Biol Reprod. 1988 May;38(4):881-90. doi: 10.1095/biolreprod38.4.881. PMID: 3401543
  2. Myelin formation in rat dorsal root ganglion cultured in a serum-free medium. Ninomiya T, Takahashi K. J Anat. 1987 Jun;152:209-13. PMID: 3654371
  3. Long-term organ culture of hamster cheek pouch mucosa. Mock D, Main JH. J Oral Pathol. 1976 Jul;5(4):237-40. doi: 10.1111/j.1600-0714.1976.tb01770.x. PMID: 820846
Parameter Specification
Appearance Red, clear liquid
pH 7.2 ± 0.1
Osmolality 275-360 mOsm/L
Endotoxin NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive Sodium pyruvate
Indicator Phenol red
Mycoplasma Detection Negative
Sterility Tested Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

Additional information

Condition

MEM-α-N-MAX (HBSS) Standard Formulation, MEM-α-N-MAX (HBSS) w/o Phenol Red, MEM-α-N-MAX (HBSS) w/o Sodium Bicarbonate, MEM-α-N-MAX (HBSS) w/o Glucose, MEM-α-N-MAX (HBSS) w/o Lipoic Acid, MEM-α-N-MAX (HBSS) 15 mM HEPES (3.6 g/L), MEM-α-N-MAX (HBSS) 25 mM HEPES (5.9 g/L), MEM-α-N-MAX (HBSS) w/o Sodium Pyruvate, MEM-α-N-MAX (HBSS) High Sodium Bicarbonate (0.85 g/L), MEM-α-N-MAX (HBSS) High Glucose (4.5 g/L), MEM-α-N-MAX (HBSS) 15 mM HEPES (3.6 g/L), High Glucose (4.5 g/L), MEM-α-N-MAX (HBSS) 25 mM HEPES (5.9 g/L), High Glucose (4.5 g/L)
Format

Liquid, Powder
Size

10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L

Documents

Certificate of Analysis (COA): Available upon request for your specific order — email info@purmabiologics.com and we will send the current lot documentation.

Safety Data Sheet (SDS): Available upon request — email info@purmabiologics.com.

What our customers say about PurMa Biologics

Feedback from labs we supply, across our full catalogue.

★★★★★
We buy all of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
Gregory Mullen OU Health Sciences Center
★★★★★
We have been working with PurMa biologics for the past several years, we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum. We appreciate the high quality and reasonable price, and great customer service.
Haus Bio Verified customer
★★★★★
We purchase everything we need related to cell and tissue culture from PurMa, reagents like: DMEM, LL15, EMEM, FBS, trypsin, antibiotics mix100X and... Always great quality, fast processing and shipping. We are totally satisfied.
Verified Customer
★★★★★
Very fast, efficient, and economic service. Great for new PIs like me.
Surendra Shukla OU Health Sciences Center
★★★★★
it is reliable vendor!
Jianhua Song Applied StemCell
★★★★★
We always buy our agarose from PurMa and have never been disappointed.
Zhenghui Liu Applied StemCell

1 review for MEM Alpha-Modification with Nucleosides MAX (MEM-Alpha-N-MAX) in Hanks’ Buffer

  1. Verified Customer, University of Oklahoma –

  2. Verified Customer, University of Oklahoma –

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Frequently asked questions

How should I store MEM Alpha-Modification with Nucleosides MAX (MEM-Alpha-N-MAX) in Hanks’ Buffer?

Store at 2 to 8 degrees C, protected from light, and do not freeze unless the label states otherwise. Freezing can precipitate salts and degrade light sensitive components such as riboflavin and folic acid. Always follow the storage line on the Certificate of Analysis for the specific lot.

What is the shelf life once opened?

Unopened and correctly stored, the typical shelf life is 12 months from manufacture. Once opened, use within about four weeks and always handle in a laminar flow hood, since the practical limit after opening is contamination risk rather than chemical stability.

Is it sterility and endotoxin tested?

Yes. Every lot is sterile filtered and tested for sterility and endotoxin, and a Certificate of Analysis is issued for each lot. The COA is available on request for any catalogue number.

Do I need to add L-glutamine?

It depends on the formulation you order. Standard formulations may contain free L-glutamine, which degrades in solution over time, while MAX formulations use a stable L-alanyl-L-glutamine dipeptide that does not require fresh supplementation. Check the option you have selected before adding more.

Can I order this in bulk or as a custom formulation?

Yes. Bulk volumes and custom formulations are made to order, including adjusted glucose, bicarbonate, buffering and cation content. Use the request bulk pricing button on this page and include the specification you need.

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